Welcome to Entry Card of Peptide


Details of TopicalPdb ID 1145

PRIMARY INFORMATION
ID1145
PMID16113599
Year2007
SequenceSIINFEKL
NameOVA257–264
Length8
N-Terminal ModificationFree
C-Terminal ModificationFree
Linear/ CyclicLinear
ChiralityL
Chemical ModificationNone
Origin of PeptideSynthetic
Nature of Peptide/CargoIt is a class I (Kb)-restricted peptide epitope of ovalbumin (OVA), presented by the class I major histocompatibility complex (MHC) molecule, H-2Kb.
MechanismIt has been demonstrated that OVA 257-264 peptides can be used to detect a strong CD8+ cytolytic T cell response.
Cargo Sequence/Structure3-(2-Methylpropyl)-3,5,8-triazatricyclo[7.4.0.02,6]trideca-1(9),2(6),4,7,10,12-hexaen-7-amine
Name of cargo
Imiquimod
AssayELISPOT assay
Enhancer37°C for 24 hours during the assay, immunized once
Properties of enhancerImiquimod is a toll-like receptor agonist that functions as a potent adjuvant
Concentration300 μmol peptide(10 mg/mL stock)+35µl imiquimod(1.75mg)
Incubation time7 days
Tissue permeability (value with units)~ 210 spots/1*106 total cells(4 folds more than the permeability of the peptide with vehicle alone)
Tissue SampleEpidermal layer of naive C57BL mice
Ex vivo/In vivo/In vitroex vivo
SECONDARY INFORMATION
STRUCTURE
Predicted Structure
SMILESN[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N
[C@@H](CC(=O)N)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCC(=O)O)C(=O)
N[C@@H](CCCC[NH3])C(=O)N[C@@H](CC(C)C)C=O