PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

Total Entries - 23
Entry No. - 1 [TOP]

PolysacDB ID	1202
Carbohydrate Name	Smooth Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab12G12
Antibody type and class	N/A
Assay System	ELISA, immunogold stain labelling and immunoblotting
Cross-reactivity	This Mab reacted with smooth Brucellu abortus strain 99 and the smooth B. melitensis strain 16M
Proposed epitopes	This Mab was proposed to be directed to antigenic determinants present along the length of the polymeric O-chain
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	06-03-2010
References	1382111

Entry No. - 2 [TOP]

PolysacDB ID	1203
Carbohydrate Name	Rough Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab A68
Antibody type and class	N/A
Assay System	ELISA, immunogold stain labelling and immunoblotting
Cross-reactivity	This antibody was specific to R-LPS of B. melitensis
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	06-03-2010
References	1382111

Entry No. - 3 [TOP]

PolysacDB ID	1204
Carbohydrate Name	Smooth Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M
BCSDB Structure	N/A
Proposed functions	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 05D01
Antibody type and class	IgG1
Assay System	ELISA and immunoblotting
Cross-reactivity	This antibody was specific to a common epitope on A and M polysaccharides. This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS
Proposed epitopes	This Mab was proposed to be directed to antigenic determinants present along the length of the polymeric O-chain
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	07-03-2010
References	1382111

Entry No. - 4 [TOP]

PolysacDB ID	1205
Carbohydrate Name	Smooth Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M
BCSDB Structure	N/A
Proposed functions	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 07F09
Antibody type and class	IgG1
Assay System	ELISA and immunoblotting
Cross-reactivity	This antibody was specific to a common epitope on A and M polysaccharides. This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS
Proposed epitopes	Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope
IEDB Epitope	N/A
Proposed Utility	This mAb may be particularly useful for typingsmooth Brucella strains
Curator ID	AA + AS
Date of Curation	07-03-2010
References	1382111

Entry No. - 5 [TOP]

PolysacDB ID	1206
Carbohydrate Name	Smooth Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M
BCSDB Structure	N/A
Proposed functions	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 13A09
Antibody type and class	IgG1
Assay System	ELISA and immunoblotting
Cross-reactivity	This antibody was specific to a common epitope on A and M polysaccharides. This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS
Proposed epitopes	Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope
IEDB Epitope	N/A
Proposed Utility	This mAb may be particularly useful for typingsmooth Brucella strains
Curator ID	AA + AS
Date of Curation	07-03-2010
References	1382111

Entry No. - 6 [TOP]

PolysacDB ID	1207
Carbohydrate Name	Smooth Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M
BCSDB Structure	N/A
Proposed functions	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 14f12
Antibody type and class	IgG1
Assay System	ELISA and immunoblotting
Cross-reactivity	This antibody was specific to a common epitope on A and M polysaccharides. This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS
Proposed epitopes	Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope
IEDB Epitope	N/A
Proposed Utility	This mAb may be particularly useful for typingsmooth Brucella strains
Curator ID	AA + AS
Date of Curation	08-03-2010
References	1382111

Entry No. - 7 [TOP]

PolysacDB ID	1208
Carbohydrate Name	Smooth Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M
BCSDB Structure	N/A
Proposed functions	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 16F01
Antibody type and class	IgG1
Assay System	ELISA and immunoblotting
Cross-reactivity	This antibody was specific to a common epitope on A and M polysaccharides. This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS
Proposed epitopes	Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope
IEDB Epitope	N/A
Proposed Utility	This mAb may be particularly useful for typingsmooth Brucella strains
Curator ID	AA + AS
Date of Curation	09-03-2010
References	1382111

Entry No. - 8 [TOP]

PolysacDB ID	1209
Carbohydrate Name	Smooth Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M
BCSDB Structure	N/A
Proposed functions	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 13G11
Antibody type and class	IgM
Assay System	ELISA and immunoblotting
Cross-reactivity	This antibody was specific to a common epitope on A and M polysaccharides. This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS
Proposed epitopes	Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope
IEDB Epitope	N/A
Proposed Utility	This mAb may be particularly useful for typingsmooth Brucella strains
Curator ID	AA + AS
Date of Curation	09-03-2010
References	1382111

Entry No. - 9 [TOP]

PolysacDB ID	1210
Carbohydrate Name	Smooth Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M
BCSDB Structure	N/A
Proposed functions	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 05E11
Antibody type and class	IgM
Assay System	ELISA and immunoblotting
Cross-reactivity	This antibody was specific to a common epitope on A and M polysaccharides. This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS
Proposed epitopes	Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope
IEDB Epitope	N/A
Proposed Utility	This mAb may be particularly useful for typingsmooth Brucella strains
Curator ID	AA + AS
Date of Curation	10-03-2010
References	1382111

