PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 2

Entry No. - 1   [TOP]
PolysacDB ID1004
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeHaemophilus influenzae type B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureA relatively simple antigen consisting of repeating units of 3-β-D ribose-(1-->1)-D-ribitol-5-phosphate
BCSDB Structure23423
Proposed functionsAn important virulence determinant
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameNeisseria meningitidis group B outer membrane protein complex (Hib PS-OMP)
Conjugation MethodThe strategy uses a oligopeptide spacer molecule whose parts are derived from both modified Haemophilus influenzae capsular polysaccharide and Neisseria meningitides group B outer membrane complex
AntibodiesPolysera
Antibody type and classIgG1 and IgG2
Assay SystemN\A
Cross-reactivityN/A
Proposed epitopesN\A
IEDB EpitopeN/A
Proposed UtilityThese antibodies exert their protective effect by initiating complement-mediated activities including opsonization and bacterial lysis
Curator IDAA + AS
Date of Curation01-01-2010
ReferencesPMC296547


Entry No. - 2   [TOP]
PolysacDB ID1005
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeHaemophilus influenzae type B   (NCBI Taxonomy)   (Drugpedia)
Basic StructureA relatively simple antigen consisting of repeating units of 3-β-D ribose-(1-->1)-D-ribitol-5-phosphate
BCSDB Structure23423
Proposed functionsAn important virulence determinant
Antigenic Nature used to produce antibodiesGlycoconjugates
Carrier NameTetanus toxoid
Conjugation MethodProtein solutions (25 mg/ml) and Adipic acid dihydrazide [ADH] (3.45 rag/rag protein) were reacted with three different concentrations of EDAC (0 1, 0.3, and 0.6 rag/rag protein) The pH of the reaction mixture was maintained at 4.7 ± 0.2 with 0.1 N HC! The reaction proceeded at room temperature for 3 h and the reaction mixtures were dialyzed at 3-8°C with two changes/d against 6 liter of 0 2 M NaCI. The albumin and polysaccharide derivatives were then dialyzed against two 6-liter changes of deionized water and freeze-dried. The polysaccharide was activated with CNBr. Briefly, a solution of polysaccharide (5.0 mg/ml), equilibrated at 4°C, was rapidly brought to pH 10.5 with 0.1 N NaOH. 100 mg/ml CNBr was added to a final concentration of 0.4 mg/mg polysaccharide, and the pH maintained at 10.5 for 6 rain. Then the reaction mixture was brought to pH 8 5 with 0.5 M NaHCO3, and the CNBr-actlvated polysaccharide added to an equal weight of ADH-protein. The reaction mixture was tumbled gently overnight at 3-8°C and then centrifuged at 16,000 g, 4°C for 20 ram. The supernatant was passed through a CL-4B Sepharose column, 1 5 × 90 cm, that was equihbrated with 0 2 M ammonium acetate. The void-volume fractions were pooled, dialyzed against 0.01 M phosphate-buffered 0.145 M NaCI, pH 7.0, at 3-8°C, and passed through a 045-nm membrane and stored at 3-8°C
AntibodiesPolysera
Antibody type and classIgG and IgA
Assay SystemRadioimmunoassay
Cross-reactivityN/A
Proposed epitopesN\A
IEDB EpitopeN/A
Proposed UtilityThese antibodies exert their protective effect by initiating complement-mediated activities including opsonization and bacterial lysis
Curator IDAA + AS
Date of Curation02-01-2010
ReferencesPMC2185954


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