| DB ID | MyCo_5114 |
| Title | RsmA regulates Aspergillus fumigatus gliotoxin cluster metabolites including cyclo(L-Phe-L-Ser), a potential new diagnostic marker for invasive aspergillosis |
| Year | 2013 |
| PMID | 23671611 |
| Fungal Diseases involved | Invasive aspergillosis |
| Disease | Aspergillosis (Aspergillus Infection) |
| Associated Medical Condition | None |
| Genus | Aspergillus |
| Species | fumigatus |
| Organism | Aspergillus fumigatus |
| Ethical Statement | Neutrophils were obtained from whole blood of self-reportedly healthy donors, from which we obtained informed and written consent at the time of the blood draw with approval of the University of Wisconsin-Madison Center for Health Sciences Human Subjects committee. The Keller animal protocol number is M02468 as approved by the University of Wisconsin-Madison Research Animal Resources Center. |
| Site of Infection | Lungs |
| Opportunistic invasive | Invasive |
| Sample type | Biopsy |
| Sample source | Extracted lungs tissue |
| Host Group | Animal |
| Host Common name | Mice |
| Host Scientific name | Mus musculus |
| Biomarker Name | Yap-like bZIP termed RsmA (restorer of secondary metabolism A) |
| Biomarker Full Name | Yap-like Dimeric basic leucine zipper termed RsmA (restorer of secondary metabolism A) |
| Biomarker Type | Diagnostic |
| Biomolecule | Metabolite |
| Geographical Location | USA |
| Cohort | A. fumigatus wild type and OErsmA strains were assessed in a lung infection model of invasive aspergillosis. Briefly, female Swiss ICR mice (Harlan Sprague Dawley) weighing about 18–20 g were immunosuppressed via administration of cyclophosphamide by separate intraperitoneal injections, one at 4 days (200 mg/kg of body weight) and the other at 1 day (200 mg/kg) before infection. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Aspergillus fumigatus is an opportunistic pathogen of immunosuppressed individuals. In immunosuppressed hosts, invasive aspergillosis (IA) represents a major cause of morbidity and mortality, with mortality rates ranging from 40% to 90%. Part of the virulence of A. fumigatus is associated with its ability to produce a variety of toxic natural products. The natural product pathways in fungi result in the production of structurally diverse and low- molecular-weight compounds referred to as secondary metabolites (SMs). Although the functions of many fungal SMs remain to be determined, the known SMs of the genus Aspergillus have a tremendous impact on society. |
| Sub Technique | LC-MS + Computational |
| Technique | Hybrid Methods |
| Analysis Method | Metabolomics Approach |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | HPLC-MS |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
