PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

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Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613
Total Entries - 2

Entry No. - 1   [TOP]
PolysacDB ID1072
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeEscherichia coli Olll:B4   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 5B10
Antibody type and classIgG2a
Assay SystemELISA, immunoblotting, radioimmunoassay, invivo protection assay
Cross-reactivityThis antibody did not bind to LPS from E. coli 55, an Rc mutant of E. coli 011 1:B4 that lacks both the O-antigen and outer core sugars. 5B10 did not crossreact with LPS from several other E. coli strains
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityThis Mab was able to protect mice from a lethal challenge of E. coli 0111:B4 organisms
Curator IDAA + AS
Date of Curation22-01-2010
References3298431


Entry No. - 2   [TOP]
PolysacDB ID1393
Carbohydrate NameLipopolysaccharide   (Drugpedia)
Carbohydrate ClassLipopolysaccharide
MicrobeEscherichia coli J5   (NCBI Taxonomy)   (Drugpedia)
Basic StructureN/A
BCSDB StructureN/A
Proposed functionsN/A
Antigenic Nature used to produce antibodiesWhole cells
Carrier NameNil
Conjugation MethodNil
AntibodiesMab 5B10
Antibody type and classIgG1
Assay SystemELISA, immunoblotting and RIA
Cross-reactivityThis antibody was specific to E. coli J5 and did not cross-react with any other bacteria
Proposed epitopesN/A
IEDB EpitopeN/A
Proposed UtilityN/A
Curator IDAA + AS
Date of Curation08-07-2010
ReferencesPMC260436


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