PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

Total Entries - 11
Entry No. - 1 [TOP]

PolysacDB ID	1471
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Helicobacter pylori (NCBI Taxonomy) (Drugpedia)
Basic Structure	The structure of the O-polysaccharide part of the LPS is the same as the Lewis X (Lex) and Lewis Y (Ley) determinants of the human cell surface glycoconjugates
BCSDB Structure	6366
Proposed functions	The O-polysaccharide regions of these LPS were found to be a major antigenic determinant
Antigenic Nature used to produce antibodies	Naturally infected patiens
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Type A Polysera
Antibody type and class	N/A
Assay System	Immunoblotting and ELISA
Cross-reactivity	This antisera reacted with the polysaccharides of GU2, DU1 and CA1 LPS only
Proposed epitopes	N/A
IEDB Epitope	76711
Proposed Utility	This antisera may yield useful information concerning the real surface of the bacterium
Curator ID	AA + AS
Date of Curation	27-07-2010
References	10603381

Entry No. - 2 [TOP]

PolysacDB ID	1472
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Helicobacter pylori (NCBI Taxonomy) (Drugpedia)
Basic Structure	The structure of the O-polysaccharide part of the LPS is the same as the Lewis X (Lex) and Lewis Y (Ley) determinants of the human cell surface glycoconjugates
BCSDB Structure	6366
Proposed functions	The O-polysaccharide regions of these LPS were found to be a major antigenic determinant
Antigenic Nature used to produce antibodies	N/A
Carrier Name	N/A
Conjugation Method	N/A
Antibodies	Mab 73-30
Antibody type and class	IgM
Assay System	Immunoblotting and ELISA
Cross-reactivity	This anti-Lex Mab reacted with strains DU1, DU2, GU2 and CA6, GU9 and CA4 and did not react with strains 43504-r and CG7
Proposed epitopes	N/A
IEDB Epitope	76711
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	28-07-2010
References	PMC170591

Entry No. - 3 [TOP]

PolysacDB ID	1473
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Helicobacter pylori (NCBI Taxonomy) (Drugpedia)
Basic Structure	The structure of the O-polysaccharide part of the LPS is the same as the Lewis X (Lex) and Lewis Y (Ley) determinants of the human cell surface glycoconjugates
BCSDB Structure	6366
Proposed functions	The O-polysaccharide regions of these LPS were found to be a major antigenic determinant
Antigenic Nature used to produce antibodies	N/A
Carrier Name	N/A
Conjugation Method	N/A
Antibodies	Mab BG-7 (P12)
Antibody type and class	IgM
Assay System	Immunoblotting and ELISA
Cross-reactivity	This anti-Lex Mab reacted with strains DU1, DU2, GU2 and CA6, GU9 and CA4 and did not react with strains 43504-r and CG7
Proposed epitopes	N/A
IEDB Epitope	76711
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	28-07-2010
References	PMC170591

Entry No. - 4 [TOP]

PolysacDB ID	1474
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Helicobacter pylori (NCBI Taxonomy) (Drugpedia)
Basic Structure	The structure of the O-polysaccharide part of the LPS is the same as the Lewis X (Lex) and Lewis Y (Ley) determinants of the human cell surface glycoconjugates
BCSDB Structure	6366
Proposed functions	The O-polysaccharide regions of these LPS were found to be a major antigenic determinant
Antigenic Nature used to produce antibodies	N/A
Carrier Name	N/A
Conjugation Method	N/A
Antibodies	Mab H18A
Antibody type and class	IgG3
Assay System	Immunoblotting and ELISA
Cross-reactivity	This anti-Lex Mab reacted with strains CG4 and CA2, CG9 and GU10 and did not react with strains 43504-r and CG7
Proposed epitopes	N/A
IEDB Epitope	76711
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	28-07-2010
References	PMC170591

Entry No. - 5 [TOP]

PolysacDB ID	1475
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Helicobacter pylori (NCBI Taxonomy) (Drugpedia)
Basic Structure	The structure of the O-polysaccharide part of the LPS is the same as the Lewis X (Lex) and Lewis Y (Ley) determinants of the human cell surface glycoconjugates
BCSDB Structure	6366
Proposed functions	The O-polysaccharide regions of these LPS were found to be a major antigenic determinant
Antigenic Nature used to produce antibodies	N/A
Carrier Name	N/A
Conjugation Method	N/A
Antibodies	Mab BG-8 (F3)
Antibody type and class	IgM
Assay System	Immunoblotting and ELISA
Cross-reactivity	This anti-Lex Mab reacted with strains CG4 and CA2, CG9 and GU10 and did not react with strains 43504-r and CG7
Proposed epitopes	N/A
IEDB Epitope	76711
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	29-07-2010
References	PMC170591

Entry No. - 6 [TOP]

