PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 1

Entry No. - 1   [TOP]
PolysacDB ID1123
Carbohydrate NameO-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeActinobacillus pleuropneumoniae serotype 9   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe structure consists of trisaccharide backbone randomly substituted by N-acetyl-β-D-glucosamine end groups (to the extent of 25%)
BCSDB Structure131785
Proposed functionsLPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus
Antigenic Nature used to produce antibodiesFormalin-fixed A. pleuropneumoniae serotype 9 cells
Carrier NameNil
Conjugation MethodNil
AntibodiesPolysera
Antibody type and classN/A
Assay SystemELISA
Cross-reactivityThis antibody cross-reacted with serotypes 1 and 12. also cross-reacted with deaminated Serotype1 O-PS
Proposed epitopesThe epitope was deduced to contain the terminal nonreducing -D-Glc NAc residues. A second epitope was said to be directed to linear regions of the backbone polymer
IEDB EpitopeN/A
Proposed UtilityThis antisera not only helped in serotyping analysis but also helped in deducing the epitopes recognized
Curator IDAA + AS
Date of Curation06-02-2010
ReferencesPMC206369


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