Entry No. - 10 [TOP]

PolysacDB ID	1211
Carbohydrate Name	Smooth Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M
BCSDB Structure	N/A
Proposed functions	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 14H09
Antibody type and class	IgM
Assay System	ELISA and immunoblotting
Cross-reactivity	This antibody was specific to a common epitope on A and M polysaccharides. This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS
Proposed epitopes	Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope
IEDB Epitope	N/A
Proposed Utility	This mAb may be particularly useful for typingsmooth Brucella strains
Curator ID	AA + AS
Date of Curation	10-03-2010
References	1382111

Entry No. - 11 [TOP]

PolysacDB ID	1212
Carbohydrate Name	Smooth Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M
BCSDB Structure	N/A
Proposed functions	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 15B10
Antibody type and class	IgG2a
Assay System	ELISA and immunoblotting
Cross-reactivity	This antibody was specific to a common epitope on A and M polysaccharides. But was more reactive towards M dominant strain B. melitensis 16M. This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS
Proposed epitopes	Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope
IEDB Epitope	N/A
Proposed Utility	This mAb may be particularly useful for typingsmooth Brucella strains
Curator ID	AA + AS
Date of Curation	11-03-2010
References	1382111

Entry No. - 12 [TOP]

PolysacDB ID	1213
Carbohydrate Name	Smooth Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M
BCSDB Structure	N/A
Proposed functions	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 02B05
Antibody type and class	IgG3
Assay System	ELISA and immunoblotting
Cross-reactivity	This antibody was specific to a common epitope on A and M polysaccharides. But was more reactive towards M dominant strain B. melitensis 16M. This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS
Proposed epitopes	Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope
IEDB Epitope	N/A
Proposed Utility	This mAb may be particularly useful for typingsmooth Brucella strains
Curator ID	AA + AS
Date of Curation	11-03-2010
References	1382111

Entry No. - 13 [TOP]

PolysacDB ID	1214
Carbohydrate Name	Smooth Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M
BCSDB Structure	N/A
Proposed functions	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 03D03
Antibody type and class	IgG3
Assay System	ELISA and immunoblotting
Cross-reactivity	This antibody was specific to a common epitope on A and M polysaccharides. But was more reactive towards M dominant strain B. melitensis 16M.This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS
Proposed epitopes	Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope
IEDB Epitope	N/A
Proposed Utility	This mAb may be particularly useful for typingsmooth Brucella strains
Curator ID	AA + AS
Date of Curation	12-03-2010
References	1382111

Entry No. - 14 [TOP]

PolysacDB ID	1215
Carbohydrate Name	Smooth Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M
BCSDB Structure	N/A
Proposed functions	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 04A05
Antibody type and class	IgG3
Assay System	ELISA and immunoblotting
Cross-reactivity	This antibody was specific to a common epitope on A and M polysaccharides. But was more reactive towards M dominant strain B. melitensis 16M.This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS
Proposed epitopes	Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope
IEDB Epitope	N/A
Proposed Utility	This mAb may be particularly useful for typingsmooth Brucella strains
Curator ID	AA + AS
Date of Curation	12-03-2010
References	1382111

Entry No. - 15 [TOP]

PolysacDB ID	1216
Carbohydrate Name	Smooth Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M
BCSDB Structure	N/A
Proposed functions	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 11H03
Antibody type and class	IgG3
Assay System	ELISA and immunoblotting
Cross-reactivity	This antibody was specific to a common epitope on A and M polysaccharides. But was more reactive towards M dominant strain B. melitensis 16M.This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS
Proposed epitopes	Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope
IEDB Epitope	N/A
Proposed Utility	This mAb may be particularly useful for typingsmooth Brucella strains
Curator ID	AA + AS
Date of Curation	12-03-2010
References	1382111

Entry No. - 16 [TOP]

PolysacDB ID	1217
Carbohydrate Name	Smooth Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M
BCSDB Structure	N/A
Proposed functions	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 12B12
Antibody type and class	IgG3
Assay System	ELISA and immunoblotting
Cross-reactivity	This antibody was specific to a common epitope on A and M polysaccharides. But was more reactive towards M dominant strain B. melitensis 16M.This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS
Proposed epitopes	Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope
IEDB Epitope	N/A
Proposed Utility	This mAb may be particularly useful for typingsmooth Brucella strains
Curator ID	AA + AS
Date of Curation	19-03-2010
References	1382111

Entry No. - 17 [TOP]

