PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

PolysacDB: A comprehensive database of microbial polysaccharide antigens and their antibodies

Citation: Aithal A, Sharma A, Joshi S, Raghava GPS, Varshney GC (2012) PolysacDB: A Database of Microbial Polysaccharide Antigens and Their Antibodies. PLoS ONE 7(4): e34613. doi:10.1371/journal.pone.0034613

Total Entries - 41
Entry No. - 1 [TOP]

PolysacDB ID	1114
Carbohydrate Name	O-polysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Actinobacillus pleuropneumoniae serotype 5b (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure	108568
Proposed functions	LPS is an important virulence factor. It molecule plays an important role in adherence of the bacterium to porcine respiratory tract cells and mucus
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	N/A
Assay System	Elisa inhibition assay
Cross-reactivity	This antibody reacted only with the reference strains of Actinobacillus serotypes 5
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This Mab can be used for identification of serotype5 cells
Curator ID	AA + AS
Date of Curation	02-02-2010
References	PMC1189361

Entry No. - 2 [TOP]

PolysacDB ID	1613
Carbohydrate Name	PTP1 antigen (Drugpedia)
Carbohydrate Class	Glycoprotein
Microbe	Encephalitozoon intestinalis (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Infected patient
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	N/A
Assay System	Indirect immunofluorescence, Western blotting, two-dimensional gel electrophoresis, and chemical deglycosylation
Cross-reactivity	This antisera cross-reacted with E. hellem and E. cuniculi
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antisera was found to decrease the infectivity of E. intestinalis in vitro
Curator ID	AA + AS
Date of Curation	23-09-2010
References	PMC1307029

Entry No. - 3 [TOP]

PolysacDB ID	1903
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Pasteurella multocida serotype 1 (NCBI Taxonomy) (Drugpedia)
Basic Structure	LPS molecule is composed of three regions consisting of lipid A, core oligosaccharides, and O-antigen polysaccharides. The O-antigen part consists of the following residues : -3)-β-D-Galp-(1-->3)-β-D-GalpNAc-(1-->4)-β-D-Galp-(1-. The core oligosaccharide part consists of the following residues : β-D-Galp-(1-->7)-D-gro-α-D-manHepp-(1-->6)-D-gro-α-D-manHepp-(1-->6)-β-D-Glcp-(1-->4)-L-gro [branched to L-gro-α-D-manHepp-(1-->2)-L-gro-α-D-manHepp-(1-->3)] and [branched to α-D-Glcp-(1-->6)] -α-D-manHepp-(1--/lipid A
BCSDB Structure	N/A
Proposed functions	LPS is well known for its biological activities, including modification of metabolic activity and phagocytic function, alteration of migration of neutrophils and macrophages, and mitogenesis of lymphocytes
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	N/A
Assay System	ELISA
Cross-reactivity	This antisera was specific to serotype 1 cells
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antisera may be useful in defining antigenic determinants of LPS.
Curator ID	AA + AS
Date of Curation	25-11-2010
References	PMC271284

Entry No. - 4 [TOP]

PolysacDB ID	1904
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Pasteurella multocida serotype 15 (NCBI Taxonomy) (Drugpedia)
Basic Structure	LPS molecule is composed of three regions consisting of lipid A, core oligosaccharides, and O-antigen polysaccharides
BCSDB Structure	N/A
Proposed functions	LPS is well known for its biological activities, including modification of metabolic activity and phagocytic function, alteration of migration of neutrophils and macrophages, and mitogenesis of lymphocytes
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	N/A
Assay System	ELISA
Cross-reactivity	This antisera was specific to serotype 15 cells
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antisera may be useful in defining antigenic determinants of LPS.
Curator ID	AA + AS
Date of Curation	25-11-2010
References	PMC271284

Entry No. - 5 [TOP]

PolysacDB ID	1905
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Pasteurella multocida serotype 4 (NCBI Taxonomy) (Drugpedia)
Basic Structure	LPS molecule is composed of three regions consisting of lipid A, core oligosaccharides, and O-antigen polysaccharides
BCSDB Structure	N/A
Proposed functions	LPS is well known for its biological activities, including modification of metabolic activity and phagocytic function, alteration of migration of neutrophils and macrophages, and mitogenesis of lymphocytes
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	N/A
Assay System	ELISA
Cross-reactivity	This antisera cross-reacted with serotype 12 cells
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antisera may be useful in defining antigenic determinants of LPS.
Curator ID	AA + AS
Date of Curation	25-11-2010
References	PMC271284

Entry No. - 6 [TOP]

