PolysacDB ID | 2290 |
Carbohydrate Name | O-polysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Shigeila sonnei serotype D (NCBI Taxonomy) (Drugpedia) |
Basic Structure | Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
|
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Heat killed cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab Sh5S |
Antibody type and class | IgM |
Assay System | ELISA and immunoblotting |
Cross-reactivity | O-polysaccharide-specific MAb Sh5S recognized LPS molecules from different phase I strains of S. sonnei as well as from strains M51 and C27 of Plesiomonas shigelloides, reported to belong to the same O serotype as S. sonnei. Furthermore, O-antigen determinants expressed by the S. typhi hybrid strain TY37 were recognized. |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This Mab has been used to analyze clinical isolates and a hybrid Salmonella typhi strain for their expression of the corresponding LPS moieties. In addition to their use in the screening of candidate vaccine strains, the new MAb provide a powerful tool for epidemiological and phylogenetic studies of natural enterobacterial populations |
Curator ID | AA + AS |
Date of Curation | 20-03-2011
|
References | PMC257229 |