PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 1

Entry No. - 1   [TOP]
PolysacDB ID1069
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeEscherichia coli C600   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole formalinized E. coli C600 cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 786
Antibody type and classIgG3
Assay SystemELISA and immunoblotting
Cross-reactivityThis antibody cross-reacted with LPS core types K-12, R4, and R2 but did not cross-react with Rl LPS and R3 LPS,
Proposed epitopesThe epitope was proposed to consist of trisaccharide sequence Hexp(1->2)-α-D -Glcp(1->3)α-D-Glcp->(Hepp) at the inner region of the outer core, in which the heptose-distal glucose is immunodominant
IEDB EpitopeN/A
Proposed UtilityThis Mab may be used for the detection of LPS core structures but also for analysis of the molecular forms resolved on polyacrylamide gels (banding patterns)
Curator IDAA + AS
Date of Curation21-01-2010
ReferencesPMC261975


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