| DB ID | MyCo_5417 |
| Title | Invariant Natural Killer T Cells as Key Players in Host Resistance against Paracoccidioides brasiliensis |
| Year | 2021 |
| PMID | 33954206 |
| Fungal Diseases involved | Paracoccidioidomycosis |
| Associated Medical Condition | None |
| Genus | Paracoccidioides |
| Species | brasiliensis |
| Organism | Paracoccidioides brasiliensis |
| Ethical Statement | All procedures were previ- ously reviewed and approved by the internal ethics committee of Universidade de São Paulo (USP-180/2011) and Universi-dade Federal de São Paulo (UNIFESP–CEP 0372/12). |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Body fluid |
| Sample source | Bronchoalveolar lavage fluid (BALF) |
| Host Group | Animal |
| Host Common name | Mice |
| Host Scientific name | Mus musculus |
| Biomarker Name | NO |
| Biomarker Full Name | Nitric Oxide |
| Biomarker Type | Diagnostic |
| Biomolecule | None |
| Geographical Location | Brazil |
| Cohort | Animals. Isogenic male C57Bl/6 mice from wild-type (WT), CD1d-/-, and iNKT-deficient strain (Jα18-/-), aged 8– 12 weeks, were obtained from the Animal Care Facility at the Federal University of São Paulo (UNIFESP). The C57Bl/ 6 Jα18-/- strain was a gift from Dr. Masaru Taniguchi at the RIKEN Research Center for Allergy and Immunology (Japan) |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Paracoccidioidomycosis (PCM) is caused by a fungus from the Paracoccidioides genus and is considered one of the high-est causes of mortality among Brazilian systemic mycoses. Clinical studies demonstrated a relationship between the characteristics of the immune response and disease severity. In humans, a prominent Th1 response is associated with infection without disease, the chronic form of the disease with Th1/Th17 immunity, and the most severe manifesta-tion, the acute or juvenile form, shows a prominent Th2/Th9 profile. These data are supported by murine models of P. brasiliensis infection showing the association between the classical Th1 immune response, with high levels of IL-2 and IFN-γ, with resistance against the fungi. |
| Technique | Assay |
| Analysis Method | Nitrate/Nitrite Colorimetric Assay |
| ELISA kits | ELISA (R&D Systems, USA). |
| Assay Data | Nitrate/Nitrite Colorimetric Assay (Cayman Chemicals, USA) |
| Validation Techniques used | ELISA, Nitrate/Nitrite Colorimetric Assay, Flow Cytometry Analysis, multiplex analysis |
| Up Regulation Down Regulation | Decrease |
| Sequence Data | None |
| External Link | None |
