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PolysacDB ID | 1202 |
Carbohydrate Name | Smooth Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab12G12 |
Antibody type and class | N/A |
Assay System | ELISA, immunogold stain labelling and immunoblotting |
Cross-reactivity | This Mab reacted with smooth Brucellu abortus strain 99 and the smooth B. melitensis strain 16M |
Proposed epitopes | This Mab was proposed to be directed to antigenic determinants present along the length of the polymeric O-chain |
IEDB Epitope | N/A |
Proposed Utility | N/A |
Curator ID | AA + AS |
Date of Curation | 06-03-2010 |
References | 1382111 |
PolysacDB ID | 1203 |
Carbohydrate Name | Rough Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | N/A |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab A68 |
Antibody type and class | N/A |
Assay System | ELISA, immunogold stain labelling and immunoblotting |
Cross-reactivity | This antibody was specific to R-LPS of B. melitensis |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | N/A |
Curator ID | AA + AS |
Date of Curation | 06-03-2010 |
References | 1382111 |
PolysacDB ID | 1204 |
Carbohydrate Name | Smooth Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M |
BCSDB Structure | N/A |
Proposed functions | LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 05D01 |
Antibody type and class | IgG1 |
Assay System | ELISA and immunoblotting |
Cross-reactivity | This antibody was specific to a common epitope on A and M polysaccharides. This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS |
Proposed epitopes | This Mab was proposed to be directed to antigenic determinants present along the length of the polymeric O-chain |
IEDB Epitope | N/A |
Proposed Utility | N/A |
Curator ID | AA + AS |
Date of Curation | 07-03-2010 |
References | 1382111 |
PolysacDB ID | 1205 |
Carbohydrate Name | Smooth Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M |
BCSDB Structure | N/A |
Proposed functions | LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 07F09 |
Antibody type and class | IgG1 |
Assay System | ELISA and immunoblotting |
Cross-reactivity | This antibody was specific to a common epitope on A and M polysaccharides. This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS |
Proposed epitopes | Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope |
IEDB Epitope | N/A |
Proposed Utility | This mAb may be particularly useful for typingsmooth Brucella strains |
Curator ID | AA + AS |
Date of Curation | 07-03-2010 |
References | 1382111 |
PolysacDB ID | 1206 |
Carbohydrate Name | Smooth Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M |
BCSDB Structure | N/A |
Proposed functions | LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 13A09 |
Antibody type and class | IgG1 |
Assay System | ELISA and immunoblotting |
Cross-reactivity | This antibody was specific to a common epitope on A and M polysaccharides. This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS |
Proposed epitopes | Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope |
IEDB Epitope | N/A |
Proposed Utility | This mAb may be particularly useful for typingsmooth Brucella strains |
Curator ID | AA + AS |
Date of Curation | 07-03-2010 |
References | 1382111 |
PolysacDB ID | 1207 |
Carbohydrate Name | Smooth Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M |
BCSDB Structure | N/A |
Proposed functions | LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 14f12 |
Antibody type and class | IgG1 |
Assay System | ELISA and immunoblotting |
Cross-reactivity | This antibody was specific to a common epitope on A and M polysaccharides. This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS |
Proposed epitopes | Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope |
IEDB Epitope | N/A |
Proposed Utility | This mAb may be particularly useful for typingsmooth Brucella strains |
Curator ID | AA + AS |
Date of Curation | 08-03-2010 |
References | 1382111 |
PolysacDB ID | 1208 |
Carbohydrate Name | Smooth Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M |
BCSDB Structure | N/A |
Proposed functions | LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 16F01 |
Antibody type and class | IgG1 |
Assay System | ELISA and immunoblotting |
Cross-reactivity | This antibody was specific to a common epitope on A and M polysaccharides. This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS |
Proposed epitopes | Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope |
IEDB Epitope | N/A |
Proposed Utility | This mAb may be particularly useful for typingsmooth Brucella strains |
Curator ID | AA + AS |
Date of Curation | 09-03-2010 |
References | 1382111 |
PolysacDB ID | 1209 |
Carbohydrate Name | Smooth Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M |
BCSDB Structure | N/A |
Proposed functions | LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 13G11 |
Antibody type and class | IgM |
Assay System | ELISA and immunoblotting |
Cross-reactivity | This antibody was specific to a common epitope on A and M polysaccharides. This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS |
Proposed epitopes | Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope |
IEDB Epitope | N/A |
Proposed Utility | This mAb may be particularly useful for typingsmooth Brucella strains |
Curator ID | AA + AS |
Date of Curation | 09-03-2010 |
References | 1382111 |
PolysacDB ID | 1210 |
Carbohydrate Name | Smooth Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M |
BCSDB Structure | N/A |
Proposed functions | LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 05E11 |
