Help

The input sequence for prediction is a one-letter code nucleotide sequence in fasta format. The sequence can be pasted in the text area provided or can be uploaded through a file in the fasta format specified in the form.

Polyapred gives best result when input sequence having minimum length of 206 nucleotide having PAS like pattern in the middle.The program does not make prediction for sequence having shorter than 100 nt on both side of A[A/T]AAA. In such case only the position and the sequence of pattern will be displayed.

The threshold is used to discriminate the Polyadenylation signal(PAS) from non-PAS. The user can vary the threshold score between -1.0 to 1.0. The nucleotides achieving score more then the cutoff score are predicted as PAS otherwise they are predicted as non-PAS.if the user did not select any cutoff score then the default threshold of prediction methods will be used.

The results of the prediction displayed in user-freindly tabular formats. Each of result display format firstly provides a total number of positive and negative sites found followed by compehensive account of sequence name, length of input sequence, position of Polyadenylation signal, sequence of polyadenylation signal, score and the prediction result.