| DB ID | MyCo_5201 |
| Title | Verticillium longisporum infection affects the leaf apoplastic proteome, metabolome, and cell wall properties in Arabidopsis thaliana |
| Year | 2012 |
| PMID | 22363647 |
| Fungal Diseases involved | Verticillium longisporum infection |
| Associated Medical Condition | None |
| Genus | Verticillium |
| Species | longisporum |
| Organism | Verticillium longisporum |
| Ethical Statement | None |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Plant extracts |
| Sample source | Plant extracts |
| Host Group | Plant |
| Host Common name | Arabidopsis thaliana |
| Host Scientific name | Arabidopsis thaliana |
| Biomarker Name | Pinoresinol diglucoside |
| Biomarker Full Name | Pinoresinol diglucoside |
| Biomarker Type | Diagnostic |
| Biomolecule | Metabolite |
| Geographical Location | Germany |
| Cohort | Arabidopsis thaliana (ecotype Columbia 0) were grown and inoculated with Verticillium longisporum as described previously. After germination on agar, Arabidopsis seedlings were removed, roots were wounded by cutting the tip, and plants were potted into soil (Typ T25, Fruhstorfer, Vechta, Germany) together with 26 107 spores of V. longisporum in 10 ml sterile water applied directly to the roots. Control plants were treated in the same way without spores. The plants were grown at 20uC, 60% relative air humidity, and 120 mmol m22 s21 photosynthetic active radiation (8 h light/16 h dark cycle), watered with tap water and fertilized once a week with Wuxal (Aglucon, Du ¨ sseldorf, Germany). |
| Cohort No. | None |
| Age Group | None |
| P Value | p=4.60E-12 |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | 7.4 fold |
| Pathway | None |
| Disease Introduction Mechanism | Verticillium species are wide-spread soil borne fungi, which cause vascular diseases in many plant species. Most studies addressed plant responses to V. dahliae or V. albo-atrum, which have the largest host range and cause billions of dollars of yield loss in crops worldwide. In addition, V. longisporum (VL) infection has been identified in the last decade as one of the most important diseases of Brassicaceae, in particular of oilseed rape. To date, the economic importance and acreage of oilseed rape are increasing because of growing demand on oil crops for nutrition and bio-fuels. This is accompanied by a spread of VL diseases, which may cause yield losses as high as 10 to 50%. |
| Technique | Liquid chromatography |
| Analysis Method | Metabolomics Approach |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | 2D gel electrophoresis, mass spectrometry, qRT-PCR, FTIR, Metabolomics |
| Up Regulation Down Regulation | Induced |
| Sequence Data | None |
| External Link | None |
