| DB ID | MyCo_1054 |
| Title | Therapy with antifungals decreases the diagnostic performance of PCR for diagnosing invasive aspergillosis in bronchoalveolar lavage samples of patients with haematological malignancies |
| Year | 2012 |
| PMID | 22740590 |
| Fungal Diseases involved | Invasive aspergillosis |
| Associated Medical Condition | Hematological malignancies |
| Genus | Aspergillus |
| Species | spp. |
| Organism | Aspergillus spp. |
| Ethical Statement | None |
| Site of Infection | None |
| Opportunistic invasive | Invasive |
| Sample type | Body fluid |
| Sample source | Bronchoalveolar lavage fluid (BALF) |
| Host Group | Human |
| Host Common name | Human |
| Host Scientific name | Homo sapiens |
| Biomarker Name | 18S rRNA gene |
| Biomarker Full Name | 18S rRNA gene |
| Biomarker Type | Diagnostic |
| Biomolecule | Gene |
| Geographical Location | Germany |
| Cohort | BAL samples submitted to our laboratory between January 2000 and February 2011, initially processed to detect Aspergillus DNA by a nested PCR assay, were retrospectively analysed to elucidate the influence of antifungal therapy on PCR performance. Two-hundred-and-twenty-six BAL samples from 226 haematological patients at high risk for IA and pulmonary infiltrates were obtained between January 2000 and February 2011, and analysed by an Aspergillus-specific nested PCR assay. |
| Cohort No. | 226 |
| Age Group | 56 |
| P Value | p<0.009 |
| Sensitivity | 0.0069 |
| Specificity | 0.0087 |
| Positive Predictive Value | None |
| MIC | None |
| Fold Change | None |
| Pathway | None |
| Disease Introduction Mechanism | Invasive fungal infections (IFIs) are frequent and life-threatening infectious diseases in patients with severe immunosuppression, such as intensive, myeloablative chemotherapy or allogeneic stem cell transplantation. A significant proportion of IFIs consists of invasive mould infections, with invasive pulmonary aspergillosis (IPA) accounting for the majority of IFIs. Establishing the diagnosis of an IFI is based on criteria published by the European Organiza- tion for the Research and Treatment of Cancer/Mycoses Study Group (EORTC/MSG), initially published in 2002 and revised in 2008. |
| Technique | PCR |
| Analysis Method | Aspergillus PCR |
| ELISA kits | None |
| Assay Data | None |
| Validation Techniques used | Aspergillus PCR |
| Up Regulation Down Regulation | Positive |
| Sequence Data | None |
| External Link | None |