PolysacDB ID	1906
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Pasteurella multocida serotype 12 (NCBI Taxonomy) (Drugpedia)
Basic Structure	LPS molecule is composed of three regions consisting of lipid A, core oligosaccharides, and O-antigen polysaccharides
BCSDB Structure	N/A
Proposed functions	LPS is well known for its biological activities, including modification of metabolic activity and phagocytic function, alteration of migration of neutrophils and macrophages, and mitogenesis of lymphocytes
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	N/A
Assay System	ELISA
Cross-reactivity	This antisera cross-reacted with serotype 4 cells
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antisera may be useful in defining antigenic determinants of LPS.
Curator ID	AA + AS
Date of Curation	25-11-2010
References	PMC271284

Entry No. - 7 [TOP]

PolysacDB ID	1999
Carbohydrate Name	Disaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Streptococcus pneumoniae serotype 6B (NCBI Taxonomy) (Drugpedia)
Basic Structure	The structure of the disaccharide is α-L-Rhap-(1-->4)-D-Rib-ol-[5-->P-->(CH2)3NH2]
BCSDB Structure	5205
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Keyhole limpet hemocyanin
Conjugation Method	The saccharides were coupled via the 3-aminopropyl spacer to carrier protein KLH in a stepwise reaction: the amino-terminal groups of the OS fragment and KLH were activated by S acetylation and Br acetylation, respectively, and coupled via a thioether linkage
Antibodies	Antisera
Antibody type and class	N/A
Assay System	ELISA, phagocytosis assays and protection experiments
Cross-reactivity	This antisera was specific to capsular polysaccharide of strain 6B and did not cross-react with 6A strains
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antisera could passively protect mice against a 6B challenge
Curator ID	AA + AS
Date of Curation	14-12-2010
References	PMC97953

Entry No. - 8 [TOP]

PolysacDB ID	2000
Carbohydrate Name	Trisaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Streptococcus pneumoniae serotype 6B (NCBI Taxonomy) (Drugpedia)
Basic Structure	The structure of the trisaccharide is -D-Rib-ol-(5-->P-->2)-α-D-Galp-(1-->3)-α-D-Glcp-[1-->O(CH2)3NH2]
BCSDB Structure	5205
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Keyhole limpet hemocyanin
Conjugation Method	The saccharides were coupled via the 3-aminopropyl spacer to carrier protein KLH in a stepwise reaction: the amino-terminal groups of the OS fragment and KLH were activated by S acetylation and Br acetylation, respectively, and coupled via a thioether linkage
Antibodies	Antisera
Antibody type and class	N/A
Assay System	ELISA, phagocytosis assays and protection experiments
Cross-reactivity	This antisera was specific to capsular polysaccharide of strain 6B and did not cross-react with 6A strains
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antisera could passively protect mice against a 6B challenge
Curator ID	AA + AS
Date of Curation	14-12-2010
References	PMC97953

Entry No. - 9 [TOP]

PolysacDB ID	2001
Carbohydrate Name	Tetrasaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Streptococcus pneumoniae serotype 6B (NCBI Taxonomy) (Drugpedia)
Basic Structure	The structure of the trisaccharide is α-L-Rhap-(1-->4)-D-Rib-ol-(5-->P-->2)-α-D-Galp-(1-->3)-α-D-Glcp-[1-->O(CH2)3NH2]
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Keyhole limpet hemocyanin
Conjugation Method	The saccharides were coupled via the 3-aminopropyl spacer to carrier protein KLH in a stepwise reaction: the amino-terminal groups of the OS fragment and KLH were activated by S acetylation and Br acetylation, respectively, and coupled via a thioether linkage
Antibodies	Antisera
Antibody type and class	N/A
Assay System	ELISA, phagocytosis assays and protection experiments
Cross-reactivity	This antisera cross-reacted with capsular polysaccharide of 6A strains
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antisera could passively protect mice against a 6B and 6A challenge
Curator ID	AA + AS
Date of Curation	14-12-2010
References	PMC97953

Entry No. - 10 [TOP]

PolysacDB ID	2071
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Pseudomonas aeruginosa strain Fisher IT-3 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core oligosaccharide of P. aeruginosa can be divided into outer and inner core regions. The inner core consists of 2-keto-3-deoxyoctonate (Kdo), heptose, and large amounts of phosphate and is believed to be homogeneous among serotypes. The outer core of P. aeruginosa contains D-glucose (D-Glc), L-rhamnose (L-Rha), D-galactosamine (D-GalN), and L-alanine (L-Ala]
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Alkali treated LPS
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	N/A
Assay System	ELISA, opsonophagocytic assays, animal protection studies and immunoblotting
Cross-reactivity	This antisera was highly cross-reactive
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antisera had opsonophagocytic activity against LPS rough strains
Curator ID	AA + AS
Date of Curation	07-01-2011
References	PMC172952

Entry No. - 11 [TOP]

