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PolysacDB ID | 1038 |
Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Rhizobium leguminosarum (NCBI Taxonomy) (Drugpedia) |
Basic Structure | LPS is composed of three parts: (1) lipid A, (2) core polysaccharide, and (3) O antigen. The lipid A region contains two glucosamine sugar derivatives, each with three fatty acids and phosphate or pyrophosphate attached. The core polysaccharide has KDO and attached to lipid A. The side chain O is small polysaccharide chain extending outward from the core, it has several peculiar sugars and varies in composition between bacterial strains. |
BCSDB Structure | 1686 |
Proposed functions | LPS is most unusual constituents of outer membrane. It plays important role in host defence avoidance. LPS contributes to the negative charge of the bacterial surface. Lipid A is often toxic and act as endotoxin |
Antigenic Nature used to produce antibodies | Mixture of suspension of cell envelop. |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 3 |
Antibody type and class | N\A |
Assay System | Western blotting, spot imunoassays, agglutination tests |
Cross-reactivity | This antibody was cross-reactive with different strains of R.leguminosarum like R.biovars viciae, trifolli and phaseoli. |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | N\A |
Curator ID | AA + AS |
Date of Curation | 10-01-2010 |
References | PMC209711 |
PolysacDB ID | 1052 |
Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Escherichia coli strain J5 (chemotype Rc) (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The core oligosaccharide part of the LPS consists of the following residues : α-D-Glcp(1-->2)α-D-Glcp(1-->2)α-D-Glcp [branched to α-D-GlcpNAc(1-->3)] (1-->3)α-L-D-man-Hepp [branched to α-D-GlcpNAc(1-->7)α-L-D-man-Hepp(1-->7)] and [branched to a phosphate group] (1-->3)α-L-D-man-Hepp [branched to α-Kdop(2-->4)α-Kdop(2-->4)α-Kdop(2-6)Subst] and [branched to a phosphate group](1-->5) |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 3 |
Antibody type and class | IgG3 |
Assay System | ELISA, Limulus assay, invivo protection assay |
Cross-reactivity | This antibody ross-reacted with Salmonella minnesota strain R5 |
Proposed epitopes | Terminal glucose residues |
IEDB Epitope | N/A |
Proposed Utility | N/A |
Curator ID | AA + AS |
Date of Curation | 16-01-2010 |
References | 2455054 |
PolysacDB ID | 1484 |
Carbohydrate Name | Unknown carbohydrate (Drugpedia) |
Carbohydrate Class | Glycoprotein |
Microbe | Histoplasma capsulatum (NCBI Taxonomy) (Drugpedia) |
Basic Structure | N/A |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Disrupted yeast cell homogenate of Histoplasma capsulatum |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 3 |
Antibody type and class | IgG1 |
Assay System | ELISA, immunoblotting and Solid-phase radioimmunoassay |
Cross-reactivity | MAb 3 cross-reacted with B. dermatitidis, Paracoccidioides brasiliensis, Sporothrir schenckii, and Candida albicans |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | MAbs may prove useful in the development of more specific immunodiagnostic tests for histoplasmosis |
Curator ID | AA + AS |
Date of Curation | 31-07-2010 |
References | PMC313195 |
PolysacDB ID | 2594 |
Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Rhizobium leguminosarum biovar viciae strain 248 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | N/A |
BCSDB Structure | N/A |
Proposed functions | Structural modifications of Rhizobium LPS may play an important role in the adaptation of endosymbiotic rhizobia to the surrounding microenvironment |
Antigenic Nature used to produce antibodies | Peptidoglycan-outer membrane complex |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 3 |
Antibody type and class | N/A |
Assay System | ELISA, immunoblotting, Spot immunoassays, Agglutination tests |
Cross-reactivity | This Mab was specific to the immunizing strain |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | This Mab can be a useful tool for studying the differentiation process from free-living bacteria to bacteroids |
Curator ID | AA + AS |
Date of Curation | 13-07-2011 |
References | PMC209711 |
Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020 |