| DB ID | MyCo_5742 |
| Title | New Molecular Markers Distinguishing Fonsecaea Agents of Chromoblastomycosis |
| Year | 2019 |
| PMID | 31317385 |
| Fungal Diseases involved | Chromoblastomycosis |
| Associated Medical Condition | None |
| Genus | Fonsecaea |
| Species | pugnacius |
| Organism | Fonsecaea pugnacius |
| Ethical Statement | All applicable international, national, and/ or institutional guidelines for the care and use of animals were followed (Ethics Committee for Animal Use from the Biological Sciences Section of the Federal University of Parana (CEUA/ BIO UFPR) with certified number 1179). |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Biopsy |
| Sample source | Extracted Lungs tissue |
| Host Group | Animal |
| Host Common name | Mice |
| Host Scientific name | Mus musculus |
| Biomarker Name | CBF5 |
| Biomarker Full Name | CBF5 |
| Biomarker Type | Diagnostic |
| Biomolecule | Gene |
| Geographical Location | Brazil |
| Cohort | An infection model was developed in immunocom- petent 6–8-week-old male BALB/c mice infected with F. pugnacius |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Human chromoblastomycosis (CBM) is a skin disease that is exclusively caused by the members of order Chaetothyriales (black yeasts and relatives). The infection occurs through inoculation of the pathogen by a trauma or injury caused by sharp natural materials, such as plant thorns or wooden splinters that carry the respective opportunistic pathogen. The agents of the disease may be morphologically indistinguishable from their environmental counterparts. The disease is one of the most prevalent implantation mycoses in the world and occurs particularly in the (sub) tropical regions of South America, Africa, and China. |
| Technique | PCR |
| Analysis Method | qRT-PCR |
| ELISA kits | enzyme‐linked immuno- sorbent assay Kit (ImmunoCAP ECP), NIOX MINO Kit (Aerocrine, Sweden) |
| Assay Data | None |
| Validation Techniques used | qRT-PCR |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