PolysacDB ID	2072
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Pseudomonas aeruginosa strain Fisher IT-4 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core oligosaccharide of P. aeruginosa can be divided into outer and inner core regions. The inner core consists of 2-keto-3-deoxyoctonate (Kdo), heptose, and large amounts of phosphate and is believed to be homogeneous among serotypes. The outer core of P. aeruginosa contains D-glucose (D-Glc), L-rhamnose (L-Rha), D-galactosamine (D-GalN), and L-alanine (L-Ala]
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Alkali treated LPS
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	N/A
Assay System	ELISA, opsonophagocytic assays, animal protection studies and immunoblotting
Cross-reactivity	This antisera was specific to strain IT-4
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antisera had opsonophagocytic activity against LPS rough strains
Curator ID	AA + AS
Date of Curation	07-01-2011
References	PMC172952

Entry No. - 12 [TOP]

PolysacDB ID	2073
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Pseudomonas aeruginosa strain AK1401 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The LPS consists of three regions, namely O-polysaccharide, core oligosaccharide and lipid A. The core oligosaccharide of P. aeruginosa can be divided into outer and inner core regions. The inner core consists of 2-keto-3-deoxyoctonate (Kdo), heptose, and large amounts of phosphate and is believed to be homogeneous among serotypes. The outer core of P. aeruginosa contains D-glucose (D-Glc), L-rhamnose (L-Rha), D-galactosamine (D-GalN), and L-alanine (L-Ala]
BCSDB Structure	4396
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Alkali treated LPS
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	N/A
Assay System	ELISA, opsonophagocytic assays, animal protection studies and immunoblotting
Cross-reactivity	This antisera was highly cross-reactive
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antisera had opsonophagocytic activity
Curator ID	AA + AS
Date of Curation	08-01-2011
References	PMC172952

Entry No. - 13 [TOP]

PolysacDB ID	2270
Carbohydrate Name	Y O-polysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Shigella flexneri (NCBI Taxonomy) (Drugpedia)
Basic Structure	The O-LPS consists of a tetrasaccharide repeating unit α-L-Rhap-(1-->2)-α-L-Rhap(1-->3)- . This tetrasaccharide is polymerized and joined by β-1,2 linkages between N-acetyl-D-glucosamine and L-rhamnosyl to form the O-polysaccharide chain
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Heat-killed S. flexnerf bacteria serotype Y
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	N/A
Assay System	ELISA, FARR assay
Cross-reactivity	The polyclonal rabbit anti-group antigen 3.4 antibodies reacted with the lb, 2a, 3b, 4a. 4b, and Y LPS. They did not bind to1a and 5a. In glycoconjugate ELISA's it bound to all saccharides especially abcd- and decasaccharide- BSA antigens
Proposed epitopes	Antigen 3,4 seemed to be the epitope
IEDB Epitope	76696
Proposed Utility	This Mab would help in the delineation of the antigenic stucture of Y O polysaccharide
Curator ID	AA + AS
Date of Curation	16-03-2011
References	2438343

Entry No. - 14 [TOP]

PolysacDB ID	2323
Carbohydrate Name	O-polysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Shigella flexneri (NCBI Taxonomy) (Drugpedia)
Basic Structure	Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
BCSDB Structure	3707
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Heat killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	N/A
Assay System	ELISA
Cross-reactivity	The highest titers seen were against serotype 3a and 3b LPSs
Proposed epitopes	In S. flexneri serotype 3a and 3b LPSs there is no structural feature to which the type III antigen epitope can be assigned. Instead, the serotype specificity is a consequence of the addition of two group antigen epitopes to the basic structure in serotype 3a (7,8 and 6 epitopes) and of one group antigen epitope to the basic structure in serotype 3b (6 epitope). The α-D-glucose-(1-->3)-α-L-rhamnose was assigned as the determinant for group 7,8 antigen, and the 2-O-acetyl-α-L-rhamnose III structure was assigned as the determinant for group 6 antigen
IEDB Epitope	76815
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	02-04-2011
References	PMC96386

Entry No. - 15 [TOP]

PolysacDB ID	2324
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Shigella flexneri [polyvalent S. flexneri 2a/3a vaccine (CVD 1207/CVD 1211)] (NCBI Taxonomy) (Drugpedia)
Basic Structure	Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
BCSDB Structure	N/A
Proposed functions	LPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature used to produce antibodies	Heat killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	IgG
Assay System	ELISA
Cross-reactivity	This antisera strongly cross-reacted with S. flexneri LPS of serotypes 1a and 1b
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	02-04-2011
References	PMC96386

Entry No. - 16 [TOP]

