PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

Total Entries - 3
Entry No. - 1 [TOP]

PolysacDB ID	1225
Carbohydrate Name	O-polysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella abortus 2308 (NCBI Taxonomy) (Drugpedia)
Basic Structure	Brucella O-PS structure has been defined as homopolymers of 4,6-dideoxy-4-formamido-α-D-mannopyranose residues
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Irradiated cells of B. abortus smooth strain 2308
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 34
Antibody type and class	N/A
Assay System	ELISA, invivo protection studies
Cross-reactivity	N/A
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab confers protection in mice against experimental brucellosis
Curator ID	AA + AS
Date of Curation	23-03-2010
References	PMC260994

Entry No. - 2 [TOP]

PolysacDB ID	1226
Carbohydrate Name	O-polysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella abortus 2308 (NCBI Taxonomy) (Drugpedia)
Basic Structure	Brucella O-PS structure has been defined as homopolymers of 4,6-dideoxy-4-formamido-α-D-mannopyranose residues
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Irradiated cells of B. abortus smooth strain 2309
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 47
Antibody type and class	N/A
Assay System	ELISA, invivo protection studies
Cross-reactivity	N/A
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab confers protection in mice against experimental brucellosis
Curator ID	AA + AS
Date of Curation	23-03-2010
References	PMC260994

Entry No. - 3 [TOP]

PolysacDB ID	1250
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella abortus 2308 (NCBI Taxonomy) (Drugpedia)
Basic Structure	Brucella LPS-PS complexes consist of the Brucella O-chains, native hapten, and polysaccharide B
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole Brucella. Abortus 2308 cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab A
Antibody type and class	N/A
Assay System	Immunoblotting, ELISA, immunoaffinity chromatography, immunoprecipitation,
Cross-reactivity	This antibody was specific to Brucella abortus 2308 strain and did not cross-react with Brucella melitiensis 16M strain and Brucella abortus type 4 strains. It reacted with Brucella abortus 19 strain
Proposed epitopes	This mab was specific to the A epitope
IEDB Epitope	N/A
Proposed Utility	This Mab would provide a tool to monitor the expression of LPS epitopes by Brucella spp. and determine the content of preparations intended for use in the control of brucellosis
Curator ID	AA + AS
Date of Curation	08-04-2010
References	PMC267019

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