| PRIMARY INFORMATION |
|---|
| ID | 1525 |
| PMID | 17923842 |
| Year | 2014 |
| Sequence | GGG(ARKKAAKA)4 |
| Name | L-POD (lissamine- conjugated peptide for ocular delivery) |
| Length | 35 |
| N-Terminal Modification | Conjugated to lissamine |
| C-Terminal Modification | Free |
| Linear/ Cyclic | Linear (Branched) |
| Chirality | L |
| Chemical Modification | None |
| Origin of Peptide | Novel peptide for ocular delivery (POD) |
| Nature of Peptide/Cargo | A novel cell-penetrating peptide POD has the ability to penetrate and deliver fluorophores, siRNA, DNA and quantum dots to cells in culture and retinal and ocular tissues in vivo. It also acts as a microbicide, potentially providing a safer and more efficacious method of delivering molecules t |
| Mechanism | Novel peptide for ocular delivery (POD) |
| Cargo Sequence/Structure | None |
Name of cargo
| Not applicable |
| Assay | Fluorescence-activated cell sorting (FACS), Vital staining with Lissamine |
| Enhancer | HER cells incubated with 2 nmol (8 μmol/l) L-POD for 15 minutes were treated with trypsin at 37 °C prior to counting of lissamine-positive cells by fluorescence-activated cell sorting (FACS) |
| Properties of enhancer | Not mentioned |
| Concentration | 10 nmol of L-POD |
| Incubation time | 45 minutes |
| Tissue permeability (value with units) | L-POD bound strongly to the cornea, sclera and the dura of the optic nerve in vivo. In contrast, lissamine only weakly stained the cornea and sclera and did not stain the dura of the optic nerve. |
| Tissue Sample | Cornea of male C57BL/6J mice |
| Ex vivo/In vivo/In vitro | in vivo |
| SECONDARY INFORMATION |
|---|
| STRUCTURE | |
| SMILES | N.A. |