PolysacDB ID	2325
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Shigella flexneri [polyvalent S. flexneri 2a/3a vaccine (CVD 1207/CVD 1211)] (NCBI Taxonomy) (Drugpedia)
Basic Structure	Chemically, the O-antigen is a polysaccharide chain of variable length, built up by repetitive sequences of tetra- to hexasaccharides. It consists of a basic tetrasaccharide repeating unit consisting of three rhamnoses and one N-acetylglucosamine was common to all S. flexneri strains (except serotype 17): -2)-α-L-Rhap-(1-->2)-α-L-Rhap-(1-->3)-α-L-Rhap-(1-->3)-β-D-GlcpNAc-(1-->
BCSDB Structure	N/A
Proposed functions	LPS plays an important virulence role based on its antiphagocytic and endotoxic properties
Antigenic Nature used to produce antibodies	Heat killed cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	IgA
Assay System	ELISA
Cross-reactivity	This antisera showed an extensive cross-reaction that included S. flexneri serotypes 1a, 1b, 2b, 4b, 5b, and Y
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	Cross-protection was observed among S. flexneri serotypes elicited by the S. flexneri 2a/3a vaccine
Curator ID	AA + AS
Date of Curation	02-04-2011
References	PMC96386

Entry No. - 17 [TOP]

PolysacDB ID	2353
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Sinorhizobium meliloti strain RM41 (NCBI Taxonomy) (Drugpedia)
Basic Structure	There are two forms of LPS: rough LPS (R-LPS), which consists of a lipid A membrane anchor and conserved core oligosaccharides, and smooth LPS (S-LPS), which includes the O-antigen (or O-polysaccharide)
BCSDB Structure	N/A
Proposed functions	LPS is an important surface antigen
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	N/A
Assay System	ELISA and immunoblotting
Cross-reactivity	This antisera was specific to the homologous immunizing strain
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	The antisera generated may help in understanding the prevalence of specific LPS sub-structures among various sub-species
Curator ID	AA + AS
Date of Curation	13-04-2011
References	10543844

Entry No. - 18 [TOP]

PolysacDB ID	2354
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Sinorhizobium fredii USDA205 (NCBI Taxonomy) (Drugpedia)
Basic Structure	There are two forms of LPS: rough LPS (R-LPS), which consists of a lipid A membrane anchor and conserved core oligosaccharides, and smooth LPS (S-LPS), which includes the O-antigen (or O-polysaccharide)
BCSDB Structure	N/A
Proposed functions	LPS is an important surface antigen
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	N/A
Assay System	ELISA and immunoblotting
Cross-reactivity	This antisera was specific to the homologous immunizing strain
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	The antisera generated may help in understanding the prevalence of specific LPS sub-structures among various sub-species
Curator ID	AA + AS
Date of Curation	13-04-2011
References	10543844

Entry No. - 19 [TOP]

PolysacDB ID	2386
Carbohydrate Name	Streptococcal polysaccharide antigen eI (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Streptococcus mutans strain MT703 serotype e (NCBI Taxonomy) (Drugpedia)
Basic Structure	The major fraction (eI) was identified as a polysaccharide composed of 37% glucose, 56% rhamnose, 5% protein, and 0.3% phosphorus, whereas the minor fraction (eII) contained 66% protein in addition to 10% glucose and 17% rhamnose
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	N/A
Assay System	Quantitative precipitin test and immunodiffusion and/or immunoelectrophoresis
Cross-reactivity	This antisera cross-reacted with type E
Proposed epitopes	Methyl-β-D-glucopyranoside residues seemed to be the predominant epitope. A β-linked glucose-glucose dimer is the predominant antigenic determinant of the e specificity
IEDB Epitope	N/A
Proposed Utility	This antisera may help in elucidating the antigenic specificity of E and e antigens on the surface of streptococcus mutans
Curator ID	AA + AS
Date of Curation	25-04-2011
References	PMC420845

Entry No. - 20 [TOP]

PolysacDB ID	2387
Carbohydrate Name	Streptococcal polysaccharide antigen eI (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Streptococcus mutans strain K129 serotype E (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	N/A
Assay System	Quantitative precipitin test and immunodiffusion and/or immunoelectrophoresis
Cross-reactivity	This antisera was specific to E1
Proposed epitopes	Methyl-β-D-glucopyranoside residues seemed to be the predominant epitope
IEDB Epitope	N/A
Proposed Utility	This antisera may help in elucidating the antigenic specificity of E and e antigens on the surface of streptococcus mutans
Curator ID	AA + AS
Date of Curation	25-04-2011
References	PMC420845

Entry No. - 21 [TOP]

PolysacDB ID	2388
Carbohydrate Name	Cell wall carbohydrate (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Streptococcus GAS strain J-17 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Infected patients
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	IgG2
Assay System	ELISA
Cross-reactivity	N/A
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	The measurement of the anti- ACHO antibody by ELISA with enzyme-treated whole cells can be a useful, reliable and simple method for serological diagnosis of group A infection and sequelae
Curator ID	AA + AS
Date of Curation	25-04-2011
References	8810949

Entry No. - 22 [TOP]

PolysacDB ID	2389
Carbohydrate Name	Cell wall carbohydrate (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Streptococcus GAS strain J-18 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	IgG2
Assay System	ELISA
Cross-reactivity	N/A
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	The measurement of the anti- ACHO antibody by ELISA with enzyme-treated whole cells can be a useful, reliable and simple method for serological diagnosis of group A infection and sequelae
Curator ID	AA + AS
Date of Curation	26-04-2011
References	8810949

