PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

Total Entries - 6
Entry No. - 1 [TOP]

PolysacDB ID	1037
Carbohydrate Name	Galactomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Aspergillus fumigatus (NCBI Taxonomy) (Drugpedia)
Basic Structure	Galactomannan is composed of a branched core containing α(1-->2)- and α(1-->6)-linked mannose, with β(1-->5)galactofuranose and/or β(1-->4)galactopyranose moieties linked linearly in side chains terminated by galactofuranose nonreducing end units
BCSDB Structure	N/A
Proposed functions	Galactomannan is a major cell wall component in Aspergillusspecies and can also be secreted into the medium as a component of the exoantigen. The galactomannan is immunogenic and polyclonal antibodies against it have been used in antigen detection for the diagnosis of invasive aspergillosis, and in the detection of fungal contamination in foodstuffs
Antigenic Nature used to produce antibodies	Formalinized spores of A. fumigatus.
Carrier Name	N/A
Conjugation Method	N/A
Antibodies	Mab EB-A2
Antibody type and class	IgM
Assay System	Indirect and Competitive ELISA
Cross-reactivity	This antibody cross-reacted to P. digitatum and Penicillium marneffei speices.
Proposed epitopes	3(1-->5)galactofuranose residues of galactomannan
IEDB Epitope	N/A
Proposed Utility	EB-A2 has been used to detect circulating antigen in the serum of patients with invasive aspergillosis by ELISA inhibition. The sensitivity (95%), specificity (100%), positive predictive value (100%), and negative predictive value (99%)of the ELISA
Curator ID	AA + AS
Date of Curation	10-01-2010
References	PMC257149

Entry No. - 2 [TOP]

PolysacDB ID	1043
Carbohydrate Name	Galactomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Aspergillus fumigatus (NCBI Taxonomy) (Drugpedia)
Basic Structure	Galactomannan is composed of a branched core containing α(1-->2)- and α(1-->6)-linked mannose, with β(1-->5)galactofuranose and/or β(1-->4)galactopyranose moieties linked linearly in side chains terminated by galactofuranose nonreducing end units
BCSDB Structure	N/A
Proposed functions	Galactomannan is a major cell wall component in Aspergillusspecies and can also be secreted into the medium as a component of the exoantigen. The galactomannan is immunogenic and polyclonal antibodies against it have been used in antigen detection for the diagnosis of invasive aspergillosis, and in the detection of fungal contamination in foodstuffs
Antigenic Nature used to produce antibodies	Cold alkali extract of mycelium
Carrier Name	N/A
Conjugation Method	N/A
Antibodies	Mab 40
Antibody type and class	IgM
Assay System	Indirect Enzyme Immunoassay, immunofluorescence, Preciptin assay
Cross-reactivity	This antibody was cross-reactive with C.albicans
Proposed epitopes	Mannopyranosyl side chain
IEDB Epitope	N/A
Proposed Utility	This antibody can be used to detect circulating antigen in the serum of patients with invasive aspergillosis by ELISA inhibition.
Curator ID	AA + AS
Date of Curation	13-01-2010
References	PMC258783

Entry No. - 3 [TOP]

PolysacDB ID	1044
Carbohydrate Name	Galactomannan (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Aspergillus fumigatus (NCBI Taxonomy) (Drugpedia)
Basic Structure	Galactomannan is composed of a branched core containing α(1-->2)- and α(1-->6)-linked mannose, with β(1-->5)galactofuranose and/or β(1-->4)galactopyranose moieties linked linearly in side chains terminated by galactofuranose nonreducing end units
BCSDB Structure	N/A
Proposed functions	Galactomannan is a major cell wall component in Aspergillusspecies and can also be secreted into the medium as a component of the exoantigen. The galactomannan is immunogenic and polyclonal antibodies against it have been used in antigen detection for the diagnosis of invasive aspergillosis, and in the detection of fungal contamination in foodstuffs
Antigenic Nature used to produce antibodies	Cold alkali extract of mycelium
Carrier Name	N/A
Conjugation Method	N/A
Antibodies	Mab 1
Antibody type and class	IgM
Assay System	Indirect Enzyme Immunoassay, immunofluorescence, Preciptin assay
Cross-reactivity	This antibody was cross-reactive with C.albicans
Proposed epitopes	Oligogalactoside side chain
IEDB Epitope	N/A
Proposed Utility	This antibody can be used to detect circulating antigen in the serum of patients with invasive aspergillosis by ELISA inhibition.
Curator ID	AA + AS
Date of Curation	13-01-2010
References	PMC258783

Entry No. - 4 [TOP]

PolysacDB ID	1139
Carbohydrate Name	58 Kda antigen (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Aspergillus fumigatus (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	A. fumigatus mycelial extract
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 2-2-1
Antibody type and class	IgG1
Assay System	Immunoblotting, immunoprecipitation, immunodiffusion
Cross-reactivity	This antibody cross-reacted with a 55 kDa antigen Candida albicans extract
Proposed epitopes	The epitope was proposed to consist of mannan residues
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	14-02-2010
References	PMC257743

Entry No. - 5 [TOP]

PolysacDB ID	1140
Carbohydrate Name	58 Kda antigen (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Aspergillus fumigatus (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	A. fumigatus mycelial extract
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 2-2-4
Antibody type and class	IgG1
Assay System	Immunoblotting, immunoprecipitation, immunodiffusion
Cross-reactivity	This antibody cross-reacted with a 55 kDa antigen Candida albicans extract
Proposed epitopes	The epitope was proposed to consist of mannan residues
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	14-02-2010
References	PMC257743

Entry No. - 6 [TOP]

PolysacDB ID	1141
Carbohydrate Name	58 Kda antigen (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Aspergillus fumigatus (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	A. fumigatus mycelial extract
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Mab 2-2-6
Antibody type and class	IgG1
Assay System	Immunoblotting, immunoprecipitation, immunodiffusion
Cross-reactivity	This antibody cross-reacted with a 55 kDa antigen Candida albicans extract
Proposed epitopes	The epitope was proposed to consist of mannan residues
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	14-02-2010
References	PMC257743

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