| DB ID | MyCo_5744 |
| Title | Quick and accurate detection of Fusarium oxysporum f. sp. carthami in host tissue and soil using conventional and real-time PCR assay |
| Year | 2018 |
| PMID | 30446834 |
| Fungal Diseases involved | Safflower wilt |
| Associated Medical Condition | None |
| Genus | Fusarium |
| Species | oxysporum |
| Organism | Fusarium oxysporum f. spp. carthami (Foc) |
| Ethical Statement | None |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Plant extracts |
| Sample source | Extracted DNA |
| Host Group | Plant |
| Host Common name | Safflower |
| Host Scientific name | Carthamus tinctorius |
| Biomarker Name | FocScF/FocScR |
| Biomarker Full Name | FocScF/FocScR |
| Biomarker Type | Diagnostic |
| Biomolecule | Gene |
| Geographical Location | India |
| Cohort | One hundred twenty-four fungal isolates were used in this study. Seventy-five Foc isolates were used, of which 15 isolates were procured from Indian Agricultural Research Institute, India (IARI) and National Bureau of Agriculturally Important Microorganisms, India (NBAIM), while 60 isolates were collected from safflower growing regions in India during 2015–2017 cropping seasons. |
| Cohort No. | 124 |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Safflower is one of the important oilseed crops and is grown in more than 60 countries worldwide. It is a multipurpose crop used for its seeds (oil), flowers and foliage with several different uses. Safflower oil is a rich source of polyunsaturated fatty acids that play a vital role in reducing blood cholesterol levels. However, the sustainable production of safflower is limited by several biotic and abiotic stresses. Fusarium oxysporum Schlecht. f. sp. Carthami Klisiewicz and Houston is one of the most economically important diseases of safflower. Fusarium oxysporum f. sp. carthami (Foc) is a soil and seed-borne pathogen that causes wilt on safflower. The pathogen was initially described in California, USA, and later from other countries of the world, including India, Egypt, and Japan. Fusarium wilt has become the primary limiting factor for safflower production in India that has resulted in loss of her position as the major producer of safflower in the world. |
| Technique | PCR |
| Analysis Method | qRT-PCR |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | qRT-PCR |
| Up Regulation Down Regulation | Positive |
| Sequence Data | GenBank accession number MH667316 |
| External Link | None |
