| DB ID | MyCo_3461 |
| Title | Susceptibility of A. fumigatus-specific T-cell assays to pre-analytic blood storage and PBMC cryopreservation greatly depends on readout platform and analytes |
| Year | 2018 |
| PMID | 29611226 |
| Fungal Diseases involved | Invasive aspergillosis |
| Disease | Aspergillosis (Aspergillus Infection) |
| Associated Medical Condition | None |
| Genus | Aspergillus |
| Species | fumigatus |
| Organism | Aspergillus fumigatus |
| Ethical Statement | This work and consent was approved by the ethics committee of the University of Wuerzburg. |
| Site of Infection | Unspecified |
| Opportunistic invasive | Opportunistic |
| Sample type | Biopsy |
| Sample source | Peripheral blood mononuclear cell (PBMC) |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | CD154 |
| Biomarker Full Name | cluster of differentiation 154 |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | Germany |
| Cohort | After obtaining written consent approved by the ethics committee of the University of Wuerzburg, 150 ml Li-Heparin blood and 15 ml serum were drawn from healthy adult donors. Subjects were screened for absence of acute or chronic infectious diseases, diabetes mellitus, pregnancy, immunosuppressive therapy or recent vaccinations. |
| Cohort No. | None |
| Age Group | None |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| Fold Change | 0.44 |
| Pathway | None |
| Disease Introduction Mechanism | None |
| Sub Technique | Flow cytometry |
| Technique | Cell-based Assay |
| Analysis Method | Flow Cytometry Analysis |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | ELISPOT, Flow Cytometry |
| Up Regulation Down Regulation | None |
| Sequence Data | None |
