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| PolysacDB ID | 1243 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Brucella melitensis 16M (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | O-polysaccharide is a homopolymer of 4,6-dideoxy-4-formamidoct-D-mannopyranose residues |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | B.melitensis 16M formaldehyde-killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab BM2-10 |
| Antibody type and class | IgG3 |
| Assay System | ELISA, immunodiffusion and competitive inhibition assay |
| Cross-reactivity | This antibody cross-reacted with the O-polysaccharide of B. abortus and did not cross-react with the Yersinia enterocolitica O:9 polysaccharide |
| Proposed epitopes | This Mab reacted with both A and M antigens. The crucial element of the M epitope is a disaccharide, α-D-Rha4NFo(1-->3)α-D Rha4NFo, that requires adjacent αl,2-linked residues for full activity. The common epitope of A and M antigens is a linear α1,2-linked tri or tetrasaccharide |
| IEDB Epitope | 76740 |
| Proposed Utility | This Mab can be used as a use as standard Brucella typing or detection reagents |
| Curator ID | AA + AS |
| Date of Curation | 04-04-2010 |
| References | PMC313534 |
| PolysacDB ID | 1244 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Brucella melitensis 16M (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | O-polysaccharide is a homopolymer of 4,6-dideoxy-4-formamidoct-D-mannopyranose residues |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | B.melitensis 16M formaldehyde-killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab BM2-15 |
| Antibody type and class | IgG3 |
| Assay System | ELISA, immunodiffusion and competitive inhibition assay |
| Cross-reactivity | This antibody was specific to B. melitiensis and did not cross-reac with O-polysaccharide of B. abortus and Yersinia enterocolitica O:9 polysaccharide |
| Proposed epitopes | This Mab reacted only with M antigen. The A epitope is a pentasaccharide or larger oligosaccharide composed of α1,2-linked 4,6-dideoxy-4-formamido-D-mannopyranosyl (D-Rha4NFo) residues in which the formamido residue plays a crucial role The crucial element of the M epitope is a disaccharide, α-D-Rha4NFo(1-->3)α-D Rha4NFo, that requires adjacent α1,2-linked residues for full activity. The common epitope of A and M antigens is a linear α1,2-linked tri or tetrasaccharide |
| IEDB Epitope | 76740 |
| Proposed Utility | This Mab can be used as a use as standard Brucella typing or detection reagents |
| Curator ID | AA + AS |
| Date of Curation | 05-04-2010 |
| References | PMC313534 |
| PolysacDB ID | 1245 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Brucella melitensis 16M (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | O-polysaccharide is a homopolymer of 4,6-dideoxy-4-formamidoct-D-mannopyranose residues |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | B.melitensis 16M formaldehyde-killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab BM2-28 |
| Antibody type and class | IgG3 |
| Assay System | ELISA, immunodiffusion and competitive inhibition assay |
| Cross-reactivity | This antibody was specific to B. melitiensis and did not cross-reac with O-polysaccharide of B. abortus and Yersinia enterocolitica O:9 polysaccharide |
| Proposed epitopes | This Mab reacted only with M antigen. The crucial element of the M epitope is a disaccharide, α-D-Rha4NFo(1-->3)α-D Rha4NFo, that requires adjacent α1,2-linked residues for full activity. The common epitope of A and M antigens is a linear α1,2-linked tri or tetrasaccharide |
| IEDB Epitope | 76740 |
| Proposed Utility | This Mab can be used as a use as standard Brucella typing or detection reagents |
| Curator ID | AA + AS |
| Date of Curation | 05-04-2010 |
| References | PMC313534 |
| PolysacDB ID | 1246 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Brucella melitensis 16M (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | O-polysaccharide is a homopolymer of 4,6-dideoxy-4-formamidoct-D-mannopyranose residues |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | B.melitensis 16M formaldehyde-killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab BM3-2 |
| Antibody type and class | IgG3 |
