PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 1

Entry No. - 1   [TOP]
PolysacDB ID2035
Carbohydrate NameCore oligosaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobePseudomonas aeruginosa   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core region of LPS is divided into the outer core and inner core. The inner core region consists of 2-keto-3-deoxyoctonic acid (KDO), heptose, phosphate, and probably ethanolamine. The outer core region commonly contains D-glucose, L-rhamnose, D-galactosamine, and L-alanine. The O-antigens consist of a trisaccharide repeating unit which consists of two uronic acid derivatives and one N-acetyl fucosamine residue
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesFormalin killed whole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab KN-2B11
Antibody type and classIgM
Assay SystemCompetitive enzyme immunoassay, ELISA, immunoblotting
Cross-reactivity"This MAb specifically bound to clinical isolates belonging to Homma serotypes A, F, G and K at a frequency of 70 to 86% and to serotypes H and M isolates at about 50%. They did not bind to any isolates of serotype B, E, and I tested."
Proposed epitopesα-L-rhamnose residues in the outer core of LPS might be the part of the epitope.
IEDB EpitopeN/A
Proposed UtilityThis Mab agglutinated P. aeruginosa cells
Curator IDAA + AS
Date of Curation23-12-2010
ReferencesPMC313341


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