Welcome to Entry Card of Peptide


Details of TopicalPdb ID 1436

PRIMARY INFORMATION
ID1436
PMID2842178
Year1988
SequenceACDTATCVTHRLAGL
LSRSGGVVKNNFVPT
NVGSKAF
Nameα-CGRP (α-calcitonin gene-related peptide)
Length37
N-Terminal ModificationFree
C-Terminal ModificationFree
Linear/ CyclicLinear
ChiralityL
Chemical ModificationNone
Origin of PeptideCGRP is encoded in the same gene as calcitonin and expressed in neural tissues by processing through a different mRNA pathway
Nature of Peptide/CargoSpecific binding sites for CGRP have been demonstrated both in the brain and in peripheral target tissues, where CGRP has been shown to act through a CAMP-dependent mechanism
MechanismCGRP is encoded in the same gene as calcitonin and expressed in neural tissues by processing through a different mRNA pathway
Cargo Sequence/StructureNone
Name of cargo
Not applicable
AssayIntraocular pressure (IOP) calculated electromanometrically, blood-aqueous barrier, pupil size, blood pressure and cyclic AMP (CAMP) content in the aqueous humour of rabbits via 125I-radioimmunoassay
EnhancerNot mentioned
Properties of enhancerNot mentioned
ConcentrationCGRP=0.5µg, Contralateral eye=sensory nerve stimulation of the eye was achieved by topical application of 50 µl of 1% neutral formaldehyde (in 61 M phosphate buffer, pH 7.4) to the right eye, Control animals received intracameral saline correspondingly.
Incubation time30 minutes
Tissue permeability (value with units)IOP:Control=1.8mmHg, Neutral formaldehyde=From the baseline of 16.9±1.5 it rose by 27.3±3.8mmHg in 15.1±1.8 minutes, CGRP(0.5µg)=From the baseline of 18.5±1.1 it rose by 11.8±2.3mmHg in 15.0±2 minutes; Mean arterial blood pressure:Control=94±12/101±10(before), Formaldehyde=92±5/95±3(before), CGRP=94±5/98±4(before); cAMP after 30 minutes:Control=16.3±3.6pmol/ml, Formaldehyde right eye=88.5±35pmol/ml, Formaldehyde left e
Tissue SampleAlbino rabbits of both sexes weighing 2.2-3.1 kg were used. Right eye=Contralateral eye and left eye=Experimental.
Ex vivo/In vivo/In vitroin vivo
SECONDARY INFORMATION
STRUCTURE
Predicted Structure
SMILESN[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N
[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H]
(C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N
[C@H](C(=O)NCC(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H]
(C(=O)N[C@H](C(=O)N[C@H](C(=O)NCC(=O)NCC
(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H]
(C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C
(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)NCC
(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N
[C@H](C=O)Cc1ccccc1)C)CCCCN)CO)C(C)C)CC(=O)N)[C@H](O)
C)CCC)C(C)C)Cc1ccccc1)CC(=O)N)CC(=O)N)CCCCN)
C(C)C)C(C)C)CO)CCCNC(=N)N)CO)CC(C)C)CC(C)C)C)
CC(C)C)CCCNC(=N)N)Cc1[nH]cnc1)[C@H]
(O)C)C(C)C)CS)[C@H](O)C)C)[C@H](O)C)CC(=O)O)CS)C