PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 4

Entry No. - 1   [TOP]
PolysacDB ID1265
Carbohydrate NameUnknown carbohydrate   (Drugpedia)
Carbohydrate ClassGlycoprotein
MicrobeCandida albicans   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab C6
Antibody type and classIgM
Assay SystemELISA, immunoelecton microscopy and agglutination tests
Cross-reactivityThis antibody cross-reacted with 30 strains of Candida albicans strains, 2 strains of Candida tropicalis but did not cross-react with Candida paratropicalis, Candida parapsilosis, T. glabrata and S. cerevisae.
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab had a agglutinating activity against Candida albicans
Curator IDAA + AS
Date of Curation02-05-2010
References3280163


Entry No. - 2   [TOP]
PolysacDB ID1334
Carbohydrate NameUnknown carbohydrate   (Drugpedia)
Carbohydrate ClassGlycoprotein
MicrobeCandida albicans 3153A   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesCell wall extract of wild-type Candida albicans 3153A
Carrier NameNil
Conjugation MethodNil
AntibodiesMab C6
Antibody type and classN/A
Assay SystemELISA, Immunofluorescence and immunoblotting
Cross-reactivityThis antibody cross-reacted with strain 62, B, 4C, 24, 37, 53, and 22 but did not react with 4A, 16, 28 and 47 strains
Proposed epitopesThe epitope is supposed to contain high concentrations of glucose and mannose and is present in the acid-labile oligomannosaccharides.
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation18-06-2010
ReferencesPMC281022


Entry No. - 3   [TOP]
PolysacDB ID1341
Carbohydrate NamePhosphopeptidomannan   (Drugpedia)
Carbohydrate ClassGlycoprotein
MicrobeCandida albicans   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab C6
Antibody type and classN/A
Assay SystemELISA, immunoblotting
Cross-reactivityN/A
Proposed epitopesThe epitope consiosts of mannosyl residues from mannobiose to mannoheptaose are bound through β(1-->2) linkage
IEDB EpitopeN/A
Proposed UtilityThis Mab helped in epitope elucidation of the mannans present on Candidial surfaces
Curator IDAA + AS
Date of Curation22-06-2010
ReferencesPMC257398


Entry No. - 4   [TOP]
PolysacDB ID2553
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeVibrio cholerae 01 Inaba 569B strain   (NCBI Taxonomy)   (Drugpedia)
Basic StructureIt is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB StructureN/A
Proposed functionsV. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodiesPurified LPS
Carrier NameNil
Conjugation MethodNil
AntibodiesMab C6
Antibody type and classIgG3
Assay SystemEnzyme-linked immunosorbent assay inhibition experiments
Cross-reactivityThis Mab was specific to Inaba 35 and 569B LPS only
Proposed epitopesThis Mab was specific to C antigen
IEDB EpitopeN/A
Proposed UtilityThis Mab had a good agglutinating capacity when tested against V. cholerae 0:1 strains
Curator IDAA + AS
Date of Curation25-06-2011
ReferencesPMC270838


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