PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 2

Entry No. - 1   [TOP]
PolysacDB ID1054
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeEscherichia coli strain J5 (chemotype Rc)   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe core oligosaccharide part of the LPS consists of the following residues : α-D-Glcp(1-->2)α-D-Glcp(1-->2)α-D-Glcp [branched to α-D-GlcpNAc(1-->3)] (1-->3)α-L-D-man-Hepp [branched to α-D-GlcpNAc(1-->7)α-L-D-man-Hepp(1-->7)] and [branched to a phosphate group] (1-->3)α-L-D-man-Hepp [branched to α-Kdop(2-->4)α-Kdop(2-->4)α-Kdop(2-6)Subst] and [branched to a phosphate group](1-->5)
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 8
Antibody type and classIgG3
Assay SystemELISA, Limulus assay, invivo protection assay
Cross-reactivityThis antibody ross-reacted with Salmonella minnesota strain R5
Proposed epitopesTerminal glucose residues
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation16-01-2010
References2455054


Entry No. - 2   [TOP]
PolysacDB ID2349
Carbohydrate NameK antigens [Capsular polysaccharide]   (Drugpedia)
Carbohydrate ClassCapsular polysaccharide
MicrobeSinorhizobium meliloti strain NRG185   (NCBI Taxonomy)   (Drugpedia)
Basic StructureThe K antigens of Sinorhizobium spp. are major strain-specific antigens, which commonly consist of small repeating units of a hexose and 1-carboxy-2-keto-3-deoxy sugars, such as sialic acid or 3-deoxy-D-manno-2-octulosonic acid (Kdo), although they vary in glycosyl residue composition, substitution patterns, linkage points, anomeric configuration, and size range. The K-antigens of rhizobia do not possess a lipid anchor
BCSDB StructureN/A
Proposed functionsK antigens may be involved in an early recognition step in S. meliloti-alfalfa interactions and that K antigen production is affected by plant-derived signals
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 8
Antibody type and classIgGM
Assay SystemELISA and immunoblotting
Cross-reactivityThis Mab was specific to S. melliloti. But MAb 8 recognized the K antigens from both the homologous strain, S. meliloti NRG185, and S. meliloti NRG286
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThe use of MAbs to study specific cell surface components from the bacteroids of Rhizobium leguminosarum have yielded interesting information about morphogenesis in the pea symbiont, including the temporal expression of specific epitopes during nodule development
Curator IDAA + AS
Date of Curation11-04-2011
References10543844


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