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| PolysacDB ID | 1164 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Bordetella pertusis 1414 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | B. pertusis LPS is resolved electrophoretically into two bands, a slowly migrating band designated LOS A and a fast-migrating band designated LOS B. These two species differ in molecular weight as three N-acetyl amino sugars found on LOS A are not present on LOS B |
| BCSDB Structure | N/A |
| Proposed functions | The LOS of B. pertussis exhibits biological activities similar to those of endotoxins from other gram-negative bacteria, including pyrogenicity, toxicity, induction of the Shwartzman reaction, induction of tumor necrosis factor production, and adjuvant properties |
| Antigenic Nature used to produce antibodies | B. pertusis LPS |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab P1P3 |
| Antibody type and class | IgM |
| Assay System | ELISA and Immunostaining |
| Cross-reactivity | This Mab did not cross-react with the B. pertusis variant strain A100 but cross-reacted with a B. bronchiseptica LPS and B. parapertussis LPS |
| Proposed epitopes | The epitope was proposed to be terminal sugar units nonacetylated glucosamine substituted with L-glycero-b-manno-heptose, present in the 'core' of the B. pertussis LPS. The carbon atoms 3,4, and 6 of this glucosamine unit could be involved in the epitope |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab can be useful for rapid typing of Bordetella in clinical isolates and can be used to detect antigenic modifications in LPSs from variant B. pertassis strains, or from related Bordetella species (B. parapertassis, B. bronchiseptica |
| Curator ID | AA + AS |
| Date of Curation | 24-02-2010 |
| References | 8936324 |
| PolysacDB ID | 1165 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Bordetella pertusis 1414 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | B. pertusis LPS is resolved electrophoretically into two bands, a slowly migrating band designated LOS A and a fast-migrating band designated LOS B. These two species differ in molecular weight as three N-acetyl amino sugars found on LOS A are not present on LOS B |
| BCSDB Structure | N/A |
| Proposed functions | The LOS of B. pertussis exhibits biological activities similar to those of endotoxins from other gram-negative bacteria, including pyrogenicity, toxicity, induction of the Shwartzman reaction, induction of tumor necrosis factor production, and adjuvant properties |
| Antigenic Nature used to produce antibodies | B. pertusis LPS |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab D7 |
| Antibody type and class | IgG3 |
| Assay System | ELISA and Immunostaining |
| Cross-reactivity | Did not cross-react with the B. pertusis variant strain A101 |
| Proposed epitopes | The epitope recognized by D7 is affected by the addition of the O-side chain and that a portion of the distal trisaccharide could be involved in this epitope. This suggests therefore that this epitope may consist of the FucN(Ac)Me-->GlcN disaccharide |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab can be useful for rapid typing of Bordetella in clinical isolates and can be used to detect antigenic modifications in LPSs from variant B. pertassis strains, or from related Bordetella species (B. parapertassis, B. bronchiseptica |
| Curator ID | AA + AS |
| Date of Curation | 24-02-2010 |
| References | 8936324 |
| PolysacDB ID | 1166 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Bordetella pertusis 1414 (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | B. pertusis LPS is resolved electrophoretically into two bands, a slowly migrating band designated LOS A and a fast-migrating band designated LOS B. These two species differ in molecular weight as three N-acetyl amino sugars found on LOS A are not present on LOS B |
| BCSDB Structure | N/A |
| Proposed functions | The LOS of B. pertussis exhibits biological activities similar to those of endotoxins from other gram-negative bacteria, including pyrogenicity, toxicity, induction of the Shwartzman reaction, induction of tumor necrosis factor production, and adjuvant properties |
| Antigenic Nature used to produce antibodies | B. pertusis Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab 60.5 |
| Antibody type and class | IgM |
| Assay System | ELISA and Immunostaining |
| Cross-reactivity | This Mab did not cross-react with the B. pertusis variant strain A102 but cross-reacted with a B. bronchiseptica LPS |
| Proposed epitopes | The epitope was proposed to be terminal region of a distal trisaccharide GlcNAc-->2,3-NAcManA-->FucN(Ac)Me consisting of Nacetylated amino sugars. A next carbohydrate unit (2,3-NAcManA) may be partially involved in the recognized epitope |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab can be useful for rapid typing of Bordetella in clinical isolates and can be used to detect antigenic modifications in LPSs from variant B. pertassis strains, or from related Bordetella species (B. parapertassis, B. bronchiseptica |
| Curator ID | AA + AS |
| Date of Curation | 25-02-2010 |
| References | 8936324 |
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Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020 |