Entry No. - 23 [TOP]

PolysacDB ID	2390
Carbohydrate Name	Type III polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Streptococcus Group B strain M731 and M732 (NCBI Taxonomy) (Drugpedia)
Basic Structure	This polysaccharide has a mol wt of > 5 × 10⁶ daltons. Its main chemical constituents are sialic acid (24%), galactose (25%), heptose (21%), glucose (13%), glucosamine (10%) and mannose (7%)
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	N/A
Assay System	Capillary precipitin tests, immunodiffusion test
Cross-reactivity	This antisera predominantly reacted with type III polysaccharide
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	26-04-2011
References	55450

Entry No. - 24 [TOP]

PolysacDB ID	2409
Carbohydrate Name	Group B antigen (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Streptococcus Group B strain M732 (NCBI Taxonomy) (Drugpedia)
Basic Structure	This polysaccharide had a molecular size > 5 x 10⁶. Its main chemical constituents were sialic acid (24%), galactose (25%), a sugar tentatively identified as heptose (21%), glucose (13%), glucosamine (10%) and mannose (7%)
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Formalin-killed whole cell vaccines
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	N/A
Assay System	Capillary precipitin tests, immunodiffusion tests, immunoelectrophoresis
Cross-reactivity	This antisera cross-reacted with the TCA antigenas well as the EDTA-non pH titrated antigen and the group B antigen extracted with formamide from strain 090R or D136C
Proposed epitopes	L-rhamnose was the immunodominant sugar for the formamide-extracted B antigen
IEDB Epitope	N/A
Proposed Utility	This antisera has helped determine that two antigens that react identically with each other and with similar specificities of antibody, yet with different immunodominant determinants
Curator ID	AA + AS
Date of Curation	02-05-2011
References	80423

Entry No. - 25 [TOP]

PolysacDB ID	2412
Carbohydrate Name	Phosphorylcholine (PC) (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Streptococcus pneumoniae (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	Antibodies directed against phosphorylcholine (PC), a major epitope on the cell wall polysaccharide, protect against pneumococcal infections in mice; the role of anti-PC antibodies in humans, however, remains controversial
Antigenic Nature used to produce antibodies	ASRNKANDYTTEYSASVKGRFIVS [peptide1]
Carrier Name	Bovine serum albumin
Conjugation Method	BSA (5 mg) was dissolved in a 0.1 M sodium citrate solution (pH 5.5; 500 ml) to provide a BSA:peptide ratio of 1:25. Glutaraldehyde (0.1%) was added, and the solution was incubated for 1 h at room temperature. The reaction mixture was dialyzed against phosphate-buffered saline (PBS) for 5 days at 4°C
Antibodies	Antisera
Antibody type and class	N/A
Assay System	ELISA, Opsonization and protection assays
Cross-reactivity	N/A
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This peptide was able to inhibit the binding of anti-PC monoclonal antibodies to PC-BSA
Curator ID	AA + AS
Date of Curation	03-05-2011
References	10992485

Entry No. - 26 [TOP]

PolysacDB ID	2413
Carbohydrate Name	Type V capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Streptococcus Group B (NCBI Taxonomy) (Drugpedia)
Basic Structure	The native type v polysaccharide contains D-glucose, D-galactose, 2-acetamido-2-deoxy-glucose and sialic acid in a molar ratio of 3:2:1:1. It consists of a repeating backbone of -->4)-α-D-Glcp-(1-->4)-α-D-Galp-(1-->4)-β-D-Glcp-(1--> with branching at 6th and 3rd positions
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	N/A
Assay System	ELISA, Ouchterlony diffusion
Cross-reactivity	Antibodies against the typeV capsuled o not cross-reactw ith other GBS capsular types and appear to recognize an epitope which is not as heavily dependent on the presenceo f the terminal side chain sialic acid residues as that of the type Ia, 11, and I11 GBS polysaccharides
Proposed epitopes	β-D-Gal-(1-->4)-β-D-GlcNAc segmenot of the trisaccharide side chain, which is present in the core but not in the backbone polysaccharide, also contributes to the native epitope. The residues of the linear backbone and/or the glucopyranosyl side chain also form part of the antibody-binding epitope
IEDB Epitope	N/A
Proposed Utility	This antisera helped in the determination of immunodominant epitopes in Type V polysaccharide in group B Streptococcus
Curator ID	AA + AS
Date of Curation	04-05-2011
References	2016287

Entry No. - 27 [TOP]

