| DB ID | MyCo_1663 |
| Title | Evolution of CD4⁺CD25(hi) T cell subsets in Aspergillus-infected liver transplantation recipients reduces the incidence of transplantation rejection via upregulating the production of anti-inflammatory cytokines |
| Year | 2014 |
| PMID | 25062480 |
| Fungal Diseases involved | Aspergillosis |
| Associated Medical Condition | Aspergillus-infected liver transplant recipients |
| Genus | Aspergillus |
| Species | fumigatus |
| Organism | Aspergillus fumigatus |
| Ethical Statement | None |
| Site of Infection | None |
| Opportunistic invasive | Opportunistic |
| Sample type | Body fluid |
| Sample source | Serum |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | IL-4 |
| Biomarker Full Name | Interleukin-4 |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | China |
| Cohort | We examined data from 75 liver transplant recipients (67 male, 8 female; average age, 42.3 years) with or without histologically and polymerase chain reaction (PCR) confirmed as¬pergillosis after liver transplantation from March 1, 2010 to March 31, 2011 in our hospi¬tal. Clinical samples (sputum, blood, and liver tissue) were examined for fungal infections by microscopic examination and culture on Sabouraud dextrose agar (Merck; Germany). Blood samples were collected at 1-week intervals for molecular examinations. Sera from the patients were extracted for Aspergillus DNA detection using the QIAamp DNA Minikit (Qiagen; Ger¬many) in accordance with manufacturer recommendations. Nested PCR was performed with 2 sets of primers according to Yamakami et al. (1996). Based on comparison of the 18S rRNA sequence genes of Aspergillus, the Aspergillus species (panfungal aspergillosis) was identified. |
| Cohort No. | 75 |
| Age Group | 42.3 |
| P Value | p< 0.0001 |
| Sensitivity | None |
| Specificity | None |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Aspergillosis is caused by the spores of Aspergillus, which is ubiquitously distributed in the environment under certain conditions, such as in patients with immune suppressive therapy or in those who have undergone solid organ and stem cell transplantation, and reaches approximately 80% mortality. Of the nearly 185 Aspergillus species, A. fumigatus is the most prevalent pathogen, and accounts for more than 90% of all infections reported. The incidence of invasive aspergillosis occurs in 1 to 8% of liver transplant recipients and the mortality rate ranges from 60 to 80%. Aspergillosis can occur as a local or a systemic infection, depending primarily on the functions of helper T (Th) cell subsets and regulatory T (Treg) cells. Both Th1, Th2, and Th17 responses in mice were shown to evolve during repeated pulmonary exposure to Aspergillus. Treg cells involved in the development of aspergillosis provide the host with adequate defense without necessarily eliminating the fungus or causing unaccept¬able levels of host damage. |
| Technique | ELISA |
| Analysis Method | ELISA Based |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | ELISA, FACS,RT-PCR, Western Blot |
| Up Regulation Down Regulation | Increase |
| Sequence Data | None |
| External Link | None |