Antibody type and class | IgM |
Assay System | ELISA and immunoblotting |
Cross-reactivity | This antibody was specific to a common epitope on A and M polysaccharides. This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS |
Proposed epitopes | Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope |
IEDB Epitope | N/A |
Proposed Utility | This mAb may be particularly useful for typingsmooth Brucella strains |
Curator ID | AA + AS |
Date of Curation | 10-03-2010 |
References | 1382111 |
PolysacDB ID | 1211 |
Carbohydrate Name | Smooth Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M |
BCSDB Structure | N/A |
Proposed functions | LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 14H09 |
Antibody type and class | IgM |
Assay System | ELISA and immunoblotting |
Cross-reactivity | This antibody was specific to a common epitope on A and M polysaccharides. This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS |
Proposed epitopes | Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope |
IEDB Epitope | N/A |
Proposed Utility | This mAb may be particularly useful for typingsmooth Brucella strains |
Curator ID | AA + AS |
Date of Curation | 10-03-2010 |
References | 1382111 |
PolysacDB ID | 1212 |
Carbohydrate Name | Smooth Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M |
BCSDB Structure | N/A |
Proposed functions | LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 15B10 |
Antibody type and class | IgG2a |
Assay System | ELISA and immunoblotting |
Cross-reactivity | This antibody was specific to a common epitope on A and M polysaccharides. But was more reactive towards M dominant strain B. melitensis 16M. This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS |
Proposed epitopes | Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope |
IEDB Epitope | N/A |
Proposed Utility | This mAb may be particularly useful for typingsmooth Brucella strains |
Curator ID | AA + AS |
Date of Curation | 11-03-2010 |
References | 1382111 |
PolysacDB ID | 1213 |
Carbohydrate Name | Smooth Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M |
BCSDB Structure | N/A |
Proposed functions | LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 02B05 |
Antibody type and class | IgG3 |
Assay System | ELISA and immunoblotting |
Cross-reactivity | This antibody was specific to a common epitope on A and M polysaccharides. But was more reactive towards M dominant strain B. melitensis 16M. This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS |
Proposed epitopes | Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope |
IEDB Epitope | N/A |
Proposed Utility | This mAb may be particularly useful for typingsmooth Brucella strains |
Curator ID | AA + AS |
Date of Curation | 11-03-2010 |
References | 1382111 |
PolysacDB ID | 1214 |
Carbohydrate Name | Smooth Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M |
BCSDB Structure | N/A |
Proposed functions | LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 03D03 |
Antibody type and class | IgG3 |
Assay System | ELISA and immunoblotting |
Cross-reactivity | This antibody was specific to a common epitope on A and M polysaccharides. But was more reactive towards M dominant strain B. melitensis 16M.This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS |
Proposed epitopes | Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope |
IEDB Epitope | N/A |
Proposed Utility | This mAb may be particularly useful for typingsmooth Brucella strains |
Curator ID | AA + AS |
Date of Curation | 12-03-2010 |
References | 1382111 |
PolysacDB ID | 1215 |
Carbohydrate Name | Smooth Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M |
BCSDB Structure | N/A |
Proposed functions | LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 04A05 |
Antibody type and class | IgG3 |
Assay System | ELISA and immunoblotting |
Cross-reactivity | This antibody was specific to a common epitope on A and M polysaccharides. But was more reactive towards M dominant strain B. melitensis 16M.This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS |
Proposed epitopes | Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope |
IEDB Epitope | N/A |
Proposed Utility | This mAb may be particularly useful for typingsmooth Brucella strains |
Curator ID | AA + AS |
Date of Curation | 12-03-2010 |
References | 1382111 |
PolysacDB ID | 1216 |
Carbohydrate Name | Smooth Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M |
BCSDB Structure | N/A |
Proposed functions | LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 11H03 |
Antibody type and class | IgG3 |
Assay System | ELISA and immunoblotting |
Cross-reactivity | This antibody was specific to a common epitope on A and M polysaccharides. But was more reactive towards M dominant strain B. melitensis 16M.This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS |
Proposed epitopes | Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope |
IEDB Epitope | N/A |
Proposed Utility | This mAb may be particularly useful for typingsmooth Brucella strains |
Curator ID | AA + AS |
Date of Curation | 12-03-2010 |
References | 1382111 |
PolysacDB ID | 1217 |
Carbohydrate Name | Smooth Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M |
BCSDB Structure | N/A |
Proposed functions | LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 12B12 |
Antibody type and class | IgG3 |
Assay System | ELISA and immunoblotting |
Cross-reactivity | This antibody was specific to a common epitope on A and M polysaccharides. But was more reactive towards M dominant strain B. melitensis 16M.This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS |
