| PolysacDB ID | 2638 |
| Carbohydrate Name | De-O-acetylated Glucurunoxylomannan (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Cryptococcus neoformans serotype A (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | A linear α(1-->3)- linked mannan backbone singly substituted with non-reducing β(1-->2) xylose and β(1-->2) glucuronic acid side branches ; O-acetyl groups are present at C-6 of the mannosyl residues
|
| BCSDB Structure | 9353 |
| Proposed functions | Antiphagocytic ; an important virulence factor |
| Antigenic Nature used to produce antibodies | Glycoconjugates |
| Carrier Name | Sheep erythrocytes |
| Conjugation Method | The capsular polysaccharide was adsorbed with 0.1 ml of packed sheep erythrocytes for 2 hours at room temperature |
| Antibodies | Mab F12D2 |
| Antibody type and class | IgG3 |
| Assay System | ELISA |
| Cross-reactivity | This antibody cross-reacted with Serotypes A, B, C and D |
| Proposed epitopes | Studies on this antibody showed that there are 2 types of epitopes on GXM-O-acetyl dependent and O-acetyl independent. MAb F12D2 also fails to react with xylose deficient GXM shopwing that xylose residues form an important part of the epitope |
| IEDB Epitope | N/A |
| Proposed Utility | This antibody was used in the elucidation of the probable epitopes present in glucurunoxylomannan. This antibody showed that the induction of a MAb that is reactive with all serotypes does not inherently require the presence of an O-acetyl group on the immunizing GXM |
| Curator ID | AA + AS |
| Date of Curation | 28-07-2011
|
| References | 12965925 |