| DB ID | MyCo_7156 |
| Title | Beauveria attenuates asthma by inhibiting inflammatory response and inducing lymphocytic cell apoptosis |
| Year | 2016 |
| PMID | 27801673 |
| Fungal Diseases involved | Beauveria (BEA) |
| Associated Medical Condition | Phorbol-12-myristate-13-acetate (PMA) induced asthma |
| Genus | None |
| Species | None |
| Organism | None |
| Ethical Statement | This study was approved by the Ethics Committee of Jiangsu Drug Research Institute. All animal experiments followed the guideline of On the Care and Use of Laboratory Animals of the Institute. |
| Site of Infection | Lungs |
| Opportunistic invasive | Opportunistic |
| Sample type | Biopsy |
| Sample source | Lung Tissue Homogenate |
| Host Group | Animal |
| Host Common name | Mice |
| Host Scientific name | Mus musculus |
| Biomarker Name | Bax |
| Biomarker Full Name | Bcl-2-associated protein x |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | China |
| Cohort | Mesenteric lymph nodes of adult BALB/c mice were isolated under sterilized condition, placed in RPMI- 1640 culture medium (Hyclone, Logan, UT), minced gently and beat up with pipette tips to disperse cells. Then cells were filtered with 37 μm diameter filter screen (BD, NJ) followed by centrifugation at the speed of 1000rpm for 5min at 4 °C. Finally, the supernatant was discarded and cells were suspended in RPMI-1640 culture medium and cultured at a 37 °C incubator which was supplemented with 5% CO2. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Asthma is a common chronic inflammatory disease in the respiratory tract characterized by variable/ recurring symptoms of reversible airflow obstruction and bronchospasm. It is caused by a combination of genetic and environmental factors. The inflammaroty cells such as lymphocytes, macrophages and neutrophils are involved in the pathogenesis of asthma. Components of the immune system mediators including cytokines, chemokines, histamine and leukotrienes are contributing factors of asthma. The incidence of asthma has increased greatly since the 1970s. In 2011, it was diagnosed in 235-300 million people and caused 250,000 deaths globally. Despite the various treatment options currently available for the therapeutic management of asthma, a large number of patients with asthma continue to have poorly controlled disease. There is therefore a need of novel medication to achieve better asthma control. Beauveria (BEA) is a genus of asexually-reproducing fungi belongs to the ascomycete family cordycipitaceae. The role of BEA in inhibiting inflammation was previously reported in CD-1 mice. Many plant species containing flavonoids has been widely used as traditional Chinese medicine. Naringin, which possesses antioxidant, anti-inflammatory and anti-carcinogenic properties, was previously reported to protect ovalbumin-induced airway inflammation in asthma. Moreover, naringin was found to attenuate ovalbumin induced cough-variant asthma in a guinea pig model. As inflammation and its relationship to asthma are becoming better understood, the importance of anti-inflammatory treatment is increasingly accepted. However, whether BEA could be a medication for asthma is not clear. The present study focuses to investigate the effect of BEA on the growth of inflammatory cells and secretion of inflammatory mediators. The aim is to investigate if BEA plays a protective role in asthma. |
| Technique | Analytic |
| Analysis Method | western blot analysis |
| ELISA kits | ELISA kits Elabscience Biotechnology Co. Ltd, (Wuhan, China) |
| Assay Data | None |
| Validation Techniques used | ELISA, Western Blotting |
| Up Regulation Down Regulation | Increase |
| Sequence Data | None |
| External Link | None |
