PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

Total Entries - 15
Entry No. - 1 [TOP]

PolysacDB ID	1014
Carbohydrate Name	Glucan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Candida albicans (NCBI Taxonomy) (Drugpedia)
Basic Structure	Glucans are a heterogeneous group of glucose polymers, consisting of a backbone of β(1-->3)-linked β-D-glucopyranosyl units with β(1-->6)-linked side chains of varying distribution and length
BCSDB Structure	125281
Proposed functions	Glucan is an essential cell wall component in pathogenic fungi and plays a critical role in cell viability
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Diphtheria toxoid CRM 197
Conjugation Method	A 20-fold molar excess of activated polysaccharide was reacted overnight with the protein, at room temperature, in 10 mM phosphate buffer, pH 7.2. The unbound polysaccharide was separated from the glyco-conjugate by ultrafiltration, using Amicon 10-kD filter devices
Antibodies	Mab 2G8
Antibody type and class	IgG2
Assay System	Flow Cytometry, confocal microscopy, phospholipase assay, yeast growth inhibition assay
Cross-reactivity	This antibody binds both C. neoformans and Candida albicans
Proposed epitopes	N\A
IEDB Epitope	76671
Proposed Utility	MAb 2G8 inhibits the in vitro growth of C. neoformans and also exerts anti-C. neoformans protective effects in vivo.
Curator ID	AA + AS
Date of Curation	04-01-2010
References	PMC2168274

Entry No. - 2 [TOP]

PolysacDB ID	1015
Carbohydrate Name	Beta-glucan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Candida albicans (NCBI Taxonomy) (Drugpedia)
Basic Structure	Glucans are a heterogeneous group of glucose polymers, consisting of a backbone of β(1-->3)-linked β-D-glucopyranosyl units with β(1-->6)-linked side chains of varying distribution and length
BCSDB Structure	125281
Proposed functions	Glucan is an essential cell wall component in pathogenic fungi and plays a critical role in cell viability
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Diphtheria toxoid CRM 197
Conjugation Method	A 20-fold molar excess of activated polysaccharide was reacted overnight with the protein, at room temperature, in 10 mM phosphate buffer, pH 7.2. The unbound polysaccharide was separated from the glyco-conjugate by ultrafiltration, using Amicon 10-kD filter devices
Antibodies	Polysera
Antibody type and class	IgG
Assay System	Invivo protection assays, growth inhibition assays, indirect ELISA
Cross-reactivity	N/A
Proposed epitopes	N\A
IEDB Epitope	76671
Proposed Utility	The Lam-CRM conjugate is immunogenic and protective against systemic candidiasis in mice. The antibodies induce passive protection in mice against systemic and mucosal candidiasis
Curator ID	AA + AS
Date of Curation	04-01-2010
References	PMC2212864

Entry No. - 3 [TOP]

PolysacDB ID	1265
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Candida albicans (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab C6
Antibody type and class	IgM
Assay System	ELISA, immunoelecton microscopy and agglutination tests
Cross-reactivity	This antibody cross-reacted with 30 strains of Candida albicans strains, 2 strains of Candida tropicalis but did not cross-react with Candida paratropicalis, Candida parapsilosis, T. glabrata and S. cerevisae.
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab had a agglutinating activity against Candida albicans
Curator ID	AA + AS
Date of Curation	02-05-2010
References	3280163

Entry No. - 4 [TOP]

PolysacDB ID	1266
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Candida albicans (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Fractions enriched for mitochondria and other membranous vesicles [membranous fraction] of Candida albicans
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 10G
Antibody type and class	IgM
Assay System	ELISA, immunocollidal gold electron microscopy, indirect immunofuorescence assay, immunoelectrophoresis
Cross-reactivity	Yeast cells of 15 additional C. albicans strains and isolates of C. stellatoidka, C. tropicalis, C. intermedia and C. lusitaniae were also agglutinated by mAb 1OG. The antigen was not detected on other fungi, including Can&& krusei, C.utilis, Cryptococcus neoformans, Cr. albidus, Torulopsis glabrata, Rhodotorula spp. and Saccharomyces cerevisiae
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab had a agglutinating activity against Candida albicans
Curator ID	AA + AS
Date of Curation	03-05-2010
References	3280163

Entry No. - 5 [TOP]

PolysacDB ID	1330
Carbohydrate Name	Phosphomannan Complex (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Candida albicans (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab B6.1
Antibody type and class	IgM
Assay System	ELISA, Immunofluorescence and immunoblotting
Cross-reactivity	N/A
Proposed epitopes	β-1,2-mannotriose residues of the L-mannan constitute an important part of the epitope
IEDB Epitope	76920
Proposed Utility	MAb C3.1 transfers protection against disseminated candidiasis
Curator ID	AA + AS
Date of Curation	14-06-2010
References	PMC97325

Entry No. - 6 [TOP]

PolysacDB ID	1331
Carbohydrate Name	Phosphomannan Complex (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Candida albicans (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab B6
Antibody type and class	IgM
Assay System	ELISA, Immunofluorescence and immunoblotting
Cross-reactivity	N/A
Proposed epitopes	N/A
IEDB Epitope	76920
Proposed Utility	This Mab can be used for epitope mapping of non-immunodominant residues of L-mannan
Curator ID	AA + AS
Date of Curation	16-06-2010
References	PMC97325

Entry No. - 7 [TOP]

