PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 3

Entry No. - 1   [TOP]
PolysacDB ID1501
Carbohydrate NameO-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeAcinetobacter baumannii strain 24   (NCBI Taxonomy)   (Drugpedia)
Basic StructureAcinetobacter LPSs consist of a polysaccharide covalently linked to a lipid component, termed lipid A, which anchors the LPS in the outer membrane. This polysaccharide is divided into the core oligosaccharide (linked to lipid A) and the O-polysaccharide or O-antigen. This type of LPS is referred to as the smooth- or S-form phenotype; the O-antigens are characteristic for a given LPS and the parental bacterial strain
BCSDB Structure5534
Proposed functionsSince all recently investigated LPSs from Acinetobacter strains have been shown to be of the smooth phenotype, a serotyping scheme for identification of members of this genus may also be possible
Antigenic Nature used to produce antibodiesHeat killed bacteria
Carrier NameNil
Conjugation MethodNil
AntibodiesMab S48-3-13
Antibody type and classIgG3
Assay SystemDot blot and Enzyme Immunoassay
Cross-reactivityThis antibody cross-reacted with 31 strains A. baumannii
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis antibody can be used for strain identification in A. baumanni species
Curator IDAA + AS
Date of Curation05-08-2010
ReferencesPMC103717


Entry No. - 2   [TOP]
PolysacDB ID1502
Carbohydrate NameO-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeAcinetobacter baumannii strain 24   (NCBI Taxonomy)   (Drugpedia)
Basic StructureAcinetobacter LPSs consist of a polysaccharide covalently linked to a lipid component, termed lipid A, which anchors the LPS in the outer membrane. This polysaccharide is divided into the core oligosaccharide (linked to lipid A) and the O-polysaccharide or O-antigen. This type of LPS is referred to as the smooth- or S-form phenotype; the O-antigens are characteristic for a given LPS and the parental bacterial strain
BCSDB Structure5534
Proposed functionsSince all recently investigated LPSs from Acinetobacter strains have been shown to be of the smooth phenotype, a serotyping scheme for identification of members of this genus may also be possible
Antigenic Nature used to produce antibodiesHeat killed bacteria
Carrier NameNil
Conjugation MethodNil
AntibodiesMab S48-3-17
Antibody type and classIgG1
Assay SystemDot blot and Enzyme Immunoassay
Cross-reactivityThis antibody cross-reacted with 15 strains A. baumannii
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis antibody can be used for strain identification in A. baumanni species
Curator IDAA + AS
Date of Curation05-08-2010
ReferencesPMC103717


Entry No. - 3   [TOP]
PolysacDB ID1503
Carbohydrate NameO-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeAcinetobacter baumannii strain 24   (NCBI Taxonomy)   (Drugpedia)
Basic StructureAcinetobacter LPSs consist of a polysaccharide covalently linked to a lipid component, termed lipid A, which anchors the LPS in the outer membrane. This polysaccharide is divided into the core oligosaccharide (linked to lipid A) and the O-polysaccharide or O-antigen. This type of LPS is referred to as the smooth- or S-form phenotype; the O-antigens are characteristic for a given LPS and the parental bacterial strain
BCSDB Structure5534
Proposed functionsSince all recently investigated LPSs from Acinetobacter strains have been shown to be of the smooth phenotype, a serotyping scheme for identification of members of this genus may also be possible
Antigenic Nature used to produce antibodiesHeat killed bacteria
Carrier NameNil
Conjugation MethodNil
AntibodiesPolysera
Antibody type and classN/A
Assay SystemEnzyme immunoassay
Cross-reactivityThe antisera cross-reacted with 13 strains A. baumannii
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab can be used to detect the presence of bacterial antigens in lungs from infected animals
Curator IDAA + AS
Date of Curation06-08-2010
ReferencesPMC103717


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