PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

Total Entries - 10
Entry No. - 1 [TOP]

PolysacDB ID	1192
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella abortus 1119-3 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Killed whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	B.ab.-1
Antibody type and class	IgM
Assay System	Whole cell agglutination, immunodiffusion, immunoblotting and ELISA
Cross-reactivity	This Mab agglutinated Yersinia and Brucella abortus cells and had high ELISA titers, but which failed to precipitate the polysaccharide antigens
Proposed epitopes	The epitope was proposed to be Terminal nonreducing portions of the O-chain
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to study the cell wall polysaccharide antigens of other Y. enterocolitica strains and are being evaluated for specific serological identification of B. abortus.
Curator ID	AA + AS
Date of Curation	03-03-2010
References	PMC261544

Entry No. - 2 [TOP]

PolysacDB ID	1193
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella abortus 1119-3 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Killed whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	B.ab.-2
Antibody type and class	IgG1
Assay System	Whole cell agglutination, immunodiffusion, immunoblotting and ELISA
Cross-reactivity	This Mab agglutinated Yersinia and Brucella abortus cells and had high ELISA titers, but which failed to precipitate the polysaccharide antigens
Proposed epitopes	The epitope was proposed to be Terminal nonreducing portions of the O-chain
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to study the cell wall polysaccharide antigens of other Y. enterocolitica strains and are being evaluated for specific serological identification of B. abortus.
Curator ID	AA + AS
Date of Curation	03-03-2010
References	PMC261544

Entry No. - 3 [TOP]

PolysacDB ID	1194
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella abortus 1119-3 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Killed whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	B.ab.-3
Antibody type and class	IgG3
Assay System	Whole cell agglutination, immunodiffusion, immunoblotting and ELISA
Cross-reactivity	This Mab agglutinated Yersinia and Brucella abortus cells and had high ELISA titers, but which failed to precipitate the polysaccharide antigens
Proposed epitopes	The epitope was proposed to be Terminal nonreducing portions of the O-chain
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to study the cell wall polysaccharide antigens of other Y. enterocolitica strains and are being evaluated for specific serological identification of B. abortus.
Curator ID	AA + AS
Date of Curation	04-03-2010
References	PMC261544

Entry No. - 4 [TOP]

PolysacDB ID	1195
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella abortus 1119-3 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Killed whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	B.ab.-4
Antibody type and class	IgG3
Assay System	Whole cell agglutination, immunodiffusion, immunoblotting and ELISA
Cross-reactivity	This Mab cross-reacted with LPS and 0-chains of Y. enterocolitica and B. abortus and exhibited high ELISA titers with homologous and heterologous antigens, in addition to agglutinating activity for both bacteria
Proposed epitopes	This Mab was proposed to be directed to antigenic determinants present along the length of the polymeric O-chain
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to study the cell wall polysaccharide antigens of other Y. enterocolitica strains and are being evaluated for specific serological identification of B. abortus.
Curator ID	AA + AS
Date of Curation	04-03-2010
References	PMC261544

Entry No. - 5 [TOP]

PolysacDB ID	1196
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella abortus 1119-3 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Killed whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	B.ab.-5
Antibody type and class	IgG1
Assay System	Whole cell agglutination, immunodiffusion, immunoblotting and ELISA
Cross-reactivity	This Mab agglutinated Yersinia and Brucella abortus cells and had high ELISA titers, but which failed to precipitate the polysaccharide antigens
Proposed epitopes	The epitope was proposed to be Terminal nonreducing portions of the O-chain
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to study the cell wall polysaccharide antigens of other Y. enterocolitica strains and are being evaluated for specific serological identification of B. abortus.
Curator ID	AA + AS
Date of Curation	04-03-2010
References	PMC261544

Entry No. - 6 [TOP]

PolysacDB ID	1197
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella abortus 1119-3 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Killed whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	B.ab.-6
Antibody type and class	IgM
Assay System	Whole cell agglutination, immunodiffusion, immunoblotting and ELISA
Cross-reactivity	This Mab cross-reacted with LPS and 0-chains of Y. enterocolitica and B. abortus and exhibited high ELISA titers with homologous and heterologous antigens, in addition to agglutinating activity for both bacteria
Proposed epitopes	This Mab was proposed to be directed to antigenic determinants present along the length of the polymeric O-chain
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to study the cell wall polysaccharide antigens of other Y. enterocolitica strains and are being evaluated for specific serological identification of B. abortus.
Curator ID	AA + AS
Date of Curation	05-03-2010
References	PMC261544

Entry No. - 7 [TOP]

PolysacDB ID	1198
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella abortus 1119-3 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Killed whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	B.ab.-7
Antibody type and class	IgG3
Assay System	Whole cell agglutination, immunodiffusion, immunoblotting and ELISA
Cross-reactivity	This Mab cross-reacted with LPS and 0-chains of Y. enterocolitica and B. abortus and exhibited high ELISA titers with homologous and heterologous antigens, in addition to agglutinating activity for both bacteria
Proposed epitopes	This Mab was proposed to be directed to antigenic determinants present along the length of the polymeric O-chain
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to study the cell wall polysaccharide antigens of other Y. enterocolitica strains and are being evaluated for specific serological identification of B. abortus.
Curator ID	AA + AS
Date of Curation	05-03-2010
References	PMC261544

Entry No. - 8 [TOP]

PolysacDB ID	1199
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella abortus 1119-3 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Killed whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	B.ab.-8
Antibody type and class	IgG3
Assay System	Whole cell agglutination, immunodiffusion, immunoblotting and ELISA
Cross-reactivity	This Mab cross-reacted with LPS and 0-chains of Y. enterocolitica and B. abortus and exhibited high ELISA titers with homologous and heterologous antigens, in addition to agglutinating activity for both bacteria
Proposed epitopes	This Mab was proposed to be directed to antigenic determinants present along the length of the polymeric O-chain
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to study the cell wall polysaccharide antigens of other Y. enterocolitica strains and are being evaluated for specific serological identification of B. abortus.
Curator ID	AA + AS
Date of Curation	05-03-2010
References	PMC261544

Entry No. - 9 [TOP]

PolysacDB ID	1200
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella abortus 1119-3 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Killed whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	B.ab.-9
Antibody type and class	IgM
Assay System	Whole cell agglutination, immunodiffusion, immunoblotting and ELISA
Cross-reactivity	This Mab agglutinated Yersinia and Brucella abortus cells and had high ELISA titers, but which failed to precipitate the polysaccharide antigens
Proposed epitopes	The epitope was proposed to be Terminal nonreducing portions of the O-chain
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to study the cell wall polysaccharide antigens of other Y. enterocolitica strains and are being evaluated for specific serological identification of B. abortus.
Curator ID	AA + AS
Date of Curation	05-03-2010
References	PMC261544

Entry No. - 10 [TOP]

PolysacDB ID	1201
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Brucella abortus 1119-3 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Killed whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	B.ab.-10
Antibody type and class	IgG3
Assay System	Whole cell agglutination, immunodiffusion, immunoblotting and ELISA
Cross-reactivity	This Mab cross-reacted with LPS and 0-chains of Y. enterocolitica and B. abortus and exhibited high ELISA titers with homologous and heterologous antigens, in addition to agglutinating activity for both bacteria
Proposed epitopes	This Mab was proposed to be directed to antigenic determinants present along the length of the polymeric O-chain
IEDB Epitope	N/A
Proposed Utility	This Mab can be used to study the cell wall polysaccharide antigens of other Y. enterocolitica strains and are being evaluated for specific serological identification of B. abortus.
Curator ID	AA + AS
Date of Curation	06-03-2010
References	PMC261544

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