| DB ID | MyCo_3815 |
| Title | Gliotoxin and bis(methylthio)gliotoxin are not reliable as biomarkers of invasive aspergillosis |
| Year | 2019 |
| PMID | 31313395 |
| Fungal Diseases involved | Invasive pulmonary aspergillosis |
| Associated Medical Condition | Haematology Patients |
| Genus | Aspergillus |
| Species | fumigatus |
| Organism | Aspergillus fumigatus |
| Ethical Statement | The authors confirm that the ethical policies of the journal, as noted on the journal's author guidelines page, have been adhered to and the appropriate ethical review committee approval has been received (ClinicalTrials.gov NCT03004092). |
| Site of Infection | None |
| Opportunistic invasive | Invasive |
| Sample type | Body fluid |
| Sample source | Serum |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | bmGT |
| Biomarker Full Name | Bis(methylthio)gliotoxin (bmGT) |
| Biomarker Type | Diagnostic |
| Biomolecule | Metabolite |
| Geographical Location | Belgium |
| Cohort | Here tested prospectively collected fungal cultures, BALf and serum of consecutive patients with culture‐positive IPA for the presence of GT and bmGT. A colony from each culture was transferred to 10 mL of Sabouraud liquid medium and cultured at 37°C for 72 hours. Here included 18 patients with proven (n=6) and probable (n=12) IPA, all with positive cultures for Aspergillus fumigatus. |
| Cohort No. | 18 |
| Age Group | None |
| P Value | p=0.002 |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Invasive aspergillosis (IA) remains a life‐threatening opportunistic infection, especially in patients with acute leukaemia or after haematopoietic cell transplantation.1 Rapid and accurate diagnosis is essential for the timely initiation of adequate mould‐active therapy. Currently, several diagnostic tools are used, including methods that directly detect the causative Aspergillus species such as fungal culture and direct microscopy (preferentially using optical brighteners). However, these diagnostic techniques suffer from low sensitivity. |
| Technique | Liquid chromatography |
| Analysis Method | High‐performance liquid chromatography–quadrupole time of fligh mass spectrometry |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | High‐performance liquid chromatography–quadrupole time of fligh mass spectrometry |
| Up Regulation Down Regulation | Negative |
| Sequence Data | None |
| External Link | None |
