MycoBiomDB – Record Details (MyCo_5743)

Biomarker Record Details

Database ID: MyCo_5743
DB IDMyCo_5743
TitleIn planta and soil quantification of Fusarium oxysporum f. sp. ciceris and evaluation of Fusarium wilt resistance in chickpea with a newly developed quantitative polymerase chain reaction assay
Year2011
PMID21219129
Fungal Diseases involvedFusarium wilt disease
Associated Medical ConditionNone
GenusFusarium
Speciesoxysporum
OrganismFusarium oxysporum f. spp. ciceris
Ethical StatementNone
Site of InfectionNone
Opportunistic invasiveNone
Sample typePlant extracts
Sample sourceExtracted DNA
Host GroupPlant
Host Common nameChickpea
Host Scientific nameCicer arietinum L.
Biomarker NameITS
Biomarker Full NameInternal Transcribed Spacer
Biomarker TypeDiagnostic
BiomoleculeGene
Geographical LocationSpain
CohortChickpea cvs. 12071/10054 (PV-1), PV-60, BG-212, P-2245, ICCV-2, UC-15, JG-62, CA334.20.4, ICC14216, PV61, WR-315, and CPS-1 were used in the study.
Cohort No.None
Age GroupNone
P ValueNone
SensitivityNone
SpecificityNone
Positive Predictive ValueNone
MICNone
Fold ChangeNone
PathwayNone
Disease Introduction MechanismChickpea (Cicer arietinum L.) is one of the world’s most important pulse crops with dry beans (Phaseolus vulgaris L.) and dry peas (Pisum sativum L.). It is not only a source of human food and animal feed but it also helps in the management of soil fertility, particularly in dry lands and the semiarid tropics where chickpea production is concentrated. Fusarium wilt caused by Fusarium oxysporum Schlechtend. Fr. f. sp. ciceris (Padwick) Matuo & K. Sato is the most important soilborne disease limiting chickpea production worldwide, but mainly in the Mediterranean Basin and the Indian subcontinent. Annual yield losses from this disease have been estimated to range from 10 to 15%. However, Fusarium wilt epidemics can be devastating to individual crops and cause 100% loss under disease-favorable conditions. In particular, disease attacks are devastat- ing if they occur when the crop is under heat and water stresses during the reproductive and seed filling stages.
TechniquePCR
Analysis MethodqRT-PCR
ELISA kitsNone
Assay DataNone
Validation Techniques usedqRT-PCR
Up Regulation Down RegulationPositive
Sequence DataNone
External LinkNone