| DB ID | MyCo_2688 |
| Title | Matrix metalloproteinases (MMP-8, MMP-9) and the tissue inhibitors of metalloproteinases (TIMP-1, TIMP-2) in patients with fungal keratitis |
| Year | 2007 |
| PMID | 17251814 |
| Fungal Diseases involved | Fungal keratitis |
| Associated Medical Condition | None |
| Genus | Aspergillus |
| Species | flavus |
| Organism | Aspergillus flavus |
| Ethical Statement | None |
| Site of Infection | Eye |
| Opportunistic invasive | Opportunistic |
| Sample type | Biopsy |
| Sample source | Corneal samples |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | TIMP-1 |
| Biomarker Full Name | Tissue Inhibitors of Metalloproteinase-1 |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | India |
| Cohort | This study had 2 broad aims: 1) study the infiltrating cells from the corneal buttons from a group of patients with culture-positive fungal keratitis (group 1), and 2) study the MMPs in the tear, serum, and corneal button in a different group of patients with culture-positive fungal keratitis (group 2) and determine their corresponding MMP-to-TIMP ratios. Four patients with fungal keratitis in group 1 provided tissues for the histopathologic study only. Ten patients with fungal keratitis in group 2 were chosen for tear, serum, and MMP studies. The patients with fungal keratitis included in this study were previously scheduled to undergo therapeutic keratoplasty (TPK) for reasons including perforation, impending perforation, or nonhealing ulcers, despite adequate and appropriate antifungal therapy. Corneal scrapings for smear examinations and culture were obtained from these patients before surgery, and samples from the infected buttons were also cultured. Corneal buttons of 5 patients with keratoconus scheduled to undergo penetrating keratoplasty (PKP) and 5 cadaver corneas served as controls. |
| Cohort No. | None |
| Age Group | 36-75 |
| P Value | None |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Fungal keratitis is increasingly reported as a significant cause of ocular morbidity in developing countries. In certain countries, fungi have replaced bacteria as the most common cause of suppurative keratitis. The visual outcomes of severe fungal keratitis are often poor, with 1 study reporting a 25% evisceration rate. Advances in antifungal therapy have not matched those of antibacterial medications, and this may be one of the significant reasons for the poorer visual outcomes. An intact epithelium with a healthy tear film protects the cornea from infection. Early lesions in infective keratitis confined to the corneal epithelium are amenable to treatment with topical antifungal medications wherein a rapid resurfacing of the affected area by the epithelial cells is noticed. |
| Technique | ELISA |
| Analysis Method | ELISA Based |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | ELISA |
| Up Regulation Down Regulation | Increase |
| Sequence Data | None |
| External Link | None |