PolysacDB ID	1218
Carbohydrate Name	Smooth Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M
BCSDB Structure	N/A
Proposed functions	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 17H02
Antibody type and class	IgG3
Assay System	ELISA and immunoblotting
Cross-reactivity	This antibody was specific to a common epitope on A and M polysaccharides. But was more reactive towards M dominant strain B. melitensis 16M.This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS
Proposed epitopes	Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope
IEDB Epitope	N/A
Proposed Utility	This mAb may be particularly useful for typingsmooth Brucella strains
Curator ID	AA + AS
Date of Curation	19-03-2010
References	1382111

Entry No. - 18 [TOP]

PolysacDB ID	1219
Carbohydrate Name	Smooth Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M
BCSDB Structure	N/A
Proposed functions	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 01F08
Antibody type and class	IgG3
Assay System	ELISA and immunoblotting
Cross-reactivity	This antibody was specific to a common epitope on A and M polysaccharides. But was more reactive towards M dominant strain B. melitensis 16M.This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS
Proposed epitopes	Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope
IEDB Epitope	N/A
Proposed Utility	This mAb may be particularly useful for typingsmooth Brucella strains
Curator ID	AA + AS
Date of Curation	19-03-2010
References	1382111

Entry No. - 19 [TOP]

PolysacDB ID	1220
Carbohydrate Name	Smooth Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M
BCSDB Structure	N/A
Proposed functions	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 08C06
Antibody type and class	IgG3
Assay System	ELISA and immunoblotting
Cross-reactivity	This antibody was specific to a common epitope on A and M polysaccharides. But was more reactive towards M dominant strain B. melitensis 16M.This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS
Proposed epitopes	Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope
IEDB Epitope	N/A
Proposed Utility	This mAb may be particularly useful for typingsmooth Brucella strains
Curator ID	AA + AS
Date of Curation	19-03-2010
References	1382111

Entry No. - 20 [TOP]

PolysacDB ID	1221
Carbohydrate Name	Smooth Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M
BCSDB Structure	N/A
Proposed functions	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 08B07
Antibody type and class	IgG3
Assay System	ELISA and immunoblotting
Cross-reactivity	This antibody was specific to a common epitope on A and M polysaccharides. But was more reactive towards M dominant strain B. melitensis 16M.This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS
Proposed epitopes	Both α1-->2 and α1-->3 linkages seem to be an important part of the epitope
IEDB Epitope	N/A
Proposed Utility	This mAb may be particularly useful for typingsmooth Brucella strains
Curator ID	AA + AS
Date of Curation	19-03-2010
References	1382111

Entry No. - 21 [TOP]

PolysacDB ID	1222
Carbohydrate Name	Smooth Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M
BCSDB Structure	N/A
Proposed functions	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 03E05
Antibody type and class	IgG3
Assay System	ELISA and immunoblotting
Cross-reactivity	This antibody was highly specific for S-LPS of the M dominant strain (M). This Mab cross-reacted with O-PS of B. abortus 99 but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS
Proposed epitopes	α1-->3 linkages seem to be an important part of the epitope
IEDB Epitope	N/A
Proposed Utility	This mAb may be particularly useful for typingsmooth Brucella strains
Curator ID	AA + AS
Date of Curation	19-03-2010
References	1382111

Entry No. - 22 [TOP]

PolysacDB ID	1223
Carbohydrate Name	Smooth Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M
BCSDB Structure	N/A
Proposed functions	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 18B04
Antibody type and class	IgM
Assay System	ELISA and immunoblotting
Cross-reactivity	This antibody was highly specific for S-LPS of the M dominant strain (M). but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS
Proposed epitopes	α1-->3 linkages seem to be an important part of the epitope
IEDB Epitope	N/A
Proposed Utility	This mAb may be particularly useful for typingsmooth Brucella strains
Curator ID	AA + AS
Date of Curation	20-03-2010
References	1382111

Entry No. - 23 [TOP]

PolysacDB ID	1224
Carbohydrate Name	Smooth Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella melitensis strain B115 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The S-LPS of smooth-type Brucella spp. contains two distinct antigenic determinants designated A and M
BCSDB Structure	N/A
Proposed functions	LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 04F03
Antibody type and class	IgM
Assay System	ELISA and immunoblotting
Cross-reactivity	This antibody was highly specific for S-LPS of the M dominant strain (M). but did not bind to R-LPS of B. melitensis B11; also did not cross-react with Y. enterocolitica0:9 LPS
Proposed epitopes	α1-->3 linkages seem to be an important part of the epitope
IEDB Epitope	N/A
Proposed Utility	This mAb may be particularly useful for typingsmooth Brucella strains
Curator ID	AA + AS
Date of Curation	20-03-2010
References	1382111

Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020