| ID | 1541 | |
| PMID | 22386653 | |
| Year | 2012 | |
| Sequence | QNPASPPEEGSPDPDSTGA LVEEEDPFFKVPVNKLA AAVSNFGYDLYRVRSSTS PTTNVLLSPLSVATAL SALSLGAEQRTESIIHR ALYYDLISSPDIHGTYKEL LDTVTAPQKNLKSAS RIVFEKKLRIKSSFVAPLE KSYGTRPRVLTGNP RLDLQEINNWVQAQ MKGKLARSTKEIPDEIS ILLLGVAHFKGQWVTKF DSRKTSLEDFYLDEER TVRVPMMSDPKAVLR YGLDSD | |
| Name | PEDF (Pigment epithelium-derived factor) | |
| Length | 399 | |
| N-Terminal Modification | Pyrrolidone carboxylic acid | |
| C-Terminal Modification | Free | |
| Linear/ Cyclic | Linear | |
| Chirality | L | |
| Chemical Modification | Glycosylation at 266th residue, phosphoserine at 5, 95 and 108th residue | |
| Origin of Peptide | Neurotrophic protein | |
| Nature of Peptide/Cargo | It induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. | |
| Mechanism | Neurotrophic protein | |
| Cargo Sequence/Structure | None | |
| Name of cargo | Not applicable | |
| Assay | VEGF and 8-OHdG Immunostaining, Measurement of corneal neo-vascularization via avidin-biotin-alkaline phosphatase kit and statistical analysis | |
| Enhancer | None | |
| Properties of enhancer | Not applicable | |
| Concentration | 1 eye drop of 1 µM thrice a day for 7 days | |
| Incubation time | 7 Days | |
| Tissue permeability (value with units) | Neo-vascularized area= 22% due to PEDF (PBS=31%) or 65% of PBS on day 7(Control=4% of PBS); Oxidative stress generation, 8-OHdG level=75% of PBS (Control=50% of PBS); VEGF expression, VEGF level=71% of PBS (Control=40% of PBS) | |
| Tissue Sample | Seven week-old male Spraque–Dawley rat's right eye was used as test and left eye considered as control (PBS) | |
| Ex vivo/In vivo/In vitro | in vivo | |
| STRUCTURE |
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| SMILES | N.A. | |