Proposed epitopes | Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope |
IEDB Epitope | N/A |
Proposed Utility | This mAb may be particularly useful for typingsmooth Brucella strains |
Curator ID | AA + AS |
Date of Curation | 19-03-2010 |
References | 1382111 |
PolysacDB ID | 1218 |
Carbohydrate Name | Smooth Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M |
BCSDB Structure | N/A |
Proposed functions | LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 17H02 |
Antibody type and class | IgG3 |
Assay System | ELISA and immunoblotting |
Cross-reactivity | This antibody was specific to a common epitope on A and M polysaccharides. But was more reactive towards M dominant strain B. melitensis 16M.This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS |
Proposed epitopes | Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope |
IEDB Epitope | N/A |
Proposed Utility | This mAb may be particularly useful for typingsmooth Brucella strains |
Curator ID | AA + AS |
Date of Curation | 19-03-2010 |
References | 1382111 |
PolysacDB ID | 1219 |
Carbohydrate Name | Smooth Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M |
BCSDB Structure | N/A |
Proposed functions | LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 01F08 |
Antibody type and class | IgG3 |
Assay System | ELISA and immunoblotting |
Cross-reactivity | This antibody was specific to a common epitope on A and M polysaccharides. But was more reactive towards M dominant strain B. melitensis 16M.This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS |
Proposed epitopes | Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope |
IEDB Epitope | N/A |
Proposed Utility | This mAb may be particularly useful for typingsmooth Brucella strains |
Curator ID | AA + AS |
Date of Curation | 19-03-2010 |
References | 1382111 |
PolysacDB ID | 1220 |
Carbohydrate Name | Smooth Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M |
BCSDB Structure | N/A |
Proposed functions | LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 08C06 |
Antibody type and class | IgG3 |
Assay System | ELISA and immunoblotting |
Cross-reactivity | This antibody was specific to a common epitope on A and M polysaccharides. But was more reactive towards M dominant strain B. melitensis 16M.This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS |
Proposed epitopes | Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope |
IEDB Epitope | N/A |
Proposed Utility | This mAb may be particularly useful for typingsmooth Brucella strains |
Curator ID | AA + AS |
Date of Curation | 19-03-2010 |
References | 1382111 |
PolysacDB ID | 1221 |
Carbohydrate Name | Smooth Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M |
BCSDB Structure | N/A |
Proposed functions | LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 08B07 |
Antibody type and class | IgG3 |
Assay System | ELISA and immunoblotting |
Cross-reactivity | This antibody was specific to a common epitope on A and M polysaccharides. But was more reactive towards M dominant strain B. melitensis 16M.This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS |
Proposed epitopes | Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope |
IEDB Epitope | N/A |
Proposed Utility | This mAb may be particularly useful for typingsmooth Brucella strains |
Curator ID | AA + AS |
Date of Curation | 19-03-2010 |
References | 1382111 |
PolysacDB ID | 1222 |
Carbohydrate Name | Smooth Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M |
BCSDB Structure | N/A |
Proposed functions | LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 03E05 |
Antibody type and class | IgG3 |
Assay System | ELISA and immunoblotting |
Cross-reactivity | This antibody was highly specific for S-LPS of the M dominant strain (M). This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS |
Proposed epitopes | α1-->3 linkages seem to be an important part of the epitope |
IEDB Epitope | N/A |
Proposed Utility | This mAb may be particularly useful for typingsmooth Brucella strains |
Curator ID | AA + AS |
Date of Curation | 19-03-2010 |
References | 1382111 |
PolysacDB ID | 1223 |
Carbohydrate Name | Smooth Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M |
BCSDB Structure | N/A |
Proposed functions | LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 18B04 |
Antibody type and class | IgM |
Assay System | ELISA and immunoblotting |
Cross-reactivity | This antibody was highly specific for S-LPS of the M dominant strain (M). but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS |
Proposed epitopes | α1-->3 linkages seem to be an important part of the epitope |
IEDB Epitope | N/A |
Proposed Utility | This mAb may be particularly useful for typingsmooth Brucella strains |
Curator ID | AA + AS |
Date of Curation | 20-03-2010 |
References | 1382111 |
PolysacDB ID | 1224 |
Carbohydrate Name | Smooth Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M |
BCSDB Structure | N/A |
Proposed functions | LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 04F03 |
Antibody type and class | IgM |
Assay System | ELISA and immunoblotting |
Cross-reactivity | This antibody was highly specific for S-LPS of the M dominant strain (M). but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS |
Proposed epitopes | α1-->3 linkages seem to be an important part of the epitope |
IEDB Epitope | N/A |
Proposed Utility | This mAb may be particularly useful for typingsmooth Brucella strains |
Curator ID | AA + AS |
Date of Curation | 20-03-2010 |
References | 1382111 |
Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020 |