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PolysacDB ID | 1048 |
Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Escherichia coli strain J5 (chemotype Rc) (NCBI Taxonomy) (Drugpedia) |
Basic Structure | The core oligosaccharide part of the LPS consists of the following residues : α-D-Glcp(1-->2)α-D-Glcp(1-->2)α-D-Glcp [branched to α-D-GlcpNAc(1-->3)] (1-->3)α-L-D-man-Hepp [branched to α-D-GlcpNAc(1-->7)α-L-D-man-Hepp(1-->7)] and [branched to a phosphate group] (1-->3)α-L-D-man-Hepp [branched to α-Kdop(2-->4)α-Kdop(2-->4)α-Kdop(2-6)Subst] and [branched to a phosphate group](1-->5) |
BCSDB Structure | N/A |
Proposed functions | N/A |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 10 |
Antibody type and class | IgG3 |
Assay System | ELISA, Limulus assay, invivo protection assay |
Cross-reactivity | This antibody was specific to E. coli J5 |
Proposed epitopes | Terminal glucose residues |
IEDB Epitope | N/A |
Proposed Utility | N/A |
Curator ID | AA + AS |
Date of Curation | 15-01-2010 |
References | 2455054 |
PolysacDB ID | 2351 |
Carbohydrate Name | Lipopolysaccharide (Drugpedia) |
Carbohydrate Class | Lipopolysaccharide |
Microbe | Sinorhizobium meliloti strain NRG34 (NCBI Taxonomy) (Drugpedia) |
Basic Structure | There are two forms of LPS: rough LPS (R-LPS), which consists of a lipid A membrane anchor and conserved core oligosaccharides, and smooth LPS (S-LPS), which includes the O-antigen (or O-polysaccharide) |
BCSDB Structure | N/A |
Proposed functions | LPS is an important surface antigen |
Antigenic Nature used to produce antibodies | Whole cells |
Carrier Name | Nil |
Conjugation Method | Nil |
Antibodies | Mab 10 |
Antibody type and class | IgG3 |
Assay System | ELISA and immunoblotting |
Cross-reactivity | This Mab was highly cross-reactive recognizing most number of Shinorhizobium species. Strain-cross-reactive MAb 10 appeared to recognize several forms of R-LPS, as shown by the disperse signal in the R-LPS banding region, as well as the S-LPS from the same strains |
Proposed epitopes | N/A |
IEDB Epitope | N/A |
Proposed Utility | The use of MAbs to study specific cell surface components from the bacteroids of Rhizobium leguminosarum have yielded interesting information about morphogenesis in the pea symbiont, including the temporal expression of specific epitopes during nodule development |
Curator ID | AA + AS |
Date of Curation | 11-04-2011 |
References | 10543844 |
Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020 |