| DB ID | MyCo_1516 |
| Title | Negative serum (1,3) -β-D-glucan has a low power to exclude Pneumocystis jirovecii pneumonia (PJP) in HIV-uninfected patients with positive qPCR |
| Year | 2023 |
| PMID | 37986091 |
| Fungal Diseases involved | Pneumocystis jirovecii pneumonia |
| Associated Medical Condition | Lung cancer |
| Genus | Pneumocystis |
| Species | jirovecii |
| Organism | Pneumocystis jirovecii |
| Ethical Statement | The Ethics Committee of Peking Union Medical College Hospital (K22C1279) reviewed and approved the study. |
| Site of Infection | None |
| Opportunistic invasive | None |
| Sample type | Body fluid |
| Sample source | Serum |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | BDG |
| Biomarker Full Name | 1-3-beta-D-Glucan |
| Biomarker Type | Diagnostic |
| Biomolecule | Protein |
| Geographical Location | China |
| Cohort | This study recruited 330 HIV-uninfected patients with P. jirovecii qPCR-positive results from Peking Union Medical College Hospital (PUMCH). Clinical and biological data of all the participants were collected. Only the first qPCR test during hospitalization for patients with multiple P. jirovecii qPCR test results was included for subsequent analyses. |
| Cohort No. | 330 |
| Age Group | None |
| P Value | p<0.0001 |
| Sensitivity | 0.5556 |
| Specificity | 1 |
| Positive Predictive Value | 1 |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Pneumocystis jirovecii (formerly Pneumocystis carinii) pneumonia (PJP), is a life-threatening fungal infection predominantly observed in immunocompromised individuals. HIV uninfected populations have underlying risk factors for immunodeficiency, including hematological malignancies, stem-cell or solid-organ transplants, autoimmune diseases, and solid cancers. They are at increased risk for developing PJP. More atypical clinical manifestations, faster disease progression, and a higher risk of respiratory failure than in HIV-infected patients characterizes PJP in HIV-uninfected patients. With the rising morbidity and mortality of PJP, timely and accurate diagnosis is essential. Therefore, it requires typical clinical features and microbiological confirmation. |
| Technique | ELISA |
| Analysis Method | ELISA Based |
| ELISA kits | (1–3)-ß-D Dextran Test Kit (Zhanjiang A&C, Guangdong, China). |
| Assay Data | None |
| Validation Techniques used | (1–3)-ß-D Dextran Test Elisa |
| Up Regulation Down Regulation | Increase |
| Sequence Data | None |
| External Link | None |
