PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 1

Entry No. - 1   [TOP]
PolysacDB ID1825
Carbohydrate NameCapsular polysaccharide   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeNeisseria meningitidis Serogroup C   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt consists of a homopolymer of α(2-->9)-linked sialic acid residues.
BCSDB Structure130704
Proposed functionsIt is an important virulence factor
Antigenic Nature used to produce antibodiesGlycoconjugates as well as purified capsular polysaccharide
Carrier NameTetanus toxoid
Conjugation MethodThe oligosaccharide was activated using NaIO4 at 4°C for 60 min and combined with monomeric tetanus toxoid in sodium phosphate buffer containing NaBH3CN and heated at 37°C for 5 days
AntibodiesMab CP882.2
Antibody type and classIgG1
Assay SystemELISA
Cross-reactivityThis Mab did not cross-react with the non-O-acetylated variant of capsular polysaccharide and also did not react with E. coli K92 capsular polysaccharide
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab can be used for studying the capsular polysaccharide immunogenicity
Curator IDAA + AS
Date of Curation09-11-2010
ReferencesPMC97127


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