PolysacDB ID	2421
Carbohydrate Name	Type 1 polysaccharide antigen (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Streptococcus sanguis ST3 Serotype I (NCBI Taxonomy) (Drugpedia)
Basic Structure	The type I antigen was a polysaccharide composed of glucose, rhamnose, and N-acetylglucosamine in a molar ratio of 1.4:2.5:1.0.
BCSDB Structure	N/A
Proposed functions	The serotype I antigen may participate in the attachment of S. sanguis to tooth surfaces
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	N/A
Assay System	Quantitative precipitin inhibition tests, Immunodiffusion and immunoelectrophoresis
Cross-reactivity	N/A
Proposed epitopes	α-glucosidic linkage is the immunodeterminant of the type I antigen
IEDB Epitope	N/A
Proposed Utility	This antisera helped in determining the immunodominant region in the Type 1 polysaccharide
Curator ID	AA + AS
Date of Curation	07-05-2011
References	PMC347986

Entry No. - 28 [TOP]

PolysacDB ID	2422
Carbohydrate Name	Capsular polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Streptococcus Group B (NCBI Taxonomy) (Drugpedia)
Basic Structure	The structure of type III polysaccharide is as follows : -3-β-D-Galp-(1-->4)-β-D-Glcp-(1-->6)-β-D-GlcNAcp-(1--> along with the fact that the carboxyl of the -D-NeuNAcp was linked to the O-3 of the adjacent D-galactose to form the type-specific site
BCSDB Structure	N/A
Proposed functions	The property of invasiveness is related to the anti-phagocytic properties conferred to Group B Streptococcus by its capsular polysaccharide
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Tetanus toxoid
Conjugation Method	Type III polysaccharide was activated with cyanogen bromide at pH 10.5 for 6 min at 4°C. Adipic acid dihydrazide was added in 0.5 M NaHCO3 to a final concentration of 0.25 M, pH 8.5. the reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and passed through a 4B-CL Sepharose column. The CP-containing fractions were pooled, dialyzed against sterile pyrogen-free water, and freeze-dried. A solution containing 10 mg each of type III-AH and TT per ml was brought to pH 5.6 with 0.1 N HCl. 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide was added to a final concentration of 0.05 M. The reaction mixture was dialyzed against 0.2 M NaCl at 3 to 8°C and was passed through a 4B-CL Sepharose column
Antibodies	Antisera
Antibody type and class	IgG
Assay System	Double immunodiffusion and capillary precipitation, opsonization assays
Cross-reactivity	This antisera was quite specific and did not react with the structurally related pneumococcus type 14 polysaccharide
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	Conjugate-induced antibodies facilitated opsonization of group B streptococcus type III organisms. The type III-TT conjugate can be a potential vaccine for prevention of neonatal Group B Streptococcal diseases.
Curator ID	AA + AS
Date of Curation	08-05-2011
References	2407652

Entry No. - 29 [TOP]

PolysacDB ID	2426
Carbohydrate Name	Streptococcal A carbohydrate (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Streptococcus Group A (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	This Carbohydrate is highly immunodominant
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	N/A
Assay System	Radioimmunoassay
Cross-reactivity	N/A
Proposed epitopes	The antigenic determinant was known as α hapten that had a estimated molecular weight of 1100 daltons and a rhamnose: glucosamine molar ratio of 6:1
IEDB Epitope	76687
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	10-05-2011
References	4795261

Entry No. - 30 [TOP]

PolysacDB ID	2435
Carbohydrate Name	Trisaccharide part of Streptococcus pneumoniae type 3 polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Streptococcus pneumoniae Type 3 (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Diphtheria toxin mutant (CRM197)
Conjugation Method	The saccharide fragments described above were conjugated to CRM197 using diethyl squarate as linker. Elongation of the saccharides with diethyl squarate was performed in ethanol/0.1M sodium phosphate (pH 6.9). The reaction products were purified by solid-phase extraction and coupled to protein in 0.1m sodium borate buffer at pH 9.5
Antibodies	Antisera
Antibody type and class	N/A
Assay System	Enzyme-linked immunosorbent assay and in-vitro phagocytosis assay
Cross-reactivity	N/A
Proposed epitopes	β-d-Glcp-(1-->3)-β-d-GlcpA-(1-->4)-β-d-Glcp
IEDB Epitope	N/A
Proposed Utility	This antisera provided full protection against challenge with pneumococcal type 3 bacteria
Curator ID	AA + AS
Date of Curation	13-05-2011
References	PMC308892

Entry No. - 31 [TOP]

PolysacDB ID	2461
Carbohydrate Name	Synthetic disaccharide abequose(1-->3)-α-rhamnose representative of Salmonella O-antigen 8 (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Salmonella spp. (NCBI Taxonomy) (Drugpedia)
Basic Structure	Synthetic disaccharide abequose1-->3α-rhamnose
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Glycoconjugate
Carrier Name	Bovine serum albumin
Conjugation Method	N/A
Antibodies	Antisera
Antibody type and class	N/A
Assay System	Immunofluorescence and co-agglutination (COA)
Cross-reactivity	The antiserum correctly identified all 99 Salmonella serogroup C2 and C3 bacteria with O-antigen 8. No fluorescence was seen with 484 Salmonella bacteria belonging to other serogroups or 567 non-Salmonella enteric bacteria
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antisera was used for diagnosis of Salmonella bacteria by indirect immunofluorescence (IFL) and by co-agglutination (COA) using sensitized protein A-containing staphylococci
Curator ID	AA + AS
Date of Curation	23-05-2011
References	373381

