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| PolysacDB ID | 1104 |
| Carbohydrate Name | High molecular weight polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Actinobacillus pleuropneumoniae serotype 4 (field isolate F6) (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | N/A |
| BCSDB Structure | N/A |
| Proposed functions | N/A |
| Antigenic Nature used to produce antibodies | Formalin-fixed A. pleuropneumoniae serotype 4 cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab 1E10 |
| Antibody type and class | IgM |
| Assay System | Whole cell ELISA and slide agglutination tests |
| Cross-reactivity | This Mab failed to recognize surface epitopes located on other bacterial, such as H. parasuis, P. haemolytica, P. multocida capsular types A and D, Haemophilus taxon minor group, and A. suis, K. pneumoniae and B. bronchiseptica. This Mab exhibited 75.8 and 27.3% reactivities with 29 serotype 4 and 12 serotype7 isolates, respectively, and partial binding to serotype 1 and nontypeable isolates |
| Proposed epitopes | N/A |
| IEDB Epitope | N/A |
| Proposed Utility | This MAb could be useful for the serological diagnostic of animals infected with serotypes 4 and 7 by inhibition ELISA or competition ELISA. Moreover, they could be useful as complementary reagents to polyclonal sera for serotyping of field isolates belonging to A. pleuropneumoniae serotype 4 or 7 |
| Curator ID | AA + AS |
| Date of Curation | 30-01-2010 |
| References | PMC170200 |
| PolysacDB ID | 1499 |
| Carbohydrate Name | Capsular polysaccharide (Drugpedia) |
| Carbohydrate Class | Capsular polysaccharide |
| Microbe | Cryptococcus neoformans Serotype A [strain 24064] (NCBI Taxonomy) (Drugpedia) |
| Basic Structure | The major component of C. neoformans capsular polysaccharide is glucurunoxylomannan [GXM] consisting of an unbranched mannose backbone substituted with xylose, glucuronic acid and O-acetyl residues |
| BCSDB Structure | N/A |
| Proposed functions | C. neoformans has a large polysaccharide capsule that is a determinant of virulence. The capsular polysaccharide causes immunologic paralysis and inhibits phagocytosis by macrophages. Soluble C. neoformans capsular polysaccharide enhances human immunodeficiency virus [HIV] infection in cultured cells, suggesting a new pathogenic role in AIDS |
| Antigenic Nature used to produce antibodies | Whole cells |
| Carrier Name | Nil |
| Conjugation Method | Nil |
| Antibodies | Mab 1E10 |
| Antibody type and class | IgM |
| Assay System | ELISA |
| Cross-reactivity | This antibody reacted with 2 serotype A strains 24064 and 371 |
| Proposed epitopes | This Mab did not bind to de-O-acetylated GXM from both serotype A strains. This shows that acetylated residues form an important part of the epitope |
| IEDB Epitope | N/A |
| Proposed Utility | N/A |
| Curator ID | AA + AS |
| Date of Curation | 05-08-2010 |
| References | 1583327 |
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Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020 |