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| PolysacDB ID | 1239 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Brucella abortus W99 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | O-polysaccharide consists of an unbranched linear homopolymer of 10 to 100 α-1,2-linked 4-formamido-4,6-dideoxy-D-mannose (N formylperosamine) residues. The O-polysaccharide is covalently linked to a core oligosaccharide |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Whole B. abortus W99 cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab 4F9 |
| Antibody type and class | IgG3 |
| Assay System | ELISA |
| Cross-reactivity | This antibody did not cross-react with S-LPSs of S. urbana, Vibrio cholerae and E. coli O:157, but cross-reacted with S-LPS of Y. enterocolitica O:9 along with Brucella A and M dominant strains. Reactivity was more towards A dominant strains |
| Proposed epitopes | This Mab was directed against the C/Y epitope and did not consist of α1,2-linked D-Rha4NFo residues. This Mab did not bind purified O-polysaccharide |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab helped in the epitope strucure elucidation |
| Curator ID | AA + AS |
| Date of Curation | 03-04-2010 |
| References | PMC175246 |
| PolysacDB ID | 1242 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Brucella abortus W99 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | O-polysaccharide consists of an unbranched linear homopolymer of 10 to 100 α-1,2-linked 4-formamido-4,6-dideoxy-D-mannose (N formylperosamine) residues. The O-polysaccharide is covalently linked to a core oligosaccharide |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Whole B. abortus W99 cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab 2C8C4 |
| Antibody type and class | IgG3 |
| Assay System | ELISA |
| Cross-reactivity | This Mab was specific to A strains of Brucella and did not cross-react with Brucella M strain, Y enterocolitica O:9, S-LPSs of S.urbana, Vibrio cholerae and E. coli O:157 |
| Proposed epitopes | The epitope was proposed to be a pentasaccharide or larger oligosaccharide composed of α1,2-linked D-Rha4NFo and also contained α1,3 linkages |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab helped in the epitope strucure elucidation |
| Curator ID | AA + AS |
| Date of Curation | 04-04-2010 |
| References | PMC175246 |
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Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020 |