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Details of TopicalPdb ID 1123

PRIMARY INFORMATION
ID1123
PMID18670091
Year2008
SequenceMNFVMISIELSETMAL
IIVISGLLLCASSITT
TSSNDFYAVLHGNLSQ
SDRNELPKICGREFLT
DTLQSRSAGGVLVREN
EYPWVLLLTDPEWTRV
CTAVLISRRHVLTAAH
CVTNFPKDRKLEKDCH
YTTIQSTYLYVYPRTR
VNDALNIKRYTSSFSV
ARVMVHPSFSCSNATG
DIALLELTLNIFTEAS
PICMPHFNESIPKNAA
AAGFGKNPISNNTRPM
QVVNLTYQGTTGDRI
NameTrypsin
Length293
N-Terminal ModificationFree
C-Terminal ModificationFree
Linear/ CyclicLinear
ChiralityL
Chemical ModificationNone
Origin of PeptideBovine
Nature of Peptide/CargoEndogenous proteases trypsin is capable of digesting intercellular desmosomal proteins and hence used for in vitro epidermal separation and keratinocyte isolation
MechanismThe unique ability of proteases to cause selective epidermal separation has been in part explained by the proteolytic degradation of desmosomal proteins in the SC, which leads to cell dissociation
Cargo Sequence/StructureMALWTRLAPLLALLALWAPAPARAFVNQHLCGSHLVEALYLVCGERGFFYTPKARREVEGQVGALELAGGPGAGGLEGPPQKRGIVEQCCASVCSLYQLENYCN
Name of cargo
Bovine insulin
AssayFranz diffusion cell
Enhancer0.1 M PBS, pH 7.4
Properties of enhancerNot applicable
Concentration200 µl of 0.25% trypsin (w/v)
Incubation time30 minutes
Tissue permeability (value with units)Permeability coefficient at 0.25% trypsin pretreatment is 5.29 X 107cm/s,permeation flux increased 5.2-fold.A higher concentration of trypsin shortened the lag time of insulin permeation
Tissue SampleEpidermal skin of male wistar rat
Ex vivo/In vivo/In vitroin vitro
SECONDARY INFORMATION
STRUCTURE
Predicted Structure
SMILESN.A.