PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 3

Entry No. - 1   [TOP]
PolysacDB ID1263
Carbohydrate NameGlucomannoprotein   (Drugpedia)
Carbohydrate ClassGlycoprotein
MicrobeCandida albicans serotype A   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesGlucomannoprotein-rich antigenic extract (GMP)
Carrier NameNil
Conjugation MethodNil
AntibodiesMab AF-1
Antibody type and classIgM
Assay SystemELISA, immunofluorescence and slide agglutination tests
Cross-reactivityThis Mab cross-reacted with C. albicans serotypes A and B, C. tropicalis, C. guilliermondii and C. viswanathii, but did not cross-react with C. krusei, C. parapsilosis, C. kefyr (pseudotropicalis) and T. Glabrata
Proposed epitopesThe epitope was proposed to consist of mannan residues
IEDB EpitopeN/A
Proposed UtilityThis Mab had strong agglutination properties for Candida species. This Mab helped in the identification of new polysaccharide epitopes on the cell wall of pathogenic Candida species.
Curator IDAA + AS
Date of Curation02-05-2010
References2462051


Entry No. - 2   [TOP]
PolysacDB ID1351
Carbohydrate NameMannan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeCandida albicans serotype A   (NCBI Taxonomy)   (Drugpedia)
Basic StructureAn α-linked branched homopolymer of mannose linked to protein
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat-killed cell suspension of C. albicans M1012(serotype A)
Carrier NameNil
Conjugation MethodNil
AntibodiesMab CA4-2
Antibody type and classIgM
Assay SystemSlide agglutination tests, immunofluorescence
Cross-reactivityThis Mab cross-reacted with C. tropicalis, and T. glabrata strains
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab had agglutinating properties and may be useful also as reagents for revealing the immunochemical structures of specific determinants of C. albiccanis cell wall mannans
Curator IDAA + AS
Date of Curation26-06-2010
ReferencesPMC268742


Entry No. - 3   [TOP]
PolysacDB ID1352
Carbohydrate NameMannan   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeCandida albicans serotype A   (NCBI Taxonomy)   (Drugpedia)
Basic StructureAn α-linked branched homopolymer of mannose linked to protein
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesHeat-killed cell suspension of C. albicans M1012(serotype A)
Carrier NameNil
Conjugation MethodNil
AntibodiesMab CA5-4
Antibody type and classIgM
Assay SystemSlide agglutination tests, immunofluorescence
Cross-reactivityThis Mab cross-reacted with C. albicans serotype B, and C. tropicalis strains
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab had agglutinating properties and may be useful also as reagents for revealing the immunochemical structures of specific determinants of C. albicans cell wall mannans
Curator IDAA + AS
Date of Curation26-06-2010
ReferencesPMC268742


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