| DB ID | MyCo_5227 |
| Title | Distinguish metabolic profiles and defense enzymes in Alternaria leaf blight resistant and susceptible genotypes of groundnut |
| Year | 2019 |
| PMID | 31736543 |
| Fungal Diseases involved | Alternaria leaf blight |
| Associated Medical Condition | None |
| Genus | Alternaria |
| Species | alternata |
| Organism | Alternaria alternata |
| Ethical Statement | None |
| Site of Infection | Leaves |
| Opportunistic invasive | None |
| Sample type | Plant extracts |
| Sample source | Plant extracts |
| Host Group | Plant |
| Host Common name | Penaut |
| Host Scientific name | Arachis hypogaea |
| Biomarker Name | Fructose |
| Biomarker Full Name | Fructose |
| Biomarker Type | Diagnostic |
| Biomolecule | Metabolite |
| Geographical Location | India |
| Cohort | The seeds of groundnut genotypes CS186 and GPBD4 (Alterneria resistant), GG2 and TPG41 (Susceptible) were procured from Plant Breeding Section, ICAR-Directorate of Groundnut Research, Junagadh, Gujarat, India. All the genotypes were raised in earthen pots under PII glass house to maintain uniform temperature and humidity during summer 2015. A potting mixture (Vertisol, sand and farm yard manure in a 2:1:1 ratio) with diammonium phosphate at 1 g kg-1 of soil was used. Each genotype was grown in six pots; three pots were kept for control and three for inoculation of Alterneria pathogen (i.e. two sets). Four plants were kept in each pot (20 kg soil capacity). The pathogen, Alternaria alternata (Fr.) Keissler isolated from field grown infected leaves of TPG41. Pathogen was cultured on potato dextrose agar (PDA) as a pure culture. The inoculum was sprayed on leaves in evening at 40 DAS. Leaf samples were taken 5 days after infection (dai) because conidial germination and successful infection of A. alternate occurs at 48–72 h after inoculation of spores and development of disease symptoms starts 5 days after inoculation. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Alternaria leaf blight is becoming a major fungal disease of summer groundnut. Outbreak of this minor disease was observed in farmer’s fields in summer groundnut during 2009 in Saurashtra and Kuchchh regions of Gujarat, India. The main effect of the disease is defoliation of plants, thereby causing reduction in pod yield from 13 to 22% and the reduction in haulm yield from 24 to 63%. Groundnut crop residues are widely used for feeding dairy animals. Leaf diseases are likely to affect the quantity and nutritive value of the crop residues, resulting in reductions in milk production. Furthermore, residues fetch low prices in the fodder markets. Being considered a minor disease, very less studies are reported. |
| Technique | Gas chromatography |
| Analysis Method | Metabolomics Approach |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | Non-targeted metabolite profiling using GC-MS |
| Up Regulation Down Regulation | Decrease |
| Sequence Data | None |
| External Link | None |
