| DB ID | MyCo_5727 |
| Title | MiR-30c-5p mediates inflammatory responses and promotes microglia survival by targeting eIF2α during Cryptococcus neoformans infection |
| Year | 2020 |
| PMID | 31958475 |
| Fungal Diseases involved | Cryptococcosis |
| Associated Medical Condition | None |
| Genus | Cryptococcus |
| Species | neoformans |
| Organism | Cryptococcus neoformans |
| Ethical Statement | This study was approved by Institutional review board (Committee on Ethics of Biomedicine, Second Military Medical University) and written informed consent (include written informed consent obtained from the parent(s) of patients under the age of 18 years) was obtained before patients and healthy volunteers were entered into this study according to the Declaration of Helsinki. |
| Site of Infection | None |
| Opportunistic invasive | Invasive |
| Sample type | Body fluid |
| Sample source | Cerebrospinal fluid (CSF) |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | miR-30c-5p |
| Biomarker Full Name | miR-30c-5p |
| Biomarker Type | Diagnostic |
| Biomolecule | miRNA |
| Geographical Location | China |
| Cohort | Cerebrospinal fluid(CSF)andserumsampleswereobtainedfrom11patients with cryptococcal meningitis(7males and 4 females; age range 16–62 years, mean 45 years old, all patients are HIV(−); CSF and serum were collected before and after effective antifungal therapy.) All of the patients fulfilled the criteria of the Infectious Diseases Society of America (IDSA). Control serum samples were also collected from 12 age- and sex-matched healthy volunteers. |
| Cohort No. | 11 |
| Age Group | 16-62 |
| P Value | p< 0.04 |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Cryptococcosis is a globally distributed invasive fungal infectious disease that is caused by Cryptococcus neoformans and Cryptococcus gattii. Cryptococcosis has gradually increased worldwide in the last 20 years, especially in China. The most common risk factor for cryptococcosis caused by C. neoformans is immunodeficiency disease, such as AIDS, while infections caused by C. gattii are often reported in immunocompetent patients. Epidemiological studies from Far East Asian countries, especially from China, show that C. neoformans infects mostlyHIV-negativepatients,andtheseso-calledhealthypeoplehavea longer course of treatment. Subtle defects in the innate immune response to fungi may cause at least some cases of C. neoformans infection in healthy individuals. |
| Technique | PCR |
| Analysis Method | qRT-PCR |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | qRT-PCR, RNAseq , flow cytometry, immunoblotting, luciferase assay and immunohistochemistry |
| Up Regulation Down Regulation | Up regulated |
| Sequence Data | None |
| External Link | None |
