MycoBiomDB – Record Details (MyCo_5526)

Biomarker Record Details

Database ID: MyCo_5526
DB IDMyCo_5526
TitleDistribution of mating-type alleles and M13 PCR markers in the black leaf spot fungus Mycosphaerella fijiensis of bananas in Brazil
Year2013
PMID23420369
Fungal Diseases involvedBlack sigatoka
Associated Medical ConditionNone
GenusMycosphaerella
Speciesfijiensis
OrganismMycosphaerella fijiensis
Ethical StatementNone
Site of InfectionNone
Opportunistic invasiveNone
Sample typePlant extracts
Sample sourceExtracted DNA
Host GroupPlant
Host Common nameBanana
Host Scientific nameMusa paradisiaca
Biomarker NameM13
Biomarker Full NameM13
Biomarker TypeDiagnostic
BiomoleculeGene
Geographical LocationBrazil
CohortThese studies were performed at the Laboratórios de Fitopatologia e Biologia Mole- cular da Embrapa da Amazônia Ocidental, Amazonas, Brazil. The M. fijiensis isolates were obtained from banana leaves showing disease symptoms and maintained using potato dextrose agar media at 27°C.
Cohort No.None
Age GroupNone
P ValueNone
SensitivityNone
SpecificityNone
Positive Predictive ValueNone
MICNone
Fold ChangeNone
PathwayNone
Disease Introduction MechanismBananas are one of the most consumed fruits in the world, and Brazil ranks fourth in terms of banana production (FAO, 2010). Biological stress is one of the major limiting factors for the major banana-producing areas. The disease black sigatoka is caused by the fungus My- cosphaerella fijiensis Morelet and is particularly harmful to bananas. This fungus causes the premature death of leaves, thus reducing the photosynthetic ability of the plant and causing the production of irregular fruit with no commercial value. Black siga- toka was detected in Brazil in 1998 in the municipalities of Benjamin Constant and Tabatinga in the State of Amazonas. The disease has since spread throughout the North, South, Southeast, and Central-West regions, with the exception of the States of Espírito Santo, Rio de Janeiro, Goiás, and the Federal District.
TechniquePCR
Analysis MethodPCR Based
ELISA kitsNone
Assay DataNone
Validation Techniques usedPCR
Up Regulation Down RegulationPositive
Sequence DataNone
External LinkNone