Entry No. - 32 [TOP]

PolysacDB ID	2463
Carbohydrate Name	Polysaccharide antigen (PS 02/725) (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Eubacterium saburreum strain 02/725 (NCBI Taxonomy) (Drugpedia)
Basic Structure	This polysaccharide consists of D-glycero-d-galacto-heptose and 6-deoxyheptose residues
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	IgG
Assay System	Precipitin assay and complement binding assay
Cross-reactivity	This antisera was specific to 02/725 polysaccharide
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	24-05-2011
References

Entry No. - 33 [TOP]

PolysacDB ID	2464
Carbohydrate Name	Surface polysaccharide (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Eubacterium saburreum strain T18 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The purified antigen was a homopolysaccharide composed of D-glycero-D-galacto-heptose (Hep.) residues. The structure of the repeating unit in the polysaccharide was: -[-6)-[α Hep.furanosyl-(1-->4)]-β-Hep.pyranosyl- (1-->6)-[α-Hep.furanosyl-(1-->2), α-Hep.furanosyl-(1-->4)]-β- Hep.pyranosyl-(1-)4-->6)-β-Hep.pyranosyl-(1-->. No heptose residues were acetylated
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	N/A
Assay System	Immunodiffusion tests
Cross-reactivity	N/A
Proposed epitopes	The immunodeterminant of the antigenic polysaccharide was D-glycero-D-galacto-heptofuranosyl residues as branched nonreducing terminals
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	25-05-2011
References	1408355

Entry No. - 34 [TOP]

PolysacDB ID	2465
Carbohydrate Name	Polysaccharide antigen PS L13 (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Eubacterium saburreum L13 (NCBI Taxonomy) (Drugpedia)
Basic Structure	This polysaccharide contained ketohexose and glucose residues
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	IgG
Assay System	Double diffusion experiments, Immunoelectrophoresis
Cross-reactivity	N/A
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	Rabbit antiserum to E. saburreum L13 agglutinated the bacteria
Curator ID	AA + AS
Date of Curation	25-05-2011
References	PMC347922

Entry No. - 35 [TOP]

PolysacDB ID	2469
Carbohydrate Name	Surface polysaccharide antigen (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Eubacterium saburreum strain L32 (NCBI Taxonomy) (Drugpedia)
Basic Structure	The PS L32 polymer is built up of repeating trisaccharide units of galactose, ribose and dideoxyhexose
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	IgM
Assay System	Precipitation tests, immunoelectrophoresis and complement fixation
Cross-reactivity	N/A
Proposed epitopes	The dideoxyhexose is the immunodominant sugar
IEDB Epitope	N/A
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	27-05-2011
References	670929

Entry No. - 36 [TOP]

PolysacDB ID	2470
Carbohydrate Name	Surface polysaccharide antigen (Drugpedia)
Carbohydrate Class	Capsular polysaccharide
Microbe	Eubacterium saburreum strain T18 (NCBI Taxonomy) (Drugpedia)
Basic Structure	This antigen was composed of D-glycero-D-galacto-heptose
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cells
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	IgM
Assay System	Immunoprecipitation, immunodiffusion
Cross-reactivity	This antisera was specific to T18 antigen and did not cross-react with L44 antigen and T27 antigen
Proposed epitopes	D-glycero-D-galacto-heptose constitutes part of the immunodeterminant of the antigenic polysaccharide
IEDB Epitope	N/A
Proposed Utility	The polysaccharide antigen from strain T18 is distinct from those previously reported for this species and may be useful in serotyping E. saburreum
Curator ID	AA + AS
Date of Curation	28-05-2011
References	PMC260431

Entry No. - 37 [TOP]

PolysacDB ID	2495
Carbohydrate Name	O-polysaccharide [Terminal hexasaccharides] (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Vibrio cholerae O1 LPS serotype ogawa (NCBI Taxonomy) (Drugpedia)
Basic Structure	The O-antigen part consists of (1-->2)-α-linked 4-amino-4,6-dideoxy-D-mannose (perosamine) whose amino group is acylated with 3-deoxy-L-glycero-tetronic acid. The terminal sugar is characterized by a 2-O-methyl group
BCSDB Structure	6642
Proposed functions	V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugation Method	The purified carbohydrates were subjected to aminolysis to afford ethylenediamine derivatives, which were treated with squaric acid diethyl ester. The squaric acid monoesters, thus obtained, were treated with BSA to give neoglycoconjugates
Antibodies	Antisera
Antibody type and class	IgG and IgM
Assay System	ELISA
Cross-reactivity	N/A
Proposed epitopes	The epitope was named as Epitope A. Epitope A was postulated to be either the perosamine residues or the N-tetronic acid (N-3-deoxy-L-glycero-tetronic acid) side chain
IEDB Epitope	76824
Proposed Utility	This antisera showed protection
Curator ID	AA + AS
Date of Curation	04-06-2011
References	11920320

