| DB ID | MyCo_5743 |
| Title | In planta and soil quantification of Fusarium oxysporum f. sp. ciceris and evaluation of Fusarium wilt resistance in chickpea with a newly developed quantitative polymerase chain reaction assay |
| Year | 2011 |
| PMID | 21219129 |
| Fungal Diseases involved | Fusarium wilt disease |
| Associated Medical Condition | None |
| Genus | Fusarium |
| Species | oxysporum |
| Organism | Fusarium oxysporum f. spp. ciceris |
| Ethical Statement | None |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Plant extracts |
| Sample source | Extracted DNA |
| Host Group | Plant |
| Host Common name | Chickpea |
| Host Scientific name | Cicer arietinum L. |
| Biomarker Name | ITS |
| Biomarker Full Name | Internal Transcribed Spacer |
| Biomarker Type | Diagnostic |
| Biomolecule | Gene |
| Geographical Location | Spain |
| Cohort | Chickpea cvs. 12071/10054 (PV-1), PV-60, BG-212, P-2245, ICCV-2, UC-15, JG-62, CA334.20.4, ICC14216, PV61, WR-315, and CPS-1 were used in the study. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Chickpea (Cicer arietinum L.) is one of the world’s most important pulse crops with dry beans (Phaseolus vulgaris L.) and dry peas (Pisum sativum L.). It is not only a source of human food and animal feed but it also helps in the management of soil fertility, particularly in dry lands and the semiarid tropics where chickpea production is concentrated. Fusarium wilt caused by Fusarium oxysporum Schlechtend. Fr. f. sp. ciceris (Padwick) Matuo & K. Sato is the most important soilborne disease limiting chickpea production worldwide, but mainly in the Mediterranean Basin and the Indian subcontinent. Annual yield losses from this disease have been estimated to range from 10 to 15%. However, Fusarium wilt epidemics can be devastating to individual crops and cause 100% loss under disease-favorable conditions. In particular, disease attacks are devastat- ing if they occur when the crop is under heat and water stresses during the reproductive and seed filling stages. |
| Technique | PCR |
| Analysis Method | qRT-PCR |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | qRT-PCR |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |