| DB ID | MyCo_2467 |
| Title | The role of bronchoalveolar lavage galactomannan in the diagnosis of pediatric invasive aspergillosis |
| Year | 2009 |
| PMID | 19238113 |
| Fungal Diseases involved | Invasive aspergillosis |
| Associated Medical Condition | None |
| Genus | Aspergillus |
| Species | spp. |
| Organism | Aspergillus spp. |
| Ethical Statement | ELISA Based |
| Site of Infection | None |
| Opportunistic invasive | Invasive |
| Sample type | Body fluid |
| Sample source | Bronchoalveolar lavage fluid (BALF) |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | GM |
| Biomarker Full Name | Galactomannan |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | USA |
| Cohort | Eighty-five BAL GM assays were submitted at the discre- tion of the attending clinicians. Of 85 pediatric patients who had a BAL GM submitted during the study, 59 were immunocompromised. Three patients were identified as having proven IA, 6 had probable IA, 37 had possible IA, and 39 had no evidence of IA; 38 had a concurrent serum GM performed. |
| Cohort No. | 85 |
| Age Group | None |
| P Value | None |
| Sensitivity | 0.78 |
| Specificity | 1 |
| Positive Predictive Value | 0.58 |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Invasive aspergillosis (IA) is an important cause of morbidity and mortality in immunocompromised pediatric patients. Timely di- agnosis is challenging because comorbid conditions and immuno- modulation often result in nonspecific signs and symptoms of disease. In addition, in pediatric patients, classic radiologic find- ings are identified at a lower incidence than in adults. Although the standard for diagnosis is lung biopsy, this approach engenders risk in medically fragile, neutropenic patients and has unclear sensitivity. Focus on early diagnosis has resulted in identifying surrogate markers such as galactomannan (GM), a cell wall poly- saccharide released by Aspergillus hyphae. |
| Technique | ELISA |
| Analysis Method | ELISA Based |
| ELISA kits | ELISA Kit- Platelia Aspergillus (Platelia Aspergillus EIA, Bio-Rad, France). |
| Assay Data | None |
| Validation Techniques used | FDA Approved -Platelia Aspergillus ELISA |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