PolysacDB ID	1332
Carbohydrate Name	Phosphomannan Complex (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Candida albicans (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab C3.1
Antibody type and class	IgG3
Assay System	ELISA, Immunofluorescence and immunoblotting
Cross-reactivity	N/A
Proposed epitopes	β-1,2-mannotriose residues of the L-mannan constitute an important part of the epitope
IEDB Epitope	76920
Proposed Utility	MAb C3.1 transfers protection against disseminated candidiasis
Curator ID	AA + AS
Date of Curation	16-06-2010
References	PMC97325

Entry No. - 8 [TOP]

PolysacDB ID	1336
Carbohydrate Name	Human Cd15 (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Candida albicans (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 7C3
Antibody type and class	N/A
Assay System	ELISA, Immunofluorescence
Cross-reactivity	This Mab against a human antigen cross-reacted with Candida albicans species
Proposed epitopes	The epitope is supposed to contain lacto-N-fucopentaose III
IEDB Epitope	N/A
Proposed Utility	This Mab helped in epitope elucidation of the mannans present on Candidial surfaces
Curator ID	AA + AS
Date of Curation	18-06-2010
References	PMC313726

Entry No. - 9 [TOP]

PolysacDB ID	1337
Carbohydrate Name	Phosphopeptidomannan (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Candida albicans (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab EB-CA1
Antibody type and class	N/A
Assay System	ELISA, immunoblotting
Cross-reactivity	N/A
Proposed epitopes	The probable epitope consists of 1 to more than 11 mannosyl residues and linked mainly through α(1-->2) and α(1-->3) bonds
IEDB Epitope	N/A
Proposed Utility	This Mab helped in epitope elucidation of the mannans present on Candidial surfaces
Curator ID	AA + AS
Date of Curation	19-06-2010
References	PMC257398

Entry No. - 10 [TOP]

PolysacDB ID	1338
Carbohydrate Name	Phosphopeptidomannan (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Candida albicans (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab EB-CA2
Antibody type and class	N/A
Assay System	ELISA, immunoblotting
Cross-reactivity	N/A
Proposed epitopes	The probable epitope consists of 1 to more than 11 mannosyl residues and linked mainly through α(1-->2) and α(1-->3) bonds
IEDB Epitope	N/A
Proposed Utility	This Mab helped in epitope elucidation of the mannans present on Candidial surfaces
Curator ID	AA + AS
Date of Curation	19-06-2010
References	PMC257398

Entry No. - 11 [TOP]

PolysacDB ID	1339
Carbohydrate Name	Phosphopeptidomannan (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Candida albicans (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab H5
Antibody type and class	N/A
Assay System	ELISA, immunoblotting
Cross-reactivity	N/A
Proposed epitopes	The probable epitope consists of 1 to more than 11 mannosyl residues and linked mainly through α(1-->2) and α(1-->3) bonds
IEDB Epitope	N/A
Proposed Utility	This Mab helped in epitope elucidation of the mannans present on Candidial surfaces
Curator ID	AA + AS
Date of Curation	19-06-2010
References	PMC257398

Entry No. - 12 [TOP]

PolysacDB ID	1340
Carbohydrate Name	Phosphopeptidomannan (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Candida albicans (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab AF1
Antibody type and class	N/A
Assay System	ELISA, immunoblotting
Cross-reactivity	N/A
Proposed epitopes	The epitope consiosts of mannosyl residues from mannobiose to mannoheptaose are bound through β(1-->2) linkage
IEDB Epitope	N/A
Proposed Utility	This Mab helped in epitope elucidation of the mannans present on Candidial surfaces
Curator ID	AA + AS
Date of Curation	22-06-2010
References	PMC257398

Entry No. - 13 [TOP]

PolysacDB ID	1341
Carbohydrate Name	Phosphopeptidomannan (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Candida albicans (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab C6
Antibody type and class	N/A
Assay System	ELISA, immunoblotting
Cross-reactivity	N/A
Proposed epitopes	The epitope consiosts of mannosyl residues from mannobiose to mannoheptaose are bound through β(1-->2) linkage
IEDB Epitope	N/A
Proposed Utility	This Mab helped in epitope elucidation of the mannans present on Candidial surfaces
Curator ID	AA + AS
Date of Curation	22-06-2010
References	PMC257398

Entry No. - 14 [TOP]

PolysacDB ID	1342
Carbohydrate Name	Phosphopeptidomannan (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Candida albicans (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 5B2
Antibody type and class	N/A
Assay System	ELISA, immunoblotting
Cross-reactivity	N/A
Proposed epitopes	The epitope consists of mannopyranosyl units bound through β(1-->2) linkages present both within the oligomannoside H pool and at the nonreducing end of some oligomannosides A
IEDB Epitope	N/A
Proposed Utility	This Mab helped in epitope elucidation of the mannans present on Candidial surfaces
Curator ID	AA + AS
Date of Curation	23-06-2010
References	PMC257398

Entry No. - 15 [TOP]

PolysacDB ID	1344
Carbohydrate Name	Unknown carbohydrate (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Candida albicans (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Live C. albicans blastoconidia
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 5B2
Antibody type and class	IgM
Assay System	Immunofluorescence and precipitin reactions
Cross-reactivity	This Mab reacted with C. albicans, C. tropic alis, C. glabr-ata, and C. Giiillierrnondii
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab had the ability to agglutinate Candida species
Curator ID	AA + AS
Date of Curation	24-06-2010
References	PMC260140

Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020