| Assay System | ELISA, immunodiffusion and competitive inhibition assay |
| Cross-reactivity | This antibody was specific to B. melitiensis and did not cross-reac with O-polysaccharide of B. abortus and Yersinia enterocolitica O:9 polysaccharide |
| Proposed epitopes | This Mab reacted only with M antigen. The crucial element of the M epitope is a disaccharide, α-D-Rha4NFo(1-->3)α-D Rha4NFo, that requires adjacent α1,2-linked residues for full activity. The common epitope of A and M antigens is a linear α1,2-linked tri or tetrasaccharide |
| IEDB Epitope | 76740 |
| Proposed Utility | This Mab can be used as a use as standard Brucella typing or detection reagents |
| Curator ID | AA + AS |
| Date of Curation | 05-04-2010 |
| References | PMC313534 |
| PolysacDB ID | 1247 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Brucella melitensis 16M (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | O-polysaccharide is a homopolymer of 4,6-dideoxy-4-formamidoct-D-mannopyranose residues |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | B.melitensis 16M formaldehyde-killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab BM3-7 |
| Antibody type and class | IgG2b |
| Assay System | ELISA, immunodiffusion and competitive inhibition assay |
| Cross-reactivity | This antibody was specific to B. melitiensis and did not cross-reac with O-polysaccharide of B. abortus and Yersinia enterocolitica O:9 polysaccharide |
| Proposed epitopes | This Mab reacted only with M antigen. The crucial element of the M epitope is a disaccharide, α-D-Rha4NFo(1-->3)α-D Rha4NFo, that requires adjacent α1,2-linked residues for full activity. The common epitope of A and M antigens is a linear α1,2-linked tri or tetrasaccharide |
| IEDB Epitope | 76740 |
| Proposed Utility | This Mab can be used as a use as standard Brucella typing or detection reagents |
| Curator ID | AA + AS |
| Date of Curation | 07-04-2010 |
| References | PMC313534 |
| PolysacDB ID | 1248 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Brucella melitensis 16M (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | O-polysaccharide is a homopolymer of 4,6-dideoxy-4-formamidoct-D-mannopyranose residues |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | B.melitensis 16M formaldehyde-killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab BM3-8 |
| Antibody type and class | IgG2a |
| Assay System | ELISA, immunodiffusion and competitive inhibition assay |
| Cross-reactivity | This antibody was specific to B. melitiensis and did not cross-reac with O-polysaccharide of B. abortus and Yersinia enterocolitica O:9 polysaccharide |
| Proposed epitopes | This Mab reacted only with M antigen. The crucial element of the M epitope is a disaccharide, α-D-Rha4NFo(1-->3)α-D Rha4NFo, that requires adjacent α1,2-linked residues for full activity. The common epitope of A and M antigens is a linear α1,2-linked tri or tetrasaccharide |
| IEDB Epitope | 76740 |
| Proposed Utility | This Mab can be used as a use as standard Brucella typing or detection reagents |
| Curator ID | AA + AS |
| Date of Curation | 07-04-2010 |
| References | PMC313534 |
| PolysacDB ID | 1249 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Brucella melitensis 16M (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Brucella LPS-PS complexes consist of the Brucella O-chains, native hapten, and polysaccharide B |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | B.melitensis 16M whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab M |
| Antibody type and class | N/A |
| Assay System | Immunoblotting, ELISA, immunoaffinity chromatography, immunoprecipitation, |
| Cross-reactivity | This antibody was specific to Brucella melitiensis 16M strain and did not cross-react with Brucella abortus 2308, Brucella abortus 19 and Brucella abortus type 4 strains |
| Proposed epitopes | This mab was specific to the M epitope |
| IEDB Epitope | 76690 |
| Proposed Utility | This Mab would provide a tool to monitor the expression of LPS epitopes by Brucella spp. and determine the content of preparations intended for use in the control of brucellosis |
| Curator ID | AA + AS |
| Date of Curation | 08-04-2010 |
| References | PMC267019 |
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Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020 |