PolysacDB ID	1476
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Helicobacter pylori (NCBI Taxonomy) (Drugpedia)
Basic Structure	The structure of the O-polysaccharide part of the LPS is the same as the Lewis X (Lex) and Lewis Y (Ley) determinants of the human cell surface glycoconjugates
BCSDB Structure	6366
Proposed functions	The O-polysaccharide regions of these LPS were found to be a major antigenic determinant
Antigenic Nature used to produce antibodies	N/A
Carrier Name	N/A
Conjugation Method	N/A
Antibodies	MAB 2108 (7LE)
Antibody type and class	IgG1
Assay System	Immunoblotting and ELISA
Cross-reactivity	This anti-Lex Mab reacted with strains CG9 and GU10, GU2 and CA6 and did not react with strains 43504-r and CG7
Proposed epitopes	N/A
IEDB Epitope	76711
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	29-07-2010
References	PMC170591

Entry No. - 7 [TOP]

PolysacDB ID	1477
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Helicobacter pylori (NCBI Taxonomy) (Drugpedia)
Basic Structure	The structure of the O-polysaccharide part of the LPS is the same as the Lewis X (Lex) and Lewis Y (Ley) determinants of the human cell surface glycoconjugates
BCSDB Structure	6366
Proposed functions	The O-polysaccharide regions of these LPS were found to be a major antigenic determinant
Antigenic Nature used to produce antibodies	N/A
Carrier Name	N/A
Conjugation Method	N/A
Antibodies	Mab BG-5 (T174)
Antibody type and class	IgG1
Assay System	Immunoblotting and ELISA
Cross-reactivity	This anti-Lex Mab reacted with strains CG9 and GU10, GU2 and CA6 and did not react with strains 43504-r and CG7
Proposed epitopes	N/A
IEDB Epitope	76711
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	29-07-2010
References	PMC170591

Entry No. - 8 [TOP]

PolysacDB ID	1478
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Helicobacter pylori (NCBI Taxonomy) (Drugpedia)
Basic Structure	The structure of the O-polysaccharide part of the LPS is the same as the Lewis X (Lex) and Lewis Y (Ley) determinants of the human cell surface glycoconjugates
BCSDB Structure	6366
Proposed functions	The O-polysaccharide regions of these LPS were found to be a major antigenic determinant
Antigenic Nature used to produce antibodies	N/A
Carrier Name	N/A
Conjugation Method	N/A
Antibodies	Mab BG-6 (T218)
Antibody type and class	IgM
Assay System	Immunoblotting and ELISA
Cross-reactivity	This anti-Lex Mab reacted with strains CG4 and CA2, CG9 and GU10 and did not react with strains 43504-r and CG7
Proposed epitopes	N/A
IEDB Epitope	76711
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	29-07-2010
References	PMC170591

Entry No. - 9 [TOP]

PolysacDB ID	1479
Carbohydrate Name	Le^x carbohydrate (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Helicobacter pylori (NCBI Taxonomy) (Drugpedia)
Basic Structure	The structure consists of the following residues : (Galβ1-->4[Fucα1-->3]GlcNAc
BCSDB Structure	10702
Proposed functions	N/A
Antigenic Nature used to produce antibodies	N/A
Carrier Name	N/A
Conjugation Method	N/A
Antibodies	Mab 85-5000
Antibody type and class	IgM
Assay System	Immunoblotting, immunoelectron microscopy and ELISA
Cross-reactivity	This Lex specific Mab cross-reacted with H. pylori strains NCTC11637, UA799, UA802, UA825, UA861, UA1182, and UA1206
Proposed epitopes	Galβ1-->4[Fucα1-->3]GlcNAc
IEDB Epitope	76711
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	30-07-2010
References	PMC173655

Entry No. - 10 [TOP]

PolysacDB ID	1480
Carbohydrate Name	Le^x carbohydrate (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Helicobacter pylori (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	N/A
Carrier Name	N/A
Conjugation Method	N/A
Antibodies	Mab CNM 1336
Antibody type and class	IgM
Assay System	Immunoblotting, immunoelectron microscopy and ELISA
Cross-reactivity	This Lex specific Mab cross-reacted with H. pylori strains NCTC11637, UA799, UA802, UA825, UA861, UA1182, and UA1206
Proposed epitopes	Galβ1-->4[Fucα1-->3]GlcNAcβ1-->3Galβ1-->4Glc
IEDB Epitope	76711
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	30-07-2010
References	PMC173655

Entry No. - 11 [TOP]

PolysacDB ID	1481
Carbohydrate Name	Galβ1-->4[Fucα1-->3]GlcNAcβ1--> 3Galβ1-->4[Fucα1-->3]GlcNAc (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Helicobacter pylori (NCBI Taxonomy) (Drugpedia)
Basic Structure	The structure consists of the following residues : Galβ1-->4[Fucα1-->3]GlcNAcβ1--> 3Galβ1-->4[Fucα1-->3]GlcNAc
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	N/A
Carrier Name	N/A
Conjugation Method	N/A
Antibodies	Mab BCR 90/45
Antibody type and class	IgM
Assay System	Immunoblotting, immunoelectron microscopy and ELISA
Cross-reactivity	This Lex specific Mab cross-reacted with H. pylori strains NCTC11637, UA799, UA802, UA825, UA861, UA1182, and UA1206
Proposed epitopes	N/A
IEDB Epitope	76711
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	30-07-2010
References	PMC173655

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