PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 1

Entry No. - 1   [TOP]
PolysacDB ID2060
Carbohydrate NameO-polysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobePseudomonas syringae IMV 120a pv. Phaseolicola   (NCBI Taxonomy)   (Drugpedia)
Basic StructureO-polysaccharides of the LPS consists of tetra- and tri-α-D-rhamnose repeats in the backbone [-3)D-Rha(α1->3)D-Rha(α1-->2)D-Rha(α1->2)D-Rha(α1] and [3)D-Rha(α1-->3)D-Rha(α1-->2)D-Rha(α1-]
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab Ps1d
Antibody type and classIgM
Assay SystemAgglutination, Ouchterlony immunodiffusion, ELISA, SDS-PAGE, and Western immunoblotting
Cross-reactivity"This Mab reacted with P. syringae strains with pathovars atrofaciens, phaseolicola and 17 (80%) P. syringae strains for which the pathovar(s) has not been identified"
Proposed epitopesThe epitope was confined to lateral immunodominant substituents of (α1-->3)D-Rha, (β1-->4)D-GlcNAc, and (α1-->4)D-Fuc, respectively, which are all bound to the backbone-located D-rhamnose
IEDB EpitopeN/A
Proposed UtilityThis Mab strongly agglutinated homologous bacterial cells and precipitated soluble antigens
Curator IDAA + AS
Date of Curation02-01-2011
ReferencesPMC178531


Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020