Entry No. - 38 [TOP]

PolysacDB ID	2496
Carbohydrate Name	O-polysaccharide [Terminal disaccharide] (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Vibrio cholerae O1 LPS serotype Inaba (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure	6642
Proposed functions	V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugation Method	The purified carbohydrates were subjected to aminolysis to afford ethylenediamine derivatives, which were treated with squaric acid diethyl ester. The squaric acid monoesters, thus obtained, were treated with BSA to give neoglycoconjugates
Antibodies	Antisera
Antibody type and class	IgM and IgG1
Assay System	ELISA
Cross-reactivity	This antisera cross-reacted with V. cholerae Ogawa and Inaba LPS
Proposed epitopes	N/A
IEDB Epitope	76775
Proposed Utility	This antisera was neither vibriocidal nor protective in the infant mouse cholera model
Curator ID	AA + AS
Date of Curation	05-06-2011
References	PMC427411

Entry No. - 39 [TOP]

PolysacDB ID	2497
Carbohydrate Name	O-polysaccharide [Terminal trisaccharide] (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Vibrio cholerae O1 LPS serotype Inaba (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure	6642
Proposed functions	V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugation Method	The purified carbohydrates were subjected to aminolysis to afford ethylenediamine derivatives, which were treated with squaric acid diethyl ester. The squaric acid monoesters, thus obtained, were treated with BSA to give neoglycoconjugates
Antibodies	Antisera
Antibody type and class	IgM and IgG2
Assay System	ELISA
Cross-reactivity	This antisera cross-reacted with V. cholerae Ogawa and Inaba LPS
Proposed epitopes	N/A
IEDB Epitope	76775
Proposed Utility	N/A
Curator ID	AA + AS
Date of Curation	05-06-2011
References	PMC427411

Entry No. - 40 [TOP]

PolysacDB ID	2498
Carbohydrate Name	O-polysaccharide [Terminal tetrasaccharide] (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Vibrio cholerae O1 LPS serotype Inaba (NCBI Taxonomy) (Drugpedia)
Basic Structure	It is a linear unbranched homopolymer of 1,6-linked-D-galactopyranosyl residues.
BCSDB Structure	6642
Proposed functions	V. cholerae lipopolysaccharide (LPS), a critical component of the outer membrane that is required for virulence, is a known target for immune responses following infection or immunization. Antibodies specific for V. cholerae LPS are correlated with protection against cholera
Antigenic Nature used to produce antibodies	Glycoconjugates
Carrier Name	Bovine serum albumin
Conjugation Method	The purified carbohydrates were subjected to aminolysis to afford ethylenediamine derivatives, which were treated with squaric acid diethyl ester. The squaric acid monoesters, thus obtained, were treated with BSA to give neoglycoconjugates
Antibodies	Antisera
Antibody type and class	IgM and IgG3
Assay System	ELISA, invivo protection assay
Cross-reactivity	This antisera cross-reacted with V. cholerae Ogawa and Inaba LPS
Proposed epitopes	N/A
IEDB Epitope	76775
Proposed Utility	This antisera helped in the epitope studies of O1 LPS
Curator ID	AA + AS
Date of Curation	05-06-2011
References	PMC427411

Entry No. - 41 [TOP]

PolysacDB ID	2502
Carbohydrate Name	Lipopolysaccharide (Drugpedia)
Carbohydrate Class	Lipopolysaccharide
Microbe	Vibrio cholerae Non-O1 serogroup (NCBI Taxonomy) (Drugpedia)
Basic Structure	N/A
BCSDB Structure	N/A
Proposed functions	N/A
Antigenic Nature used to produce antibodies	Whole cell bacteria and purified LPS
Carrier Name	Nil
Conjugation Method	Nil
Antibodies	Antisera
Antibody type and class	N/A
Assay System	ELISA
Cross-reactivity	This antisera was specific to V. cholerae had low cross reactivity with V.cholerae O1, serotype Inaba or Ogawa
Proposed epitopes	N/A
IEDB Epitope	N/A
Proposed Utility	This antisera can be used for the detection of Vibrio cholerae non O1 strrains
Curator ID	AA + AS
Date of Curation	08-06-2011
References	TUMS Journals

Department of Computational Biology, Indraprastha Institute of Information Technology, Sec - 39A, New Delhi, India - 110020