|
|
|
|
Home | Search | Advance Search | Browse | Online Submission | Documentation & FAQ | Team | Contact us |
|
| PolysacDB ID | 2277 |
| Carbohydrate Name | O-polysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri serotype 4a (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1--> |
| BCSDB Structure | 4951 |
| Proposed functions | LPS plays an important virulence role based on its antiphagocytic and endotoxic properties |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab MASF IV-1 |
| Antibody type and class | IgG3 |
| Assay System | ELISA |
| Cross-reactivity | MASF IV-1 antibodies recognized all but one of the S. flexneri 4a strains (O antigens IV:3,4) and all serotype 4 strains (O antigen IV only; IV:-), but neither of the two 4b (O antigens IV:6) strains available |
| Proposed epitopes | α-D-Glcp-1-->6-β-D-GlcpN residues seemed to be the part of the epitope |
| IEDB Epitope | N/A |
| Proposed Utility | This Mab may prove to be valuable reagents in the studies of protective immunity against Shigella bacteria |
| Curator ID | AA + AS |
| Date of Curation | 16-03-2011 |
| References | 2437036 |
| PolysacDB ID | 2329 |
| Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
| Carbohydrate Class | Lipopolysaccharide |
| Microbe | Shigella flexneri (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1--> |
| BCSDB Structure | 3707 |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Heat killed cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab MASF IV-1 |
| Antibody type and class | IgG |
| Assay System | ELISA, slide agglutination |
| Cross-reactivity | This Mab cross-reacted with serotype 4a, 6, X and Y |
| Proposed epitopes | This Mab was directed against antigen E1037 |
| IEDB Epitope | 76815 |
| Proposed Utility | This Mab can be useful for antigen detection in stool samples. Furthermore, use of the panel of monoclonal antibodies made it possible to identify two strains (Y394 and Y400) as putative members of a new provisional serotype of S. flexneri |
| Curator ID | AA + AS |
| Date of Curation | 03-04-2011 |
| References | PMC267520 |
|
Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020 |